Key features and details
- Rabbit polyclonal to RhoA (phospho S188)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
产品名称Anti-RhoA (phospho S188)抗体
参阅全部 RhoA 一抗
描述兔多克隆抗体to RhoA (phospho S188)
特异性Phospho-Ser188-RhoA specific. RhoA immunogen sequence not found in RhoB or RhoC proteins.
经测试应用适用于: WBmore details
Synthetic peptide corresponding to Human RhoA (phospho S188) conjugated to keyhole limpet haemocyanin.
Database link: P06749
常规说明Do not aliquot.
Post-translational regulation of Rho activity has been shown specifically for RhoA. This Rho protein is phosphorylated in vitro on serine 188 by cAMP- and cGMP-dependent kinases (PKA and PKG). Ser188 phosphorylation enhances RhoGDI binding and inhibits RhoA-mediated stress fiber formation. Thus, Ser188 is an important site for negative regulation of RhoA activity.
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存放说明Shipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C.
存储溶液Preservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol, 0.1% BSA
Concentration information loading...
纯度Immunogen affinity purified
Primary antibody说明Post-translational regulation of Rho activity has been shown specifically for RhoA. This Rho protein is phosphorylated in vitro on serine 188 by cAMP- and cGMP-dependent kinases (PKA and PKG). Ser188 phosphorylation enhances RhoGDI binding and inhibits RhoA-mediated stress fiber formation. Thus, Ser188 is an important site for negative regulation of RhoA activity.
功能Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. Serves as a target for the yopT cysteine peptidase from Yersinia pestis, vector of the plague, and Yersinia pseudotuberculosis, which causes gastrointestinal disorders. May be an activator of PLCE1. Activated by ARHGEF2, which promotes the exchange of GDP for GTP.
序列相似性Belongs to the small GTPase superfamily. Rho family.
结构域The basic-rich region is essential for yopT recognition and cleavage.
翻译后修饰Substrate for botulinum ADP-ribosyltransferase.
Cleaved by yopT protease when the cell is infected by some Yersinia pathogens. This removes the lipid attachment, and leads to its displacement from plasma membrane and to subsequent cytoskeleton cleavage.
AMPylation at Tyr-34 and Thr-37 are mediated by bacterial enzymes in case of infection by H.somnus and V.parahaemolyticus, respectively. AMPylation occurs in the effector region and leads to inactivation of the GTPase activity by preventing the interaction with downstream effectors, thereby inhibiting actin assembly in infected cells. It is unclear whether some human enzyme mediates AMPylation; FICD has such ability in vitro but additional experiments remain to be done to confirm results in vivo.
Ubiquitinated by the BCR(BACURD1) and BCR(BACURD2) E3 ubiquitin ligase complexes, leading to its degradation by the proteasome, thereby regulating the actin cytoskeleton and cell migration.
细胞定位Cell membrane. Cytoplasm > cytoskeleton.
- Information by UniProt
- Aplysia ras related homolog 12 antibody
- ARH12 antibody
- ARHA antibody
Lanes 1-2 : anti-Rho
Lanes 3-6 : Anti-RhoA (phospho S188) antibody (ab41435) at 1/1000 dilution
Lane 1 : recombinant human RhoA GST fusion protein, unphosphorylated
Lane 2 : recombinant human RhoA GST fusion protein, phosphorylated with PKA
Lane 3 : recombinant human RhoA GST fusion protein, unphosphorylated
Lane 4 : recombinant human RhoA GST fusion protein, phosphorylated with PKA
Lane 5 : recombinant human RhoA GST fusion protein, phosphorylated with PKA
with RhoA (phospho S188) peptide
Lane 6 : recombinant human RhoA GST fusion protein, phosphorylated with PKA with non-specific phosphoserine peptide
Predicted band size: 22 kDa
Observed band size: 49 kDa why is the actual band size different from the predicted?
Membrane was incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween20 for 1 hour at room temperature.
A fusion protein was used in this western blot; hence the difference in molecular weight compared to predicted molecular weight.
All lanes : Anti-RhoA (phospho S188) antibody (ab41435) at 1/1000 dilution
Lane 1 : MCF7
Lane 2 : human liver nuclear tissue lysate
Lysates/proteins at 20 µg per lane.
All lanes : Polyclonal goat anti-rabbit HRP conjugate at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 22 kDa
Observed band size: 22 kDa
Exposure time: 25 seconds
Blocking: 5% milk for 1 hour at 23°C
ab41435 被引用在 28 文献中.
- Liu X et al. Pre-miRNA Hsa-Let-7a-2: a Novel Intracellular Partner of Angiotensin II Type 2 Receptor Negatively Regulating its Signals. Int J Biol Sci 18:3237-3250 (2022). PubMed: 35637969
- Yan L et al. Mex-3 RNA binding MEX3A promotes the proliferation and migration of breast cancer cells via regulating RhoA/ROCK1/LIMK1 signaling pathway. Bioengineered 12:5850-5858 (2021). PubMed: 34486491
- Senoo H et al. KARATE: PKA-induced KRAS4B-RHOA-mTORC2 supercomplex phosphorylates AKT in insulin signaling and glucose homeostasis. Mol Cell 81:4622-4634.e8 (2021). PubMed: 34551282
- Bonnet C et al. Wnt6 plays a complex role in maintaining human limbal stem/progenitor cells. Sci Rep 11:20948 (2021). PubMed: 34686698
- Ke W et al. The distinct roles of myosin IIA and IIB under compression stress in nucleus pulposus cells. Cell Prolif 54:e12987 (2021). PubMed: 33415745