Anti-RhoA (phospho S188)抗体(ab41435)
Key features and details
- Rabbit polyclonal to RhoA (phospho S188)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-RhoA (phospho S188)抗体
参阅全部 RhoA 一抗 -
描述
兔多克隆抗体to RhoA (phospho S188) -
宿主
Rabbit -
特异性
Phospho-Ser188-RhoA specific. RhoA immunogen sequence not found in RhoB or RhoC proteins. -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human
预测可用于: Rat
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免疫原
Synthetic peptide corresponding to Human RhoA (phospho S188) conjugated to keyhole limpet haemocyanin.
Database link: P06749 -
常规说明
Do not aliquot.Post-translational regulation of Rho activity has been shown specifically for RhoA. This Rho protein is phosphorylated in vitro on serine 188 by cAMP- and cGMP-dependent kinases (PKA and PKG). Ser188 phosphorylation enhances RhoGDI binding and inhibits RhoA-mediated stress fiber formation. Thus, Ser188 is an important site for negative regulation of RhoA activity.
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性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C. -
存储溶液
Preservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol, 0.1% BSA -
Concentration information loading... -
纯度
Immunogen affinity purified -
Primary antibody说明
Post-translational regulation of Rho activity has been shown specifically for RhoA. This Rho protein is phosphorylated in vitro on serine 188 by cAMP- and cGMP-dependent kinases (PKA and PKG). Ser188 phosphorylation enhances RhoGDI binding and inhibits RhoA-mediated stress fiber formation. Thus, Ser188 is an important site for negative regulation of RhoA activity. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab41435于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (9) |
1/1000. Predicted molecular weight: 22 kDa.
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| 说明 |
|---|
|
WB
1/1000. Predicted molecular weight: 22 kDa. |
靶标
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功能
Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. Serves as a target for the yopT cysteine peptidase from Yersinia pestis, vector of the plague, and Yersinia pseudotuberculosis, which causes gastrointestinal disorders. May be an activator of PLCE1. Activated by ARHGEF2, which promotes the exchange of GDP for GTP. -
序列相似性
Belongs to the small GTPase superfamily. Rho family. -
结构域
The basic-rich region is essential for yopT recognition and cleavage. -
翻译后修饰
Substrate for botulinum ADP-ribosyltransferase.
Cleaved by yopT protease when the cell is infected by some Yersinia pathogens. This removes the lipid attachment, and leads to its displacement from plasma membrane and to subsequent cytoskeleton cleavage.
AMPylation at Tyr-34 and Thr-37 are mediated by bacterial enzymes in case of infection by H.somnus and V.parahaemolyticus, respectively. AMPylation occurs in the effector region and leads to inactivation of the GTPase activity by preventing the interaction with downstream effectors, thereby inhibiting actin assembly in infected cells. It is unclear whether some human enzyme mediates AMPylation; FICD has such ability in vitro but additional experiments remain to be done to confirm results in vivo.
Ubiquitinated by the BCR(BACURD1) and BCR(BACURD2) E3 ubiquitin ligase complexes, leading to its degradation by the proteasome, thereby regulating the actin cytoskeleton and cell migration. -
细胞定位
Cell membrane. Cytoplasm > cytoskeleton. - Information by UniProt
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数据库链接
- Entrez Gene: 387 Human
- Entrez Gene: 117273 Rat
- Omim: 165390 Human
- SwissProt: P61586 Human
- SwissProt: P61589 Rat
- Unigene: 247077 Human
- Unigene: 107401 Rat
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别名
- Aplysia ras related homolog 12 antibody
- ARH12 antibody
- ARHA antibody
see all
图片
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Western blot - Anti-RhoA (phospho S188) antibody (ab41435)Lanes 1-2 : anti-Rho
Lanes 3-6 : Anti-RhoA (phospho S188) antibody (ab41435) at 1/1000 dilution
Lane 1 : recombinant human RhoA GST fusion protein, unphosphorylated
Lane 2 : recombinant human RhoA GST fusion protein, phosphorylated with PKA
Lane 3 : recombinant human RhoA GST fusion protein, unphosphorylated
Lane 4 : recombinant human RhoA GST fusion protein, phosphorylated with PKA
Lane 5 : recombinant human RhoA GST fusion protein, phosphorylated with PKA
with RhoA (phospho S188) peptide
Lane 6 : recombinant human RhoA GST fusion protein, phosphorylated with PKA with non-specific phosphoserine peptide
Predicted band size: 22 kDa
Observed band size: 49 kDa why is the actual band size different from the predicted?
Membrane was incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween20 for 1 hour at room temperature.A fusion protein was used in this western blot; hence the difference in molecular weight compared to predicted molecular weight.
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Western blot - Anti-RhoA (phospho S188) antibody (ab41435)This image is courtesy of an anonymous abreview.All lanes : Anti-RhoA (phospho S188) antibody (ab41435) at 1/1000 dilution
Lane 1 : MCF7
Lane 2 : human liver nuclear tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Polyclonal goat anti-rabbit HRP conjugate at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 22 kDa
Observed band size: 22 kDa
Exposure time: 25 secondsBlocking: 5% milk for 1 hour at 23°C
数据表及文件
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SDS download
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Datasheet download
文献 (28)
ab41435 被引用在 28 文献中.
- Liu X et al. Pre-miRNA Hsa-Let-7a-2: a Novel Intracellular Partner of Angiotensin II Type 2 Receptor Negatively Regulating its Signals. Int J Biol Sci 18:3237-3250 (2022). PubMed: 35637969
- Yan L et al. Mex-3 RNA binding MEX3A promotes the proliferation and migration of breast cancer cells via regulating RhoA/ROCK1/LIMK1 signaling pathway. Bioengineered 12:5850-5858 (2021). PubMed: 34486491
- Senoo H et al. KARATE: PKA-induced KRAS4B-RHOA-mTORC2 supercomplex phosphorylates AKT in insulin signaling and glucose homeostasis. Mol Cell 81:4622-4634.e8 (2021). PubMed: 34551282
- Bonnet C et al. Wnt6 plays a complex role in maintaining human limbal stem/progenitor cells. Sci Rep 11:20948 (2021). PubMed: 34686698
- Ke W et al. The distinct roles of myosin IIA and IIB under compression stress in nucleus pulposus cells. Cell Prolif 54:e12987 (2021). PubMed: 33415745