重组Anti-Rho抗体[Y486] (ab32046)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [Y486] to Rho
- Suitable for: WB, IHC-P
- Reacts with: Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-Rho抗体[Y486]
参阅全部 Rho 一抗 -
描述
兔单克隆抗体[Y486] to Rho -
宿主
Rabbit -
特异性
The antibody is predicted to detect RhoA, RhoB and RhoC based on sequence analysis. -
经测试应用
适用于: WB, IHC-Pmore details
不适用于: Flow Cyt or IP -
种属反应性
与反应: Rat, Human
预测可用于: Mouse -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- PC12 cell lysate and hepatocellular carcinoma.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
Y486 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab32046于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/1000 - 1/10000. Detects a band of approximately 22 kDa (predicted molecular weight: 21 kDa).
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IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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说明 |
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WB
1/1000 - 1/10000. Detects a band of approximately 22 kDa (predicted molecular weight: 21 kDa). |
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. Involved in a microtubule-dependent signal that is required for the myosin contractile ring formation during cell cycle cytokinesis. Plays an essential role in cleavage furrow formation. Required for the apical junction formation of keratinocyte cell-cell adhesion. Serves as a target for the yopT cysteine peptidase from Yersinia pestis, vector of the plague, and Yersinia pseudotuberculosis, which causes gastrointestinal disorders. Stimulates PKN2 kinase activity. May be an activator of PLCE1. Activated by ARHGEF2, which promotes the exchange of GDP for GTP. Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly. The MEMO1-RHOA-DIAPH1 signaling pathway plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. It controls the localization of APC and CLASP2 to the cell membrane, via the regulation of GSK3B activity. In turn, membrane-bound APC allows the localization of the MACF1 to the cell membrane, which is required for microtubule capture and stabilization. -
序列相似性
Belongs to the small GTPase superfamily. Rho family. -
结构域
The basic-rich region is essential for yopT recognition and cleavage. -
翻译后修饰
Substrate for botulinum ADP-ribosyltransferase.
Cleaved by yopT protease when the cell is infected by some Yersinia pathogens. This removes the lipid attachment, and leads to its displacement from plasma membrane and to subsequent cytoskeleton cleavage.
AMPylation at Tyr-34 and Thr-37 are mediated by bacterial enzymes in case of infection by H.somnus and V.parahaemolyticus, respectively. AMPylation occurs in the effector region and leads to inactivation of the GTPase activity by preventing the interaction with downstream effectors, thereby inhibiting actin assembly in infected cells. It is unclear whether some human enzyme mediates AMPylation; FICD has such ability in vitro but additional experiments remain to be done to confirm results in vivo.
Phosphorylation by PRKG1 at Ser-188 inactivates RHOA signaling.
Ubiquitinated by the BCR(BACURD1) and BCR(BACURD2) E3 ubiquitin ligase complexes, leading to its degradation by the proteasome, thereby regulating the actin cytoskeleton and cell migration. -
细胞定位
Cell membrane. Cytoplasm > cytoskeleton. Cleavage furrow. Cytoplasm > cell cortex. Midbody. Localized to cell-cell contacts in calcium-treated keratinocytes (By similarity). Translocates to the equatorial region before furrow formation in a ECT2-dependent manner. Localizes to the equatorial cell cortex (at the site of the presumptive furrow) in early anaphase in a activated form and in a myosin- and actin-independent manner. - Information by UniProt
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数据库链接
- Entrez Gene: 387 Human
- Entrez Gene: 388 Human
- Entrez Gene: 389 Human
- Entrez Gene: 11848 Mouse
- Entrez Gene: 117273 Rat
- SwissProt: P08134 Human
- SwissProt: P61586 Human
- SwissProt: P62745 Human
see all -
别名
- ARH12 antibody
- ARHA antibody
- H12 antibody
see all
图片
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Anti-Rho antibody [Y486] (ab32046) at 1/2000 dilution + PC12 cell lysate
Predicted band size: 21 kDa
Observed band size: 22 kDa why is the actual band size different from the predicted? -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rho antibody [Y486] (ab32046)
Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma using ab32046 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (10)
ab32046 被引用在 10 文献中.
- Wang X et al. Yes-associated protein reacts differently in vascular smooth muscle cells under different intensities of mechanical stretch. Aging (Albany NY) 14:286-296 (2022). PubMed: 34983026
- Huang L et al. New In Vitro Cellular Model for Molecular Studies of Retinitis Pigmentosa. Int J Mol Sci 22:N/A (2021). PubMed: 34208617
- Bai Y et al. The diagnostic and prognostic role of RhoA in hepatocellular carcinoma. Aging (Albany NY) 11:5158-5172 (2019). PubMed: 31339860
- Vighi E et al. New cGMP analogues restrain proliferation and migration of melanoma cells. Oncotarget 9:5301-5320 (2018). PubMed: 29435180
- Soon PS et al. Profiling differential microRNA expression between in situ, infiltrative and lympho-vascular space invasive breast cancer: a pilot study. Clin Exp Metastasis 35:3-13 (2018). PubMed: 29214365
- Garcia-Vidal E et al. Evaluation of the Innate Immune Modulator Acitretin as a Strategy To Clear the HIV Reservoir. Antimicrob Agents Chemother 61:N/A (2017). PubMed: 28874382
- Greenaway JB et al. Ovarian tumour growth is characterized by mevalonate pathway gene signature in an orthotopic, syngeneic model of epithelial ovarian cancer. Oncotarget 7:47343-47365 (2016). WB, IF . PubMed: 27329838
- Girouard MP et al. RhoA Proteolysis Regulates the Actin Cytoskeleton in Response to Oxidative Stress. PLoS One 11:e0168641 (2016). PubMed: 27992599
- Ruggeri RM et al. The tyrosine kinase receptor c-met, its cognate ligand HGF and the tyrosine kinase receptor trasducers STAT3, PI3K and RHO in thyroid nodules associated with Hashimoto's thyroiditis: an immunohistochemical characterization. Eur J Histochem 54:e24 (2010). PubMed: 20558345
- Laumanns IP et al. The noncanonical WNT pathway is operative in idiopathic pulmonary arterial hypertension. Am J Respir Cell Mol Biol 40:683-91 (2009). PubMed: 19029018