Anti-RHEB抗体(ab25873)
Key features and details
- Rabbit polyclonal to RHEB
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Mouse, Rat, Human, Drosophila melanogaster
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-RHEB抗体
参阅全部 RHEB 一抗 -
描述
兔多克隆抗体to RHEB -
宿主
Rabbit -
特异性
ab25873 recognises Rheb (Ras homolog enriched in brain). -
经测试应用
适用于: IHC-P, ICC/IF, WBmore details -
种属反应性
与反应: Mouse, Rat, Human, Drosophila melanogaster -
免疫原
A 15 amino acid peptide from the middle region of Human Rheb.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
存储溶液
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab25873于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
Use a concentration of 2 µg/ml.
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| ICC/IF |
Use at an assay dependent concentration.
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| WB | (1) |
Use a concentration of 1 µg/ml. Predicted molecular weight: 20 kDa.
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| 说明 |
|---|
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IHC-P
Use a concentration of 2 µg/ml. |
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ICC/IF
Use at an assay dependent concentration. |
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WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 20 kDa. |
靶标
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功能
Stimulates the phosphorylation of S6K1 and EIF4EBP1 through activation of mTORC1 signaling. Activates the protein kinase activity of mTORC1. Has low intrinsic GTPase activity. -
组织特异性
Ubiquitous. Highest levels observed in skeletal and cardiac muscle. -
序列相似性
Belongs to the small GTPase superfamily. Rheb family. -
翻译后修饰
Farnesylation is important for efficiently activating mTORC1-mediated signaling. -
细胞定位
Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 6009 Human
- Entrez Gene: 19744 Mouse
- Entrez Gene: 26954 Rat
- Omim: 601293 Human
- SwissProt: Q15382 Human
- SwissProt: Q921J2 Mouse
- SwissProt: Q62639 Rat
- Unigene: 283521 Human
see all -
别名
- Ras homolog enriched in brain 2, formerly antibody
- GTP binding protein Rheb antibody
- GTP-binding protein Rheb antibody
see all
图片
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Western blot - Anti-RHEB antibody (ab25873)Western blot analysis of Rheb in mouse brain cell lysate with ab25873 at (A) 1, (B) 2, and (C) 4 ug/ml.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RHEB antibody (ab25873)ab25873 at 2µg/ml staining RHEB in mouse brain tissue by IHC
数据表及文件
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SDS download
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Datasheet download
文献 (17)
ab25873 被引用在 17 文献中.
- Sun X et al. Mechanistic target of rapamycin complex 1 orchestrates the interplay between hepatocytes and Kupffer cells to determine the outcome of immune-mediated hepatitis. Cell Death Dis 13:1031 (2022). PubMed: 36494334
- Lu Q et al. Complement factor B in high glucose-induced podocyte injury and diabetic kidney disease. JCI Insight 6:N/A (2021). PubMed: 34622800
- Li Z et al. miR-155-5p upregulation ameliorates myocardial insulin resistance via mTOR signaling in chronic alcohol drinking rats. PeerJ 9:e10920 (2021). PubMed: 33868799
- Tang L et al. Electroacupuncture Upregulated Ghrelin in Rats with Functional Dyspepsia via AMPK/TSC2/Rheb-Mediated mTOR Inhibition. Dig Dis Sci 65:1689-1699 (2020). PubMed: 31863340
- Li Z et al. miR-155-5p alleviates ethanol-induced myocardial insulin resistance in H9C2 cells via regulating the mTOR signalling pathway. Mol Biol Rep 47:9469-9477 (2020). PubMed: 33159675