AB247728

重组Anti-RGS9抗体[EPR2873] - BSA and Azide free

Anti-RGS9 antibody [EPR2873] - BSA and Azide free

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal RGS9 antibody. Carrier free. Suitable for ICC/IF, WB, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples. Cited in 1 publication.

查看别名

Regulator of G-protein signaling 9, RGS9

6 Images

Flow Cytometry (Intracellular) - Anti-RGS9 antibody [EPR2873] - BSA and Azide free (AB247728)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-RGS9 antibody [EPR2873] - BSA and Azide free (AB247728)

This data was developed using ab108975, the same antibody clone in a different buffer formulation.

Intracellular Flow Cytometry analysis of Y79 cells labelling RGS9 with purified ab108975 at a dilution of 1/60 (red). Cells were fixed with 4% paraformaldehyde. An Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.

Immunocytochemistry/ Immunofluorescence - Anti-RGS9 antibody [EPR2873] - BSA and Azide free (AB247728)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-RGS9 antibody [EPR2873] - BSA and Azide free (AB247728)

This data was developed using ab108975, the same antibody clone in a different buffer formulation.

Immunocytochemistry/Immunofluorescence analysis of Neuro-2a cells labelling RGS9 with purified ab108975 at a dilution of 1/100. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor^® 594-conjugated goat anti-mouse IgG (1/1000) were also used.

Control 1 : primary antibody (1/100) and secondary antibody, ab150120, an Alexa Fluor^® 594-conjugated goat anti-mouse IgG (1/1000).

Control 2 : ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/1000).

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-RGS9 antibody [EPR2873] - BSA and Azide free (AB247728)

This data was developed using ab108975, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of permeabilized PC12 cells labelling RGS9 with unpurified ab108975 (red) at a dilution of 1/30 or a rabbit IgG (negative) (green).

Lab

Western blot - Anti-RGS9 antibody [EPR2873] - BSA and Azide free (AB247728)

This data was developed using ab108975, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-RGS9 antibody [EPR2873] (<a href='/products/primary-antibodies/rgs9-antibody-epr2873-ab108975'>ab108975</a>) at 1/1000 dilution

Lane 1:

Y79 whole cell lysate at 20 µg

Lane 2:

Mouse spleen tissue lysate at 20 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 77 kDa

Observed band size: 55 kDa

false

Unknown

Western blot - Anti-RGS9 antibody [EPR2873] - BSA and Azide free (AB247728)

This data was developed using ab108975, the same antibody clone in a different buffer formulation.

Note : Predicted molecular weights for UniProt ID O75916 isoforms 1 to 4 are ~77, 55, 57 and 50 kDa respectively. Therefore, the observed WB band may correspond to isoform 2, 3 or 4.

All lanes:

Western blot - Anti-RGS9 antibody [EPR2873] (<a href='/products/primary-antibodies/rgs9-antibody-epr2873-ab108975'>ab108975</a>) at 1/1000 dilution

Lane 1:

PC12 lysates at 10 µg

Lane 2:

Human spleen lysates at 10 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

Predicted band size: 77 kDa

Observed band size: 55 kDa

false

Lab

Western blot - Anti-RGS9 antibody [EPR2873] - BSA and Azide free (AB247728)

This data was developed using ab108975, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-RGS9 antibody [EPR2873] (<a href='/products/primary-antibodies/rgs9-antibody-epr2873-ab108975'>ab108975</a>) at 1/1000 dilution

All lanes:

PC-12 whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 77 kDa

Observed band size: 55 kDa

false

不同偶联物与剂型 (1)

Unconjugated

Anti-RGS9 antibody [EPR2873]

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR2873

亚型

IgG

不含载体蛋白

Yes

反应种属

Mouse, Rat, Human

应用

WB, ICC/IF, Flow Cyt (Intra)

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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产品详情

ab247728 is the carrier-free version of ab108975.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Constituents: PBS

运输条件

Blue Ice

储存信息

Do Not Freeze

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Regulator of G protein Signaling 9 or RGS9 plays an important role in the regulation of G protein-coupled receptor (GPCR) signaling pathways. RGS9 acts as a GTPase-activating protein (GAP) accelerating the deactivation of Gα subunits which helps terminate the signal transduction. This protein with a molecular mass of roughly 73 kDa is highly expressed in the brain especially in the striatum and in the retina. These tissues suggest its specialized involvement in visual and neurological functions.

Biological function summary

RGS9 functions as an important regulator within multiple signaling complexes. It associates with RGS9-binding protein (R9AP) and Gβ5 subunits to form larger complexes that enhance its activity. RGS9 regulates phototransduction in rod and cone photoreceptors by controlling the inactivation of transducin a heterotrimeric G protein involved in visual processing. This regulation ensures proper recovery of photoreceptors after light-induced activation maintaining visual acuity and stability.

Pathways

RGS9 is integral to visual signal transduction and dopaminergic signaling pathways. In the visual system it modulates the phototransduction cascade by interacting with transducin within the rod outer segment. Within dopamine pathways it affects signaling by modulating receptors like D2 dopamine receptors involving proteins such as Gαolf which mediates cAMP production. These interactions regulate neuronal responses to various stimuli impacting vision and limbic system functions.

RGS9 abnormalities relate to conditions like bradyopsia and drug addiction. Bradyopsia characterized by slow vision recovery after light exposure is linked to RGS9 mutations which disrupt normal photoreceptor recovery. In addiction altered RGS9 expression impacts dopamine signaling affecting addiction pathways with involvement of D2 dopamine receptors. Together these insights highlight RGS9's critical role in maintaining normal visual and neurological health.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP-bound form. Binds to GNAT1. Involved in phototransduction; key element in the recovery phase of visual transduction (By similarity).

See full target information Regulator of G-protein signaling 9

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Oncology letters 26:409 PubMed37600337

2023

Renal epithelioid angiomyolipoma: A case report.

Applications

Unspecified application

Species

Unspecified reactive species

Hongyun Li,Xuebei Zhang,Qingli Zhao,Jin Wang,Xiaoqing Yang,Shengliang Huang

View all publications

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Abcam Product Promise

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详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

重组Anti-RGS9抗体[EPR2873] - BSA and Azide free