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Anti-RGS16抗体[OTI4E5] (ab119424)

Submit a review Submit a question References (3)
Western blot - Anti-RGS16 antibody [OTI4E5] (ab119424)
  • Western blot - Anti-RGS16 antibody [OTI4E5] (ab119424)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RGS16 antibody [OTI4E5] (ab119424)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RGS16 antibody [OTI4E5] (ab119424)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RGS16 antibody [OTI4E5] (ab119424)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RGS16 antibody [OTI4E5] (ab119424)
  • Immunocytochemistry/ Immunofluorescence - Anti-RGS16 antibody [OTI4E5] (ab119424)
  • Flow Cytometry - Anti-RGS16 antibody [OTI4E5] (ab119424)

Key features and details

  • Mouse monoclonal [OTI4E5] to RGS16
  • Suitable for: WB, IHC-P, Flow Cyt, ICC/IF
  • Reacts with: Mouse, Human, African green monkey
  • Isotype: IgG1

概述

  • 产品名称

    Anti-RGS16抗体[OTI4E5]

  • 描述

    小鼠单克隆抗体[OTI4E5] to RGS16

  • 宿主

    Mouse

  • 经测试应用

    适用于: WB, IHC-P, Flow Cyt, ICC/IFmore details

  • 种属反应性

    与反应: Mouse, Human, African green monkey

  • 免疫原

    Recombinant full length protein corresponding to Human RGS16 aa 1-250. Produced in HEK293T cells (NP_002919).
    Database link: O15492

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • 阳性对照

    • WB: HEK-293T cell lysate transfected with pCMV6-ENTRY RGS16 cDNA; SVT2 cell extract. IHC-P: Human breast, Kidney, Pancreas and Endometrium tissues. ICC/IF: COS7 cells transiently transfected with pCMV6-ENTRY RGS16. Flow: Jurkat cells.

  • 常规说明

    Clone OTI4E5 (formerly 4E5).

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • 存储溶液

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 50% Glycerol, 1% BSA
  • Concentration information loading...

  • 纯度

    Affinity purified

  • 纯化说明

    Purified from cell culture supernatant by affinity chromatography

  • 克隆

    单克隆

  • 克隆编号

    OTI4E5

  • 同种型

    IgG1

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab119424于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
1/500 - 1/2000. Predicted molecular weight: 23 kDa.
IHC-P
1/150.
Flow Cyt
1/100.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ICC/IF
1/100.
说明
WB
1/500 - 1/2000. Predicted molecular weight: 23 kDa.
IHC-P
1/150.
Flow Cyt
1/100.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ICC/IF
1/100.

靶标

  • 功能

    Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP-bound form. Binds to G(i)-alpha and G(o)-alpha, but not to G(s)-alpha. May play a role in regulating the kinetics of signaling in the phototransduction cascade.

  • 组织特异性

    Abundantly expressed in retina with lower levels of expression in most other tissues.

  • 序列相似性

    Contains 1 RGS domain.

  • 翻译后修饰

    Palmitoylated on Cys-2 and/or Cys-12.
    Phosphorylation on Tyr-168 upon EGFR stimulation. Enhanced GTPase accelerating (GAP) activity on G(i)-alpha.
  • Information by UniProt

  • 数据库链接

    • 别名

      • A28 RGS14 antibody
      • A28 RGS14P antibody
      • A28-RGS14P antibody
      • HGNC:9997 antibody
      • hRGS-r antibody
      • OTTHUMP00000033147 antibody
      • Regulator of G protein signaling 16 antibody
      • Regulator of G protein signalling 16 antibody
      • Regulator of G-protein signaling 16 antibody
      • Retinal-specific RGS antibody
      • Retinally abundant regulator of G protein signaling antibody
      • Retinally abundant regulator of G-protein signaling antibody
      • RGS 16 antibody
      • RGS R antibody
      • RGS-R antibody
      • Rgs14 antibody
      • RGS16 antibody
      • RGS16_HUMAN antibody
      • RGSR antibody
      see all

    图片

    • Western blot - Anti-RGS16 antibody [OTI4E5] (ab119424)
      Western blot - Anti-RGS16 antibody [OTI4E5] (ab119424)
      All lanes : Anti-RGS16 antibody [OTI4E5] (ab119424) at 1/500 dilution

      Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate transfected with pCMV6-ENTRY control cDNA
      Lane 2 : HEK-293T cell lysate transfected with pCMV6-ENTRY RGS16 cDNA

      Lysates/proteins at 5 µg per lane.

      Predicted band size: 23 kDa

    • Western blot - Anti-RGS16 antibody [OTI4E5] (ab119424)
      Western blot - Anti-RGS16 antibody [OTI4E5] (ab119424)
      All lanes : Anti-RGS16 antibody [OTI4E5] (ab119424) at 1/500 dilution

      Lane 1 : HepG2 (human liver hepatocellular carcinoma cell line) cell lysate
      Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
      Lane 3 : SVT2 cell extract
      Lane 4 : A549 (human lung carcinoma cell line) cell lysate
      Lane 5 : COS-7 (African green monkey kidney fibroblast-like cell line) cell lysate
      Lane 6 : Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate
      Lane 7 : MDCK cell extract
      Lane 8 : PC-12 (rat adrenal gland pheochromocytoma cell line) cell lysate
      Lane 9 : MCF7 (human breast adenocarcinoma cell line) cell lysate

      Lysates/proteins at 35 µg per lane.

      Predicted band size: 23 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RGS16 antibody [OTI4E5] (ab119424)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RGS16 antibody [OTI4E5] (ab119424)

      Paraffin-embedded human breast tissue stained for RGS16 using ab119424 at 1/150 dilution in immunohistochemical analysis.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RGS16 antibody [OTI4E5] (ab119424)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RGS16 antibody [OTI4E5] (ab119424)

      Paraffin-embedded human kidney tissue stained for RGS16 using ab119424 at 1/150 dilution in immunohistochemical analysis.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RGS16 antibody [OTI4E5] (ab119424)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RGS16 antibody [OTI4E5] (ab119424)

      Paraffin-embedded human pancreas tissue stained for RGS16 using ab119424 at 1/150 dilution in immunohistochemical analysis.

       

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RGS16 antibody [OTI4E5] (ab119424)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RGS16 antibody [OTI4E5] (ab119424)

      Paraffin-embedded human endometrium tissue stained for RGS16 using ab119424 at 1/150 dilution in immunohistochemical analysis.

    • Immunocytochemistry/ Immunofluorescence - Anti-RGS16 antibody [OTI4E5] (ab119424)
      Immunocytochemistry/ Immunofluorescence - Anti-RGS16 antibody [OTI4E5] (ab119424)

      COS-7 (African green monkey kidney fibroblast-like cell line) cells transiently transfected with pCMV6-ENTRY RGS16, stained for RGS16 (green) using ab119424 at 1/100 dilution in ICC/IF.

    • Flow Cytometry - Anti-RGS16 antibody [OTI4E5] (ab119424)
      Flow Cytometry - Anti-RGS16 antibody [OTI4E5] (ab119424)

      Flow cytometric analysis of jurkat (human T cell leukemia cell line from peripheral blood) cells staining RGS16 (red) using ab119424 at 1/100 dilution compared to a nonspecific negative control antibody (Blue).

    实验方案

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    文献 (3)

    发表研究结果有使用 ab119424？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab119424 被引用在 3 文献中.

    • Yu B  et al. Extracellular vesicles engineering by silicates-activated endothelial progenitor cells for myocardial infarction treatment in male mice. Nat Commun 14:2094 (2023). PubMed: 37055411
    • Zhu F  et al. Critical roles of RGS16 in the mucosal inflammation of ulcerative colitis. Eur J Gastroenterol Hepatol 34:993-999 (2022). PubMed: 35830366
    • Xu C  et al. ATE1 Inhibits Liver Cancer Progression through RGS5-Mediated Suppression of Wnt/ß-Catenin Signaling. Mol Cancer Res 19:1441-1453 (2021). PubMed: 34158395

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