Anti-RFRP抗体(ab122738)
Key features and details
- Rabbit polyclonal to RFRP
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-RFRP抗体
参阅全部 RFRP 一抗 -
描述
兔多克隆抗体to RFRP -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Recombinant fragment corresponding to Human RFRP aa 105-176.
Sequence:TANLPLRSGRNMEVSLVRRVPNLPQRFGRTTTAKSVCRMLSDLCQGSMHS PCANDLFYSMTCQHQEIQNPDQ
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阳性对照
- IHC-P:Human retina tissue; WB: RT4 and U-251 MG whole cells, human liver and tonsil tissue lysates.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab122738于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use a concentration of 0.04 - 0.4 µg/ml.
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| IHC-P |
1/1000 - 1/2500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
| 说明 |
|---|
|
WB
Use a concentration of 0.04 - 0.4 µg/ml. |
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IHC-P
1/1000 - 1/2500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
Neuropeptide RFRP-1 acts as a potent negative regulator of gonadotropin synthesis and secretion. Neuropeptides NPSF and NPVF efficiently inhibit forskolin-induced production of cAMP, but RFRP-2 shows no inhibitory activity. Neuropeptide RFRP-1 induces secretion of prolactin in rats. Neuropeptide NPVF blocks morphine-induced analgesia. -
组织特异性
Isoform 1 is specifically expressed in the retina. Neuropeptides RFRP-1 and NPVF are detected in the hypothalamus. -
序列相似性
Belongs to the FARP (FMRFamide related peptide) family. -
细胞定位
Secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 64111 Human
- SwissProt: Q9HCQ7 Human
- Unigene: 60473 Human
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别名
- C7orf9 antibody
- Neuropeptide NPSF antibody
- Neuropeptide NPVF antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RFRP antibody (ab122738)
Immunohistochemical analysis of human retina tissue labeling RFRP with ab122738 at a 1/1000 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Western blot - Anti-RFRP antibody (ab122738)All lanes : Anti-RFRP antibody (ab122738) at 0.4 µg/ml
Lane 1 : RT4 (Human urinary bladder cancer cell line) whole cell lysate
Lane 2 : U-251 MG (formally U-373 MG) (Human brain glioma cell line) whole cell lysate
Lane 3 : Human plasma tissue lysate
Lane 4 : Human liver tissue lysate
Lane 5 : Human tonsil tissue lysate
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab122738 被引用在 2 文献中.
- Lee DA et al. Evolutionarily conserved regulation of sleep by epidermal growth factor receptor signaling. Sci Adv 5:eaax4249 (2019). PubMed: 31763451
- Zhang L et al. Green light inhibits GnRH-I expression by stimulating the melatonin-GnIH pathway in the chick brain. J Neuroendocrinol 29:N/A (2017). PubMed: 28295740