Anti-RFP抗体(ab62341)
Key features and details
- Rabbit polyclonal to RFP
- Suitable for: WB
- Reacts with: Species independent
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-RFP抗体
参阅全部 RFP 一抗 -
描述
兔多克隆抗体to RFP -
宿主
Rabbit -
特异性
ab62341 recognizes RFP and has been shown to react with tdTomato. -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Species independent -
免疫原
Recombinant full length protein corresponding to RFP.
Database link: Q9U6Y8 -
常规说明
This product is manufactured by BioVision, an Abcam company and was previously called 3993 RFP (dsRed) Antibody. 3993-100 is the same size as the 100 µg size of ab62341.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
Preservative: 0.03% Proclin 300
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 0.5% BSA, 0.146% EDTA -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab62341于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (8) |
Use a concentration of 0.5 - 4 µg/ml. Predicted molecular weight: 27.6 kDa.
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| 说明 |
|---|
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WB
Use a concentration of 0.5 - 4 µg/ml. Predicted molecular weight: 27.6 kDa. |
靶标
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相关性
Fluorescent proteins have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions. -
别名
- GFP like chromoprotein antibody
- red fluorescent protein antibody
- RFP antibody
图片
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Western blot - Anti-RFP antibody (ab62341)All lanes : Anti-RFP antibody (ab62341) at 1 µg/ml
Lane 1 : Recombinant RFP 2ng
Lane 2 : Recombinant RFP 10ng
Lane 3 : Recombinant RFP 50ng
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/50000 dilution
Predicted band size: 27.6 kDa
Observed band size: 32 kDa why is the actual band size different from the predicted?Blocking Buffer - 3% Milk
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (320)
ab62341 被引用在 320 文献中.
- Liau ES et al. Single-cell transcriptomic analysis reveals diversity within mammalian spinal motor neurons. Nat Commun 14:46 (2023). PubMed: 36596814
- Azumane M et al. Semaphorin-RhoA signaling regulates HERS maintenance by acting against TGF-β-induced EMT. J Periodontal Res 58:184-194 (2023). PubMed: 36517910
- Faber S et al. PDE6D Mediates Trafficking of Prenylated Proteins NIM1K and UBL3 to Primary Cilia. Cells 12:N/A (2023). PubMed: 36672247
- Zhang Y et al. Axon targeting of Drosophila medulla projection neurons requires diffusible Netrin and is coordinated with neuroblast temporal patterning. Cell Rep 42:112144 (2023). PubMed: 36821439
- Zhang Y et al. Notch-dependent binary fate choice regulates the Netrin pathway to control axon guidance of Drosophila visual projection neurons. Cell Rep 42:112143 (2023). PubMed: 36821442