重组Anti-RFC2抗体[EPR12093(B)] (ab174271)
![Western blot - Anti-RFC2 antibody [EPR12093(B)] (ab174271)](https://www.abcam.cn/ps/products/174/ab174271/Images/ab174271-199146-anti-rfc2-antibody-epr12093b-western-blot.jpg "Western blot - Anti-RFC2 antibody [EPR12093(B)] (ab174271)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12093(B)] to RFC2
- Suitable for: Flow Cyt (Intra), WB, ICC/IF
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-RFC2抗体[EPR12093(B)]
参阅全部 RFC2 一抗 -
描述
兔单克隆抗体[EPR12093(B)] to RFC2 -
宿主
Rabbit -
经测试应用
适用于: Flow Cyt (Intra), WB, ICC/IFmore details
不适用于: IHC-P or IP -
种属反应性
与反应: Human
预测可用于: Mouse, Rat
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免疫原
Synthetic peptide within Human RFC2 aa 200-300 (Cysteine residue). The exact sequence is proprietary.
Database link: P35250 -
阳性对照
- HepG2, K562, MCF7, 293T, and HeLa whole cell lysate (ab150035); MCF7 and K562 cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
EPR12093(B) -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab174271于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
| WB |
1/1000 - 1/10000. Detects a band of approximately 40 kDa (predicted molecular weight: 39 kDa).
|
|
| ICC/IF |
1/100 - 1/250.
|
| 说明 |
|---|
|
Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB
1/1000 - 1/10000. Detects a band of approximately 40 kDa (predicted molecular weight: 39 kDa). |
|
ICC/IF
1/100 - 1/250. |
应用说明
Is unsuitable for IHC-P or IP.
靶标
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功能
The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins proliferating cell nuclear antigen (PCNA) and activator 1. This subunit binds ATP. -
疾病相关
Note=RFC2 is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region. -
序列相似性
Belongs to the activator 1 small subunits family. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 5982 Human
- Entrez Gene: 19718 Mouse
- Entrez Gene: 116468 Rat
- Omim: 600404 Human
- SwissProt: P35250 Human
- SwissProt: Q9WUK4 Mouse
- SwissProt: Q641W4 Rat
- Unigene: 647062 Human
see all -
别名
- A1 40 kDa subunit antibody
- A1 antibody
- Activator 1 40 kDa subunit antibody
see all
图片
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Western blot - Anti-RFC2 antibody [EPR12093(B)] (ab174271)All lanes : Anti-RFC2 antibody [EPR12093(B)] (ab174271) at 1/1000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : K562 cell lysate
Lane 3 : MCF7 cell lysate
Lane 4 : 293T (Human embryonic kidney epithelial cell) cell lysate
Lane 5 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 39 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-RFC2 antibody [EPR12093(B)] (ab174271)](https://www.abcam.cn/ps/products/174/ab174271/Images/ab174271-199145-anti-rfc2-antibody-epr12093b-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-RFC2 antibody [EPR12093(B)] (ab174271)Immunofluorescent analysis of MCF7 cells labeling RFC2 with ab174271 at 1/100 dilution.
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![Flow Cytometry (Intracellular) - Anti-RFC2 antibody [EPR12093(B)] (ab174271)](https://www.abcam.cn/ps/products/174/ab174271/Images/ab174271-199144-anti-rfc2-antibody-epr12093b-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-RFC2 antibody [EPR12093(B)] (ab174271)Intracellular flow cytometric analysis of permeabilized HeLa cells labeling RFC2 with ab174271at 1/10 dilution (red) or a rabbit IgG (negative) (green).
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![Anti-RFC2 antibody [EPR12093(B)] (ab174271)](https://www.abcam.cn/ps/products/174/ab174271/Images/ab174271-4-benefits-of-recombinant-antibodies.png)
Anti-RFC2 antibody [EPR12093(B)] (ab174271)
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab174271 被引用在 4 文献中.
- Liu L et al. An RFC4/Notch1 signaling feedback loop promotes NSCLC metastasis and stemness. Nat Commun 12:2693 (2021). PubMed: 33976158
- Fan F et al. MicroRNA-744-5p inhibits glioblastoma malignancy by suppressing replication factor C subunit 2. Oncol Lett 22:608 (2021). PubMed: 34188710
- Hu T et al. RFC2, a direct target of miR-744, modulates the cell cycle and promotes the proliferation of CRC cells. J Cell Physiol 235:8319-8333 (2020). PubMed: 32239691
- Guenat D et al. DNA damage response defect in Williams-Beuren syndrome. Int J Mol Med 39:622-628 (2017). WB . PubMed: 28098859