AB3853

Anti-RFC1抗体

Name: RFC1抗体 (ab3853)| Abcam中文官网
Brand: Abcam Limited
SKU: AB3853
Price: 4845 CNY
Availability: InStock
Rating: 3 (1 reviews)

Anti-RFC1 antibody

(1 Review)

(5 Publications)

Rabbit Polyclonal RFC1 antibody. Suitable for WB and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human RFC1.

查看别名

RFC140, RFC1, Replication factor C subunit 1, Activator 1 140 kDa subunit, Activator 1 large subunit, Activator 1 subunit 1, DNA-binding protein PO-GA, Replication factor C 140 kDa subunit, Replication factor C large subunit, A1 140 kDa subunit, RF-C 140 kDa subunit

2 Images

Supplier Data

Western blot - Anti-RFC1 antibody (AB3853)

All lanes:

Western blot - Anti-RFC1 antibody (ab3853) at 0.4 µg/mL

Lane 1:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 50 µg

Lane 2:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 15 µg

Lane 3:

HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Predicted band size: 128 kDa

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Exposure time: 3min

CiteAb

Western blot - Anti-RFC1 antibody (AB3853)

RFC1 western blot using anti-RFC1 antibody ab3853. Publication image and figure legend from Castaño, E., Caviedes, L., et al., 2017, PLoS One, PubMed 28103309.

ab3853 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab3853 please see the product overview.

Relative expression (mRNA) and protein levels of RFC in BP and CP of human placentas.A) RFC mRNA was determined by RT-qPCR in relation to the geometric mean expression of 3 housekeeping genes (GAPDH, YWHAZ and β-actin) and expressed as median and 25-75th percentiles. #p = 0.035 vs. term newborns, Mann Whitney test. B) Western blots using β-actin as calibrator and Caco2 cells as positive control. C) Western blot signals were determined by densitometry and expressed as median and 25-75th percentiles. RFC : reduced folate carrier; BP : basal plate; CP : chorionic plate.

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关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

反应种属

Human

应用

applications

免疫原

Synthetic Peptide within Human RFC1. The exact immunogen used to generate this antibody is proprietary information.

P35251

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Immunogen

存储溶液

pH: 7 - 8 Preservative: 0.1% Sodium azide Constituents: PBS, 1.815% Tris, 1.764% Sodium citrate

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The Reduced Folate Carrier 1 (RFC1) also known as SLC19A1 is a membrane protein that facilitates the transport of reduced folates and antifolate drugs into the cell. It exhibits a mass of approximately 65 kDa. RFC1 functions as an integral part of the cell membrane predominantly expressed in epithelial tissues including the intestine and placenta. It acts as a folate transporter that enables cellular uptake of folates critical for DNA synthesis and repair nucleotide biosynthesis and methylation reactions.

Biological function summary

RFC1 plays a major role in maintaining intracellular concentrations of folates a necessity for cell division and growth. It is part of a broader folate transport system ensuring proper utilization of folates in metabolic processes. The protein functions alongside the Proton-Coupled Folate Transporter (PCFT) and the Folate Receptor (FR) which work in tandem yet accommodate different pH levels and folate forms.

Pathways

RFC1 is essential to the folate metabolic pathway ensuring the movement of 5-methyltetrahydrofolate a critical form of folate across cell membranes. It engages in interactions with proteins like methionine synthase which play a role in homocysteine remethylation to methionine. This pathway is indispensable to methyl group transfer reactions impacting genome stability and gene expression.

Dysfunction in RFC1 activity associates with conditions such as hereditary folate malabsorption and increased susceptibility to certain cancers. Its impaired function can lead to low folate uptake causing severe consequences in rapidly dividing cells. Evidence indicates that alterations in RFC1 activity can influence the effectiveness of antifolate chemotherapeutic agents involving proteins like thymidylate synthase that depend on folate availability.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins PCNA and activator 1. This subunit binds to the primer-template junction. Binds the PO-B transcription element as well as other GA rich DNA sequences. Could play a role in DNA transcription regulation as well as DNA replication and/or repair. Can bind single- or double-stranded DNA.. Interacts with C-terminus of PCNA. 5' phosphate residue is required for binding of the N-terminal DNA-binding domain to duplex DNA, suggesting a role in recognition of non-primer template DNA structures during replication and/or repair.

See full target information RFC1

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文献 (5)

Recent publications for all applications. Explore the full list and refine your search

iScience 28:112219 PubMed40224004

2025

Supinoxin blocks small cell lung cancer progression by inhibiting mitochondrial respiration through DDX5.

Applications

Unspecified application

Species

Unspecified reactive species

Subhadeep Das,Maria P Zea,Matthew P Russon,Zheng Xing,Sandra Torregrosa-Allen,Heidi E Cervantes,Haley Anne Harper,Bennett D Elzey,Elizabeth J Tran

Cell reports 28:3497-3509.e4 PubMed31553917

2019

Functional Analysis of the Replication Fork Proteome Identifies BET Proteins as PCNA Regulators.

Applications

Unspecified application

Species

Unspecified reactive species

Sarah R Wessel,Kareem N Mohni,Jessica W Luzwick,Huzefa Dungrawala,David Cortez

PloS one 12:e0170389 PubMed28103309

2017

Folate Transporters in Placentas from Preterm Newborns and Their Relation to Cord Blood Folate and Vitamin B12 Levels.

Applications

Species

Unspecified reactive species

Erika Castaño,Lorena Caviedes,Sandra Hirsch,Miguel Llanos,Germán Iñiguez,Ana María Ronco

The Journal of cell biology 200:31-44 PubMed23277426

2013

ATAD5 regulates the lifespan of DNA replication factories by modulating PCNA level on the chromatin.

Applications

Species

Human

Kyoo-young Lee,Haiqing Fu,Mirit I Aladjem,Kyungjae Myung

The Journal of biological chemistry 285:10362-9 PubMed20147293

2010

Human ELG1 regulates the level of ubiquitinated proliferating cell nuclear antigen (PCNA) through Its interactions with PCNA and USP1.

Applications

Unspecified application

Species

Unspecified reactive species

Kyoo-Young Lee,Kailin Yang,Martin A Cohn,Nilabja Sikdar,Alan D D'Andrea,Kyungjae Myung

View all publications

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Anti-RFC1抗体

Anti-RFC1 antibody

Western blot - Anti-RFC1 antibody (AB3853)

Western blot - Anti-RFC1 antibody (AB3853)