Anti-RFC抗体(ab272354)
Key features and details
- Rabbit polyclonal to RFC
- Suitable for: WB
- Reacts with: Mouse, Rat, Human, African green monkey
- Isotype: IgG
概述
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产品名称
Anti-RFC抗体 -
描述
兔多克隆抗体to RFC -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Mouse, Rat, Human, African green monkey -
免疫原
Synthetic peptide corresponding to Human RFC aa 200-300.
Database link: P41440 -
阳性对照
- WB: Rat brain and ovary tissue lysates; mouse brain, testis and ovary lysates; human placenta tissue lysate; HEK-293, PC-3 , 22RV1, K562, NIH/3T3 and COS-7 whole cell lysates.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.025% Sodium azide
Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Sodium phosphate dibasic dihydrate -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab272354于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 65 kDa.
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| 说明 |
|---|
|
WB
Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 65 kDa. |
靶标
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功能
Transporter for the intake of folate. Uptake of folate in human placental choriocarcinoma cells occurs by a novel mechanism called potocytosis which functionally couples three components, namely the folate receptor, the folate transporter, and a V-type H(+)-pump. -
组织特异性
Placenta, liver, and to a much smaller extent, in lung. -
序列相似性
Belongs to the reduced folate carrier (RFC) transporter (TC 2.A.48) family. -
翻译后修饰
Heavily glycosylated. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 6573 Human
- Entrez Gene: 20509 Mouse
- Entrez Gene: 29723 Rat
- Omim: 600424 Human
- SwissProt: P41440 Human
- SwissProt: P41438 Mouse
- SwissProt: Q62866 Rat
- Unigene: 84190 Human
see all -
别名
- CHMD antibody
- FLOT 1 antibody
- FLOT1 antibody
see all
图片
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Western blot - Anti-RFC antibody (ab272354)All lanes : Anti-RFC antibody (ab272354) at 0.5 µg/ml
Lane 1 : Rat brain tissue lysates
Lane 2 : Rat ovary tissue lysates
Lane 3 : Mouse brain tissue lysates
Lane 4 : Mouse testicular tissue lysates
Lane 5 : Mouse ovary tissue lysates
Lane 6 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysates
Lane 7 : Human placenta tissue lysates
Lane 8 : HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysates
Lane 9 : COS-7 (African green monkey kidney fibroblast-like cell line) whole cell lysates
Lane 10 : PC-3 (human prostate adenocarcinoma cell line) whole cell lysates
Lane 11 : 22RV1 (human prostate carcinoma cell line) whole cell lysates
Lane 12 : K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysates
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG-HRP at 1/10000 dilution
Developed using the ECL technique.
Predicted band size: 65 kDa
Observed band size: 65 kDa5-20% SDS-PAGE gel.
Blocked membrane with 5% Non-fat Milk/ TBS for 1.5 hour at room temperature.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (1)
ab272354 被引用在 1 文献中.
- Tearle JLE et al. Targeted Single-cell Isolation of Spontaneously Escaping Live Melanoma Cells for Comparative Transcriptomics. Cancer Res Commun 3:1524-1537 (2023). PubMed: 37575281
