Anti-Rex1抗体(ab28141)
Key features and details
- Rabbit polyclonal to Rex1
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-Rex1抗体
参阅全部 Rex1 一抗 -
描述
兔多克隆抗体to Rex1 -
宿主
Rabbit -
经测试应用
适用于: WB, ICC/IFmore details -
种属反应性
与反应: Mouse, Human -
免疫原
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阳性对照
- Lysate of 293T cells overexpressing Rex1, Mouse Embryonic Stem Cells expressing biotinylated Rex1.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab28141于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (1) |
Use a concentration of 0.5 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 32 kDa).
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| ICC/IF | (1) |
Use a concentration of 1 µg/ml.
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| 说明 |
|---|
|
WB
Use a concentration of 0.5 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 32 kDa). |
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ICC/IF
Use a concentration of 1 µg/ml. |
靶标
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功能
Involved in self-renewal property of ES cells (By similarity). May be involved in transcriptional regulation. -
组织特异性
Expressed in kidney, epidermal keratinocytes, prostate epithelial cells, bronchial and small airway lung epithelial cells (at protein level). Expressed in malignant kidney and several carcinoma cell lines (at protein level). Expressed in embryonic stem cells, kidney, epidermal keratinocytes, prostate epithelial cells, bronchial and small airway lung epithelial cells. Expressed in embryonal carcinomas, seminomas, malignant kidney and several carcinoma cell lines. -
序列相似性
Belongs to the krueppel C2H2-type zinc-finger protein family.
Contains 4 C2H2-type zinc fingers. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 132625 Human
- Entrez Gene: 22702 Mouse
- Omim: 614572 Human
- SwissProt: Q96MM3 Human
- SwissProt: P22227 Mouse
- Unigene: 335787 Human
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别名
- hREX-1 antibody
- Reduced Expression 1 Protein antibody
- Reduced expression protein 1 antibody
see all
图片
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Immunocytochemistry/ Immunofluorescence - Anti-Rex1 antibody (ab28141)ICC/IF image of ab28141 stained mouse embryonic stem cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab28141, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue).
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Western blot - Anti-Rex1 antibody (ab28141)All lanes : Anti-Rex1 antibody (ab28141) at 0.5 µg/ml
Lane 1 : Lysate of 293T cells overexpressing Rex1
Lane 2 : Lysate of Mouse Embryonic Stem Cells expressing biotinylated Rex1
Lane 3 : Negative control for tagged protein - Lysate of Mouse Embryonic Stem Cells (endogenous protein present)
Lanes 4-7 : Lysate of various passages of Mouse Embryonic Stem Cells expressing biotinylated Rex1
Predicted band size: 32 kDa
Observed band size: 32 kDa
Additional bands at: 34 kDa (possible non-specific binding), 36 kDa (possible non-specific binding), 38 kDa (possible tagged protein)
In this western blot Anti-Rex1 antibody ab28141 recognizes a band at 32kDa corresponding to the endogenous Rex1 protein and a band at 38 kDa corresponding to biotinylated Rex1. The identities of the bands at 34 and 36 kDa are unknown. It is possible that they represent non-specific binding, but it may be that they represent modified forms of the non-biotinylated endogenous Rex1. The biotinylated Rex1 protein contains an N-terminal FLAG and a 25 AA peptide substrate for biotinylation. Therefore, the biotinylated protein is expected to migrate at a slightly higher molecular weight than endogenous Rex1. The large bands at the top of the image are likely to represent non-specific binding. -
Western blot - Anti-Rex1 antibody (ab28141)Anti-Rex1 antibody (ab28141) at 1 µg/ml + 293T Cells Overexpressing Mouse Rex1 at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 32 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted?
Additional bands at: 100 kDa, 75 kDa. We are unsure as to the identity of these extra bands.
The REX1 protein in these 293T cells contains an N-terminal FLAG tag. We expect the tagged protein to migrate at a higher molecular weight than endogenous REX1.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (15)
ab28141 被引用在 15 文献中.
- Junyent S et al. Wnt- and glutamate-receptors orchestrate stem cell dynamics and asymmetric cell division. Elife 10:N/A (2021). PubMed: 34028355
- Luo H et al. Offspring production of ovarian organoids derived from spermatogonial stem cells by defined factors with chromatin reorganization. J Adv Res 33:81-98 (2021). PubMed: 34603780
- Bustos F et al. Functional Diversification of SRSF Protein Kinase to Control Ubiquitin-Dependent Neurodevelopmental Signaling. Dev Cell 55:629-647.e7 (2020). PubMed: 33080171
- Liu J et al. Deriving rabbit embryonic stem cells by small molecule inhibitors. Am J Transl Res 11:5122-5133 (2019). PubMed: 31497228
- Bustos F et al. RNF12 X-Linked Intellectual Disability Mutations Disrupt E3 Ligase Activity and Neural Differentiation. Cell Rep 23:1599-1611 (2018). PubMed: 29742418