Anti-REV1抗体(ab5088)
Key features and details
- Goat polyclonal to REV1
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG2c
概述
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产品名称
Anti-REV1抗体
参阅全部 REV1 一抗 -
描述
山羊多克隆抗体to REV1 -
宿主
Goat -
经测试应用
适用于: IHC-Pmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Cow, Dog
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免疫原
Synthetic peptide corresponding to Human REV1 aa 1200 to the C-terminus.
Database link: Q9UBZ9-1 -
阳性对照
- IHC-P: Human Testis tissue.
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常规说明
REV1L is the human homolog of the S. cerevisiae mutagenesis protein Rev1. The REV1 protein is conserved from yeast to humans. All REV1 proteins contain a BRCT domain, which is important in protein-protein interactions.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Primary antibody说明
REV1L is the human homolog of the S. cerevisiae mutagenesis protein Rev1. The REV1 protein is conserved from yeast to humans. All REV1 proteins contain a BRCT domain, which is important in protein-protein interactions. -
克隆
多克隆 -
同种型
IgG2c -
研究领域
相关产品
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Compatible Secondaries
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Positive Controls
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
Use a concentration of 4 - 6 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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| 说明 |
|---|
|
IHC-P
Use a concentration of 4 - 6 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
靶标
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功能
Deoxycytidyl transferase involved in DNA repair. Transfers a dCMP residue from dCTP to the 3'-end of a DNA primer in a template-dependent reaction. May assist in the first step in the bypass of abasic lesions by the insertion of a nucleotide opposite the lesion. Required for normal induction of mutations by physical and chemical agents. -
组织特异性
Ubiquitous. -
序列相似性
Belongs to the DNA polymerase type-Y family.
Contains 1 BRCT domain.
Contains 1 umuC domain. -
结构域
The C-terminal domain is necessary for protein interactions. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 51455 Human
- Entrez Gene: 56210 Mouse
- Entrez Gene: 316344 Rat
- GenBank: NP_057400 Human
- Omim: 606134 Human
- SwissProt: Q9UBZ9 Human
- SwissProt: Q920Q2 Mouse
- Unigene: 443077 Human
see all -
别名
- AIBP 80 antibody
- AIBP80 antibody
- Alpha integrin binding protein 80 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-REV1 antibody (ab5088)In paraffin embedded Human Testis shows nuclear staining in spermatogonia and spermatocytes. ab5088 used at 4ug/ml following Steamed antigen retrieval with Tris/EDTA buffer pH 9 visualised using HRP staining.
数据表及文件
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Datasheet download
文献 (3)
ab5088 被引用在 3 文献中.
- Goehringer N et al. Improved Anticancer Activities of a New Pentafluorothio-Substituted Vorinostat-Type Histone Deacetylase Inhibitor. Pharmaceuticals (Basel) 14:N/A (2021). PubMed: 34959719
- Yeo C et al. Epigallocatechin-3-Gallate Suppresses Vasculogenic Mimicry through Inhibiting the Twist/VE-Cadherin/AKT Pathway in Human Prostate Cancer PC-3 Cells. Int J Mol Sci 21:N/A (2020). PubMed: 31936664
- Imani S et al. Prognostic Value of EMT-inducing Transcription Factors (EMT-TFs) in Metastatic Breast Cancer: A Systematic Review and Meta-analysis. Sci Rep 6:28587 (2016). PubMed: 27335258
