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Epigenetics and Nuclear Signaling DNA / RNA DNA Damage & Repair DNA Damage Response BRCT Domain Proteins

Anti-REV1抗体(ab5088)

  • Datasheet
  • SDS
Reviews (1)Q&A (1)References (3)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-REV1 antibody (ab5088)

    Key features and details

    • Goat polyclonal to REV1
    • Suitable for: IHC-P
    • Reacts with: Human
    • Isotype: IgG2c

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    二抗
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    Donkey Anti-Goat IgG H&L (HRP) (ab97110)

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    概述

    • 产品名称

      Anti-REV1抗体
      参阅全部 REV1 一抗
    • 描述

      山羊多克隆抗体to REV1
    • 宿主

      Goat
    • 经测试应用

      适用于: IHC-Pmore details
    • 种属反应性

      与反应: Human
      预测可用于: Mouse, Rat, Cow, Dog
    • 免疫原

      Synthetic peptide corresponding to Human REV1 aa 1200 to the C-terminus.
      Database link: Q9UBZ9-1

      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
    • 阳性对照

      • IHC-P: Human Testis tissue.
    • 常规说明


      REV1L is the human homolog of the S. cerevisiae mutagenesis protein Rev1. The REV1 protein is conserved from yeast to humans. All REV1 proteins contain a BRCT domain, which is important in protein-protein interactions.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    性能

    • 形式

      Liquid
    • 存放说明

      Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
    • 存储溶液

      pH: 7.3
      Preservative: 0.02% Sodium azide
      Constituents: Tris buffered saline, 0.5% BSA
    • Concentration information loading...
    • 纯度

      Immunogen affinity purified
    • 纯化说明

      Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    • Primary antibody说明

      REV1L is the human homolog of the S. cerevisiae mutagenesis protein Rev1. The REV1 protein is conserved from yeast to humans. All REV1 proteins contain a BRCT domain, which is important in protein-protein interactions.
    • 克隆

      多克隆
    • 同种型

      IgG2c
    • 研究领域

      • Epigenetics and Nuclear Signaling
      • DNA / RNA
      • DNA Damage & Repair
      • DNA Damage Response
      • BRCT Domain Proteins
      • Epigenetics and Nuclear Signaling
      • DNA / RNA
      • DNA Synthesis
      • Translesion Synthesis

    相关产品

    • Compatible Secondaries

      • Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)
      • Donkey Anti-Goat IgG H&L (HRP) (ab205723)

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab5088于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    IHC-P
    Use a concentration of 4 - 6 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
    说明
    IHC-P
    Use a concentration of 4 - 6 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    靶标

    • 功能

      Deoxycytidyl transferase involved in DNA repair. Transfers a dCMP residue from dCTP to the 3'-end of a DNA primer in a template-dependent reaction. May assist in the first step in the bypass of abasic lesions by the insertion of a nucleotide opposite the lesion. Required for normal induction of mutations by physical and chemical agents.
    • 组织特异性

      Ubiquitous.
    • 序列相似性

      Belongs to the DNA polymerase type-Y family.
      Contains 1 BRCT domain.
      Contains 1 umuC domain.
    • 结构域

      The C-terminal domain is necessary for protein interactions.
    • 细胞定位

      Nucleus.
    • Target information above from: UniProt accession Q9UBZ9 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • 数据库链接

      • Entrez Gene: 51455 Human
      • Entrez Gene: 56210 Mouse
      • Entrez Gene: 316344 Rat
      • GenBank: NP_057400 Human
      • Omim: 606134 Human
      • SwissProt: Q9UBZ9 Human
      • SwissProt: Q920Q2 Mouse
      • Unigene: 443077 Human
      • Unigene: 260943 Mouse
      • Unigene: 389103 Mouse
      see all
    • 别名

      • AIBP 80 antibody
      • AIBP80 antibody
      • Alpha integrin binding protein 80 antibody
      • Alpha integrin-binding protein 80 antibody
      • DNA repair protein REV 1 antibody
      • DNA repair protein REV1 antibody
      • FLJ21523 antibody
      • MGC163283 antibody
      • MGC26225 antibody
      • REV 1 (yeast homolog) like antibody
      • REV 1 antibody
      • REV 1 homolog antibody
      • REV 1 like antibody
      • REV 1 protein antibody
      • REV 1L antibody
      • REV1 (yeast homolog) like antibody
      • REV1 antibody
      • REV1 homolog (S. cerevisiae) antibody
      • REV1 homolog antibody
      • REV1 like (yeast) antibody
      • REV1 like antibody
      • Rev1 like terminal deoxycytidyl transferase antibody
      • REV1 protein antibody
      • Rev1-like terminal deoxycytidyl transferase antibody
      • REV1_HUMAN antibody
      • REV1L antibody
      see all

    图片

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-REV1 antibody (ab5088)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-REV1 antibody (ab5088)
      In paraffin embedded Human Testis shows nuclear staining in spermatogonia and spermatocytes. ab5088 used at 4ug/ml following Steamed antigen retrieval with Tris/EDTA buffer pH 9 visualised using HRP staining.

    实验方案

    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    数据表及文件

    • SDS download

    • Datasheet download

      Download

    文献 (3)

    发表研究结果有使用 ab5088?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab5088 被引用在 3 文献中.

    • Goehringer N  et al. Improved Anticancer Activities of a New Pentafluorothio-Substituted Vorinostat-Type Histone Deacetylase Inhibitor. Pharmaceuticals (Basel) 14:N/A (2021). PubMed: 34959719
    • Yeo C  et al. Epigallocatechin-3-Gallate Suppresses Vasculogenic Mimicry through Inhibiting the Twist/VE-Cadherin/AKT Pathway in Human Prostate Cancer PC-3 Cells. Int J Mol Sci 21:N/A (2020). PubMed: 31936664
    • Imani S  et al. Prognostic Value of EMT-inducing Transcription Factors (EMT-TFs) in Metastatic Breast Cancer: A Systematic Review and Meta-analysis. Sci Rep 6:28587 (2016). PubMed: 27335258

    客户评价及客户问答

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    1-2 of 2 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-REV1 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (U2OS)
    Permeabilization
    Yes - NP-40
    Specification
    U2OS
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 May 16 2016

    Question

    We have recently expressed recombinant Rev1 protein with a V5 tag. We are interested in an antibody that will recognize untagged Rev1. I have seen on the web that you sell such an antibody but that it has not been tested in a western .... I am not so interested in paying $ 200 for that, then we may think about making our own from the full length protein we have. I was wondering if we could work out a discount or that we can test the antibody for you?

    Read More

    Abcam community

    Verified customer

    Asked on May 20 2005

    Answer

    Thank you for your email. Ab5088 is a Fast-Track antibody and has not yet been fully characterized. We are unable to provide a discount, but we will award $300 of Conference Travel Money to the first researcher who sends us positive feedback (including an image and details of materials & methods). Conclusive negative feedback that leads us to withdraw the antibody will also be rewarded with $300 of Conference Travel Money. Other useful positive and negative feedback will be rewarded with a $15 (£10/€15) Amazon gift certificate. All awards are subject to approval by Abcam scientists and provision of suitable data. Please submit feedback to fast-track@abcam.com

    Read More

    Abcam Scientific Support

    回复于 May 20 2005

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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