重组Anti-Retinoic Acid Receptor alpha抗体[EPR23871-287] - BSA and Azide free (Capture) (ab281211)
![Sandwich ELISA - Anti-Retinoic Acid Receptor alpha antibody [EPR23871-287] - BSA and Azide free (Capture) (ab281211)](https://www.abcam.cn/ps/products/281/ab281211/Images/ab281211-2-human-retinoic-acid-receptor-alpha-elisa-kit-rara-sandwich-elisa-standard-curve-4.jpg "Sandwich ELISA - Anti-Retinoic Acid Receptor alpha antibody [EPR23871-287] - BSA and Azide free (Capture) (ab281211)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23871-287] to Retinoic Acid Receptor alpha - BSA and Azide free (Capture)
- Suitable for: Sandwich ELISA
- Reacts with: Human
概述
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产品名称
Anti-Retinoic Acid Receptor alpha抗体[EPR23871-287] - BSA and Azide free (Capture)
参阅全部 Retinoic Acid Receptor alpha 一抗 -
描述
兔单克隆抗体[EPR23871-287] to Retinoic Acid Receptor alpha - BSA and Azide free (Capture) -
宿主
Rabbit -
经测试应用
适用于: Sandwich ELISAmore details -
种属反应性
与反应: Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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常规说明
ab281211 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human RARA. The recommended pair for sandwich ELISA is:
Capture: ab281211, Human RARA Capture Antibody (unconjugated)
Detector: ab281061, Human RARA Detector Antibody (unconjugated)
The reference range value is 62.5 - 4000 pg/mL.Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
Constituent: 100% PBS -
无载体
是 -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR23871-287 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Conjugation kits
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sELISA pair antibody
- PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
- Streptavidin Conjugation Kit - Lightning-Link® (ab102921)
- Biotinylation Kit / Biotin Conjugation Kit (Fast, Type A) - Lightning-Link® (ab201795)
- Anti-Retinoic Acid Receptor alpha antibody [EPR23871-76] - BSA and Azide free (Detector) (ab281061)
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab281211于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918) and Streptavidin Conjugation Kit - Lightning-Link® (ab102921) and Biotinylation Kit / Biotin Conjugation Kit (Fast and Type A) - Lightning-Link® (ab201795) and Rabbit monoclonal [EPR23871-76] to Retinoic Acid Receptor alpha - BSA and Azide free (Detector) (ab281061).
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| 说明 |
|---|
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Sandwich ELISA
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918) and Streptavidin Conjugation Kit - Lightning-Link® (ab102921) and Biotinylation Kit / Biotin Conjugation Kit (Fast and Type A) - Lightning-Link® (ab201795) and Rabbit monoclonal [EPR23871-76] to Retinoic Acid Receptor alpha - BSA and Azide free (Detector) (ab281061). |
靶标
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功能
Receptor for retinoic acid. Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes. The RXR/RAR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. In the absence of ligand, the RXR-RAR heterodimers associate with a multiprotein complex containing transcription corepressors that induce histone acetylation, chromatin condensation and transcriptional suppression. On ligand binding, the corepressors dissociate from the receptors and associate with the coactivators leading to transcriptional activation. RARA plays an essential role in the regulation of retinoic acid-induced germ cell development during spermatogenesis. Has a role in the survival of early spermatocytes at the beginning prophase of meiosis. In Sertoli cells, may promote the survival and development of early meiotic prophase spermatocytes. In concert with RARG, required for skeletal growth, matrix homeostasis and growth plate function (By similarity). Regulates expression of target genes in a ligand-dependent manner by recruiting chromatin complexes containing MLL5. Mediates retinoic acid-induced granulopoiesis. -
疾病相关
Note=Chromosomal aberrations involving RARA are commonly found in acute promyelocytic leukemia. Translocation t(11;17)(q32;q21) with ZBTB16/PLZF; translocation t(15;17)(q21;q21) with PML; translocation t(5;17)(q32;q11) with NPM. The PML-RARA oncoprotein requires both the PML ring structure and coiled-coil domain for both interaction with UBE2I, nuclear microspeckle location and sumoylation. In addition, the coiled-coil domain functions in blocking RA-mediated transactivation and cell differentiation. -
序列相似性
Belongs to the nuclear hormone receptor family. NR1 subfamily.
Contains 1 nuclear receptor DNA-binding domain. -
结构域
Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain. -
翻译后修饰
Phosphorylated on serine and threonine residues. Phosphorylation does not change during cell cycle. Phosphorylation on Ser-77 is crucial for transcriptional activity (By similarity). Phosphorylation by AKT1 is required for the repressor activity but has no effect on DNA binding, protein stability nor subcellular localization. Phosporylated by PKA in vitro. This phosphorylation on Ser-219 and Ser-369 is critical for ligand binding, nuclear localization and transcriptional activity in response to FSH signaling.
Sumoylated by SUMO2, mainly on Lys-399 which is also required for SENP6 binding. On all-trans retinoic acid (ATRA) binding, a confromational change may occur that allows sumoylation on two additional site, Lys-166 and Lys-171. Probably desumoylated by SENP6. Sumoylation levels determine nuclear localization and regulate ATRA-mediated transcriptional activity.
Trimethylation enhances heterodimerization with RXRA and positively modulates the transcriptional activation.
Ubiquitinated. -
细胞定位
Nucleus. Cytoplasm. Nuclear localization depends on ligand binding, phosphorylation and sumoylation. Transloaction to the nucleus in the absence of ligand is dependent on activation of PKC and the downstream MAPK phosphorylation. - Information by UniProt
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数据库链接
- Entrez Gene: 5914 Human
- Omim: 180240 Human
- SwissProt: P10276 Human
- Unigene: 654583 Human
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别名
- NR1B1 antibody
- Nuclear mitotic apparatus protein retinoic acid receptor alpha fusion protein antibody
- Nuclear receptor subfamily 1 group B member 1 antibody
see all
图片
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Sandwich ELISA - Anti-Retinoic Acid Receptor alpha antibody [EPR23871-287] - BSA and Azide free (Capture) (ab281211)Example of Human Retinoic Acid Receptor alpha standard curve. Background-subtracted data values (mean +/- SD) are graphed.
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![Anti-Retinoic Acid Receptor alpha antibody [EPR23871-287] - BSA and Azide free (Capture) (ab281211)](https://www.abcam.cn/ps/products/281/ab281211/Images/ab281211-1-RecombinantAntibodyBenefitfigure.png)
Anti-Retinoic Acid Receptor alpha antibody [EPR23871-287] - BSA and Azide free (Capture) (ab281211)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (0)
ab281211 尚未被引用在任何文献中。
