Anti-Reticulon 1A抗体[MON162] (ab9274)
![Immunocytochemistry/ Immunofluorescence - Anti-Reticulon 1A antibody [MON162] (ab9274)](https://www.abcam.cn/ps/products/9/ab9274/Images/ab9274-16882-anti-reticulon-1a-antibody-mon162-immunofluorescence.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Reticulon 1A antibody [MON162] (ab9274)")
Key features and details
- Mouse monoclonal [MON162] to Reticulon 1A
- Suitable for: ICC/IF, IHC-Fr
- Reacts with: Human, Pig
- Isotype: IgG1
概述
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产品名称
Anti-Reticulon 1A抗体[MON162]
参阅全部 Reticulon 1A 一抗 -
描述
小鼠单克隆抗体[MON162] to Reticulon 1A -
宿主
Mouse -
特异性
This antibody exclusively recognizes the 135 kD Reticulon-1A protein in immunoblots of NCI-H82 and other SCLC cell lines, and stains normal and pathological neural and neuroendocrine tissues -
经测试应用
适用于: ICC/IF, IHC-Frmore details -
种属反应性
与反应: Human, Pig -
免疫原
Recombinant fragment within Reticulon 1A aa 1-800. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. Partially purified bacterially expressed Reticulon-1A (NSP-A) hybrid protein (beta-GAL-NSP-A 6-776).
Database link: Q16799 -
表位
The epitope of this antibody is located between amino acid residues 338-422 of Reticulon-1A. -
常规说明
A central structure in the secretory pathway of the cell comprises an elaborate endomembranous system with the multifunctional endoplasmic reticulum (ER) as a major constituent. Newly synthesized secretory proteins and a variety of resident proteins of the pathway enter the rough endoplasmic reticulum. Subsequently, the proteins may be sorted selectively to different membrane-delimited organelles including the Golgi apparatus, endosomes, lysosomes and secretory vesicles. Abcam provides antibodies to a new family of ER-associated proteins, the reticulons, which show a tissue specific distribution.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading... -
纯度
Protein G purified -
Primary antibody说明
A central structure in the secretory pathway of the cell comprises an elaborate endomembranous system with the multifunctional endoplasmic reticulum (ER) as a major constituent. Newly synthesized secretory proteins and a variety of resident proteins of the pathway enter the rough endoplasmic reticulum. Subsequently, the proteins may be sorted selectively to different membrane-delimited organelles including the Golgi apparatus, endosomes, lysosomes and secretory vesicles. Abcam provides antibodies to a new family of ER-associated proteins, the reticulons, which show a tissue specific distribution. -
克隆
单克隆 -
克隆编号
MON162 -
骨髓瘤
unknown -
同种型
IgG1 -
研究领域
相关产品
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Compatible Secondaries
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Conjugation kits
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Isotype control
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
Use at an assay dependent concentration.
|
|
| IHC-Fr |
Use at an assay dependent concentration.
|
| 说明 |
|---|
|
ICC/IF
Use at an assay dependent concentration. |
|
IHC-Fr
Use at an assay dependent concentration. |
靶标
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相关性
Reticulon 1A may be involved in neuroendocrine secretion or in membrane trafficking in neuroendocrine cells. It is expressed in neural and neuroendocrine tissues and cell cultures derived therefrom. Expression of isoform RTN1-C is strongly correlated with neuronal differentiation. Different isoforms have been described as a result of alternative splicing. -
细胞定位
Endoplasmic reticulum -
数据库链接
- Entrez Gene: 6252 Human
- Omim: 600865 Human
- SwissProt: Q16799 Human
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别名
- Neuroendocrine specific protein antibody
- NSP antibody
- Reticulon1 antibody
- RTN1 antibody
图片
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Immunocytochemistry/ Immunofluorescence - Anti-Reticulon 1A antibody [MON162] (ab9274)
Immunostaining of NCI-H82 (a human cell line) variant small cell lung cancer
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![Immunohistochemistry (Frozen sections) - Anti-Reticulon 1A antibody [MON162] (ab9274)](https://www.abcam.cn/ps/products/9/ab9274/Images/ab9274-16881-anti-reticulon-1a-antibody-mon162-immunohistochemistry.jpg)
Immunohistochemistry (Frozen sections) - Anti-Reticulon 1A antibody [MON162] (ab9274)Immunohistochemistry on frozen section of swine brain
实验方案
数据表及文件
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Datasheet download
文献 (9)
ab9274 被引用在 9 文献中.
- Qin T et al. Tumor necrosis factor superfamily 15 promotes lymphatic metastasis via upregulation of vascular endothelial growth factor-C in a mouse model of lung cancer. Cancer Sci 109:2469-2478 (2018). PubMed: 29890027
- McGonigle TA et al. Reticulon-1 and Reduced Migration toward Chemoattractants by Macrophages Differentiated from the Bone Marrow of Ultraviolet-Irradiated and Ultraviolet-Chimeric Mice. J Immunol 200:260-270 (2018). PubMed: 29167231
- Grumati P et al. Full length RTN3 regulates turnover of tubular endoplasmic reticulum via selective autophagy. Elife 6:N/A (2017). WB . PubMed: 28617241
- Xu W et al. Normal fibroblasts induce E-cadherin loss and increase lymph node metastasis in gastric cancer. PLoS One 9:e97306 (2014). PubMed: 24845259
- Hens J et al. Neuronal differentiation is accompanied by NSP-C expression. Cell Tissue Res 292:229-37 (1998). PubMed: 9560466
- Senden NH et al. A comparison of NSP-reticulons with conventional neuroendocrine markers in immunophenotyping of lung cancers. J Pathol 182:13-21 (1997). PubMed: 9227337
- van de Velde HJ et al. NSP-encoded reticulons are neuroendocrine markers of a novel category in human lung cancer diagnosis. Cancer Res 54:4769-76 (1994). PubMed: 8062278
- van de Velde HJ et al. NSP-encoded reticulons, neuroendocrine proteins of a novel gene family associated with membranes of the endoplasmic reticulum. J Cell Sci 107 ( Pt 9):2403-16 (1994). PubMed: 7844160
- van de Velde HJ et al. Molecular analysis of expression in rat brain of NSP-A, a novel neuroendocrine-specific protein of the endoplasmic reticulum. Brain Res Mol Brain Res 23:81-92 (1994). PubMed: 7518032
