Anti-Reticulon 1A抗体[MON160] (ab8957)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Reticulon 1A antibody [MON160] (ab8957)](https://www.abcam.cn/ps/products/8/ab8957/Images/ab8957_1.gif "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Reticulon 1A antibody [MON160] (ab8957)")
Key features and details
- Mouse monoclonal [MON160] to Reticulon 1A
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG1
概述
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产品名称
Anti-Reticulon 1A抗体[MON160]
参阅全部 Reticulon 1A 一抗 -
描述
小鼠单克隆抗体[MON160] to Reticulon 1A -
宿主
Mouse -
特异性
This antibody exclusively recognizes the 135 kDa Reticulon-1A protein in immunoblots of NCI-H82 and other SCLC cell lines, and stains normal and pathological neural and neuroendocrine tissues -
经测试应用
适用于: IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Recombinant fragment within Reticulon 1A aa 1-800. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. Partially purified bacterially expressed Reticulon-1A (NSP-A) hybrid protein (beta-GAL-NSP-A 6-776).
Database link: Q16799 -
表位
This antibody is reactive with residues 174-337 of Reticulon-1A. -
常规说明
A central structure in the secretory pathway of the cell comprises an elaborate endomembranous system with the multifunctional endoplasmic reticulum (ER) as a major constituent. Newly synthesized secretory proteins and a variety of resident proteins of the pathway enter the rough endoplasmic reticulum. Subsequently, the proteins may be sorted selectively to different membrane-delimited organelles including the Golgi apparatus, endosomes, lysosomes and secretory vesicles. Abcam provides antibodies to a new family of ER-associated proteins, the reticulons, which show a tissue specific distribution.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading... -
纯度
Protein G purified -
Primary antibody说明
A central structure in the secretory pathway of the cell comprises an elaborate endomembranous system with the multifunctional endoplasmic reticulum (ER) as a major constituent. Newly synthesized secretory proteins and a variety of resident proteins of the pathway enter the rough endoplasmic reticulum. Subsequently, the proteins may be sorted selectively to different membrane-delimited organelles including the Golgi apparatus, endosomes, lysosomes and secretory vesicles. Abcam provides antibodies to a new family of ER-associated proteins, the reticulons, which show a tissue specific distribution. -
克隆
单克隆 -
克隆编号
MON160 -
骨髓瘤
unknown -
同种型
IgG1 -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
Use at an assay dependent concentration.
Recommended range is 1/50 - 1/100 for immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent. |
| 说明 |
|---|
|
IHC-P
Use at an assay dependent concentration. Recommended range is 1/50 - 1/100 for immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent. |
靶标
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相关性
Reticulon 1A may be involved in neuroendocrine secretion or in membrane trafficking in neuroendocrine cells. It is expressed in neural and neuroendocrine tissues and cell cultures derived therefrom. Expression of isoform RTN1-C is strongly correlated with neuronal differentiation. Different isoforms have been described as a result of alternative splicing. -
细胞定位
Endoplasmic reticulum -
数据库链接
- Entrez Gene: 6252 Human
- Omim: 600865 Human
- SwissProt: Q16799 Human
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别名
- Neuroendocrine specific protein antibody
- NSP antibody
- Reticulon1 antibody
- RTN1 antibody
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Reticulon 1A antibody [MON160] (ab8957)
NSP-A immunostaining in human cerebellum using ab8957.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Reticulon 1A antibody [MON160] (ab8957)](https://www.abcam.cn/ps/products/8/ab8957/Images/ab8957_2.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Reticulon 1A antibody [MON160] (ab8957)Human carcinoid (A) and small cell lung carcinoma (B) in paraffin sections, immunostained for NSP-A with ab8957.
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![Schematic Diagram - Anti-Reticulon 1A antibody [MON160] (ab8957)](https://www.abcam.cn/ps/products/8/ab8957/Images/ab8957_3.gif)
Schematic Diagram - Anti-Reticulon 1A antibody [MON160] (ab8957)Schematic representation of of the deduced domain structure of the three NSP-gene encoded proteins, NSP-A, NSP-B and NSP-C and mapping of the epitopes recognized by the different NSP monoclonal antibodies.
实验方案
数据表及文件
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Datasheet download
文献 (3)
ab8957 被引用在 3 文献中.
- Fan Y et al. Inhibition of Reticulon-1A-Mediated Endoplasmic Reticulum Stress in Early AKI Attenuates Renal Fibrosis Development. J Am Soc Nephrol 28:2007-2021 (2017). PubMed: 28137829
- Xiao W et al. Knockdown of RTN1A attenuates ER stress and kidney injury in albumin overload-induced nephropathy. Am J Physiol Renal Physiol 310:F409-15 (2016). PubMed: 26739891
- Fan Y et al. RTN1 mediates progression of kidney disease by inducing ER stress. Nat Commun 6:7841 (2015). PubMed: 26227493
