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AB134100

Anti-Ret 抗体 [EPR2871]

Anti-Ret antibody [EPR2871]

4

(8 Reviews)

|

(65 Publications)

Anti-Ret antibody [EPR2871] (ab134100) is a rabbit monoclonal antibody detecting Ret in Western Blot, IP, IHC-P, ICC/IF, ELISA. Suitable for Human, Mouse, Rat.

- Biophysical QC for unrivalled batch-batch consistency
- Over 50 publications

查看别名

CDHF12, CDHR16, PTC, RET, RET51, Proto-oncogene tyrosine-protein kinase receptor Ret, Cadherin family member 12, Proto-oncogene c-Ret

23 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ret antibody [EPR2871] (AB134100)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ret antibody [EPR2871] (AB134100)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human thyroid gland carcinoma tissue labelling Ret with unpurified ab134100 at a dilution of 1/250.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-Ret antibody [EPR2871] (AB134100)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Ret antibody [EPR2871] (AB134100)

Immunocytochemistry/Immunofluorescence analysis of TT (human thyroid carcinoma epithelial cell) cells labelling Ret with purified ab134100 at a dilution of 1/200. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (1/200) was used as a counter stain.

-ve control : Caco-2 (PMID : 10811228) cells stained with primary antibody (1/100) and secondary antibody, ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ret antibody [EPR2871] (AB134100)
  • IHC-P

PubMed

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ret antibody [EPR2871] (AB134100)

Immunostaining of the Ret proto-oncogene in human Medullary thyroid carcinoma (MTC) samples from GTCAC haplotype carriers & non-carriers.

Two representative slices of Ret Immunostaining in a sample carrier S836S/3'UTR (GTCAC haplotype) (left) and non-carrier of this haplotype (right)

Image from Ceolin L et al., PLoS One. 2016;11(2):e0147840. Fig 4.; doi: 10.1371/journal.pone.0147840. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ret antibody [EPR2871] (AB134100)
  • IHC-P

AbReview62366****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ret antibody [EPR2871] (AB134100)

Formaldehyde-fixed, paraffin-embedded human colon tissue stained for Ret using ab134100 at 1/80 dilution in immunohistochemical analysis, followed by Goat anti-Rabbit IgG (HRP antibody.

This image is courtesy of an anonymous Abreview.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ret antibody [EPR2871] (AB134100)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ret antibody [EPR2871] (AB134100)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human gastric carcinoma tissue labelling Ret with purified ab134100 at a dilution of 1/50. Heat mediated antigen retrieval was performed using EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ret antibody [EPR2871] (AB134100)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ret antibody [EPR2871] (AB134100)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse colon tissue labelling Ret with unpurified ab134100 at a dilution of 1/50 followed by HRP-conjugated goat anti-rabbit IgG (H&L) (ab97051, 1/500). Counter stained with hematoxylin.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-Ret antibody [EPR2871] (AB134100)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Ret antibody [EPR2871] (AB134100)

Immunocytochemistry/Immunofluorescence analysis of Neuro-2a cells labelling Ret with purified ab134100 at a dilution of 1/100. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000) were also used.

Control 1 : primary antibody (1/100) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000).

Control 2 : ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000).

Immunoprecipitation - Anti-Ret antibody [EPR2871] (AB134100)
  • IP

Lab

Immunoprecipitation - Anti-Ret antibody [EPR2871] (AB134100)

ab134100 (purified) at 1/40 immunoprecipitating Ret in Neuro-2a mMouse neuroblastoma cell line) whole cell lysate.

Lane 1 (input) : Neuro-2a whole cell lysate (10μg)

Lane 2 (+) : ab134100 + Neuro-2a whole cell lysate.

Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab134100 in Neuro-2a whole cell lysate.

For western blotting, ab134100 was used at 1/1000 followed by a HRP-conjugated anti-rabbit IgG (specific to the non-reduced form of IgG, 1/1500).

Blocking buffer and dilution concentration : 5% NFDM/TBST.

We are unsure about the nature of 40kDa to 60kDa bands, they might be the intracellular fragments of Ret.

All lanes:

Immunoprecipitation - Anti-Ret antibody [EPR2871] (ab134100)

Predicted band size: 124 kDa

false

Exposure time: 30s

Western blot - Anti-Ret antibody [EPR2871] (AB134100)
  • WB

Supplier Data

Western blot - Anti-Ret antibody [EPR2871] (AB134100)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

LC-2/ad cells express the constitutively active CCDC6-RET fusion. The down-regulation of Ret phospho Y1015 is induced by ponatinib treatment (PMID : 33795352) .

The molecular weight observed is consistent with what has been described in the literature (PMID : 33795352; PMID : 23578175).

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

In Western blot, Anti-Ret antibody (ab134100) staining at 1/1000 dilution.

All lanes:

Western blot - Anti-Ret (phospho Y1015) antibody [EPR26919-93] (<a href='/products/primary-antibodies/ret-phospho-y1015-antibody-epr26919-93-ab324978'>ab324978</a>) at 1/1000 dilution

Lanes 1 and 3:

Untreated LC-2/ad (human Lung Epithelial) whole cell lysate at 50 µg

Lanes 2 and 4:

LC-2/ad treated with 300nM Ponatinib for 2h whole cell lysate at 50 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 60 kDa,36 kDa

false

Exposure time: 180s

Western blot - Anti-Ret antibody [EPR2871] (AB134100)
  • WB

Supplier Data

Western blot - Anti-Ret antibody [EPR2871] (AB134100)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

In Western blot, Anti-Ret antibody (ab134100) staining at 1/1000 dilution.

All lanes:

Western blot - Anti-Ret (phospho Y1015) antibody [EPR26919-93] (<a href='/products/primary-antibodies/ret-phospho-y1015-antibody-epr26919-93-ab324978'>ab324978</a>) at 1/1000 dilution

Lanes 1 and 3:

Untreated TT (human thyroid carcinoma epithelial cell) whole cell lysate at 50 µg

Lanes 2 and 4:

TT treated with 100uM pervanadate for 10 min whole cell lysate at 50 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 150 kDa,36 kDa

false

Exposure time: 37s

Western blot - Anti-Ret antibody [EPR2871] (AB134100)
  • WB

Lab

Western blot - Anti-Ret antibody [EPR2871] (AB134100)

Blocking and dilution buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-Ret antibody [EPR2871] (ab134100) at 1/5000 dilution

All lanes:

SH-SY5Y (human neuroblastoma cell line from bone marrow) whole cell lysate at 10 µg

Secondary

All lanes:

Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 124 kDa

Observed band size: 155 kDa,175 kDa

false

Western blot - Anti-Ret antibody [EPR2871] (AB134100)
  • WB

Supplier Data

Western blot - Anti-Ret antibody [EPR2871] (AB134100)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

In Western blot, Anti-Ret antibody [EPR2871] (ab134100) staining at 1/1000 dilution.

All lanes:

Western blot - Anti-Ret (phospho Y1096) antibody [EPR26918-23] (<a href='/products/primary-antibodies/ret-phospho-y1096-antibody-epr26918-23-ab322346'>ab322346</a>) at 1/1000 dilution

Lane 1:

Untreated TT (human thyroid carcinoma epithelial cell) whole cell lysate (untreated membrane) at 20 µg

Lane 2:

TT treated with 1mM pervanadate for 30min whole cell lysate (untreated membrane) at 20 µg

Lane 3:

Untreated TT whole cell lysate (Alkaline phosphatase treated membrane) at 20 µg

Lane 4:

TT treated with 1mM pervanadate for 30min whole cell lysate (Alkaline phosphatase treated membrane) at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 175 kDa,36 kDa

false

Exposure time: 180s

Western blot - Anti-Ret antibody [EPR2871] (AB134100)
  • WB

Supplier Data

Western blot - Anti-Ret antibody [EPR2871] (AB134100)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The up-regulation of Ret phospho Y1096 is induced by GDNF treatment (PMID : 12091387).

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

In Western blot, Anti-Ret antibody [EPR2871] (ab134100) staining at 1/1000 dilution.

All lanes:

Western blot - Anti-Ret (phospho Y1096) antibody [EPR26918-23] (<a href='/products/primary-antibodies/ret-phospho-y1096-antibody-epr26918-23-ab322346'>ab322346</a>) at 1/1000 dilution

Lane 1:

Untreated TT (human thyroid carcinoma epithelial cell) whole cell lysate at 40 µg

Lane 2:

TT treated with 100ng/ml GDNF for 10 mintues whole cell lysate at 40 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 175 kDa,36 kDa

false

Exposure time: 180s

Western blot - Anti-Ret antibody [EPR2871] (AB134100)
  • WB

Supplier Data

Western blot - Anti-Ret antibody [EPR2871] (AB134100)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

In Western blot, Anti-Ret antibody [EPR2871] (ab134100) staining at 1/1000 dilution.

All lanes:

Western blot - Anti-Ret (phospho Y905) antibody [EPR26917-10] (<a href='/products/primary-antibodies/ret-phospho-y905-antibody-epr26917-10-ab320078'>ab320078</a>) at 1/1000 dilution

Lane 1:

Untreated TT (human thyroid carcinoma epithelial cell) whole cell lysate (untreated membrane) at 20 µg

Lane 2:

TT treated with 1mM pervanadate for 30min whole cell lysate (untreated membrane) at 20 µg

Lane 3:

Untreated TT whole cell lysate (Alkaline phosphatase treated membrane) at 20 µg

Lane 4:

TT treated with 1mM pervanadate for 30min whole cell lysate (Alkaline phosphatase treated membrane) at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 175 kDa,36 kDa

false

Exposure time: 180s

Western blot - Anti-Ret antibody [EPR2871] (AB134100)
  • WB

Supplier Data

Western blot - Anti-Ret antibody [EPR2871] (AB134100)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The up-regulation of Ret phospho Y905 is induced by GDNF treatment (PMID : 33921066).

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

In Western blot, Anti-Ret antibody [EPR2871] (ab134100) staining at 1/1000 dilution.

All lanes:

Western blot - Anti-Ret (phospho Y905) antibody [EPR26917-10] (<a href='/products/primary-antibodies/ret-phospho-y905-antibody-epr26917-10-ab320078'>ab320078</a>) at 1/1000 dilution

Lane 1:

Untreated TT (human thyroid carcinoma epithelial cell) whole cell lysate at 40 µg

Lane 2:

TT treated with 100ng/ml GDNF for 10 mintues whole cell lysate at 40 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 175 kDa,36 kDa

false

Exposure time: 180s

Western blot - Anti-Ret antibody [EPR2871] (AB134100)
  • WB

Lab

Western blot - Anti-Ret antibody [EPR2871] (AB134100)

Blocking and dilution buffer : 5% NFDM /TBST.

We are unsure about the nature of 40kDa to 60kDa bands, they might be the intracellular fragments of Ret.

All lanes:

Western blot - Anti-Ret antibody [EPR2871] (ab134100) at 1/10000 dilution

All lanes:

Neuro-2a (mouse neuroblastoma cell line) whole cell lysate at 10 µg

Secondary

All lanes:

Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/2000 dilution

Predicted band size: 124 kDa

Observed band size: 155 kDa,175 kDa

false

Exposure time: 3min

ELISA - Anti-Ret antibody [EPR2871] (AB134100)
  • ELISA

Lab

ELISA - Anti-Ret antibody [EPR2871] (AB134100)

ELISA antigen dose-response curve using purified ab134100 at 0-1000 ng/ml. Antigen concentration of 1000 ng/mL. An Alkaline-Phosphatase-conjugated goat anti-rabbit IgG (H+L) (1/2500) was used as the secondary antibody.

Western blot - Anti-Ret antibody [EPR2871] (AB134100)
  • WB

Supplier Data

Western blot - Anti-Ret antibody [EPR2871] (AB134100)

Blocking and Diluting buffer and concentration : 5% NFDM/TBST

All lanes:

Western blot - Anti-Ret antibody [EPR2871] (ab134100) at 1/10000 dilution

Lane 1:

Mouse brain tissue lysate at 20 µg

Lane 2:

Rat brain tissue lysate at 20 µg

Secondary

All lanes:

Peroxidase-conjugated goat anti-rabbit IgG, (H+L) at 1/1000 dilution

Predicted band size: 124 kDa,13 kDa

Observed band size: 155 kDa,20 kDa

false

Exposure time: 3min

Western blot - Anti-Ret antibody [EPR2871] (AB134100)
  • WB

Supplier Data

Western blot - Anti-Ret antibody [EPR2871] (AB134100)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) staining at 1/5000 dilution.

In Western blot, Anti-Ret antibody (ab134100) staining at 1/1000 dilution.

All lanes:

Western blot - Anti-Ret (phospho Y1015) antibody [EPR26919-93] (<a href='/products/primary-antibodies/ret-phospho-y1015-antibody-epr26919-93-ab324978'>ab324978</a>) at 1/1000 dilution

Lane 1:

293T cells transfected with an empty vector containing a his-tag, whole cell lysate at 10 µg

Lane 2:

293T cells transfected with a human wild-type Ret expression vector containing a his-tag, whole cell lysate at 10 µg

Lane 3:

293T cells transfected with a human Ret (Y1015F mutant) expression vector containing a his-tag, whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 150 kDa,36 kDa

false

Exposure time: 37s

Western blot - Anti-Ret antibody [EPR2871] (AB134100)
  • WB

Lab

Western blot - Anti-Ret antibody [EPR2871] (AB134100)

Blocking and dilution buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-Ret antibody [EPR2871] (ab134100) at 1/1000 dilution

All lanes:

TT (human thyroid carcinoma cell line) whole cell lysate at 10 µg

Secondary

All lanes:

Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 124 kDa

Observed band size: 155 kDa,175 kDa

false

Western blot - Anti-Ret antibody [EPR2871] (AB134100)
  • WB

Lab

Western blot - Anti-Ret antibody [EPR2871] (AB134100)

Blocking and dilution buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-Ret antibody [EPR2871] (ab134100) at 1/1000 dilution

Lane 1:

Mouse brain tissue at 20 µg

Lane 2:

Rat brain tissue at 20 µg

Secondary

All lanes:

Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 124 kDa

Observed band size: 155 kDa,175 kDa

false

Western blot - Anti-Ret antibody [EPR2871] (AB134100)
  • WB

Unknown

Western blot - Anti-Ret antibody [EPR2871] (AB134100)

All lanes:

Western blot - Anti-Ret antibody [EPR2871] (ab134100) at 1/10000 dilution

Lane 1:

Neuro-2a (mouse neuroblastoma cell line) cell lysate at 10 µg

Lane 2:

TT (human thyroid carcinoma cell line) cell lysate at 10 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

Predicted band size: 124 kDa

false

Western blot - Anti-Ret antibody [EPR2871] (AB134100)
  • WB

CiteAb

Western blot - Anti-Ret antibody [EPR2871] (AB134100)

Ret western blot using anti-Ret antibody [EPR2871] ab134100. Publication image and figure legend from Nakaoku, T., Kohno, T., et al., 2018, Nat Commun, PubMed 29434222.

ab134100 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab134100 please see the product overview.

Resistance to vandetanib by RET-S904F mutation. a Immunoblot analysis of the wild type and S904F mutant CCDC6-RET fusion proteins. (Left) An expression vector encoding full-length wild type or S904F mutant CCDC6-RET cDNA was transiently introduced into H1299 lung cancer cells. After exposure to the indicated concentrations of vandetanib for 6 h, phosphorylation at tyrosines 905 and 1015 were detected. The signal intensities, calculated as the ratio of phosphorylated to total CCDC6-RET fusion proteins, are shown at the bottom. (Right) The experiment (shown on the left) was performed separately three times (Supplementary Fig. 8). The graph shows the mean ratios of phospho-Ret (pTyr905) to total RET from three separate experiments with standard deviations (shown as error bars). Concentrations showing statistical significance (p < 0.05 by t-test) are marked with an asterisk. ACTB : beta-actin. b Cell growth assay using IL3-independent Ba/F3 cells carrying lentivirally transduced CCDC6-RET cDNA with or without the S904F mutation. Ba/F3 cells (2000 cells) were plated in quadruplicate in 96-well plates and treated with serially diluted vandetanib. After incubation for 72 h, cell viability was measured using the CellTiter-Glo luminescent reagent with EnVision and the viability curves with standard deviations (shown as error bars) were calculated with GraphPad Prism version 6.0. Reproducibility was confirmed by performing the same experiment three times. c In vitro kinase assays. Recombinant RET kinase domain (KD) (amino acids 658–1072) with or without the S904F mutation was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag (gene accession number : NM_020630). The assay was initiated by addition of 32P-ATP to the reaction mixture and serially diluted vandetanib, and the IGF1Rtide synthetic peptide was added as the substrate (KKKSPGEYVNIEFG). After incubation at 30 °C for 20 min, the radioactivity was measured in a TriLux scintillation counter after transferring the products onto a phosphocellulose P81 plate. IC50 values with 95% confidence interval were calculated using GraphPad Prism version 6.0. Error bars show standard deviations. Reproducibility was confirmed by performing the same experiment three times

false

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR2871

亚型

IgG

不含载体蛋白

No

反应种属

Mouse, Rat, Human

应用

ELISA, ICC/IF, IHC-P, WB, IP

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

style
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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50", "IHCP-species-notes": "<p><strong>For unpurified use at 1/50 - 1/500.</strong></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/100", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50", "IHCP-species-notes": "<p><strong>For unpurified use at 1/50 - 1/500.</strong></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/100", "ICCIF-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Synthetic peptide": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ELISA-species-checked": "testedAndGuaranteed", "ELISA-species-dilution-info": "0.5 µg/mL", "ELISA-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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产品详情

What is this antibody validated in?
Anti-Ret antibody [EPR2871] (ab134100) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF), ELISA in Human, Mouse, Rat samples.

What is the molecular weight of Ret?
Anti-Ret [EPR2871] (ab134100) specifically detects a band for Ret (UniProt: P07949) at a molecular weight of 124kDa.

Trusted by the scientific community
Anti-Ret [EPR2871] (ab134100) was first used in a scientific publication in 2012 and has been cited over 50 times in peer-reviewed journals.

Reviewed by scientists
Anti-Ret [EPR2871] (ab134100) has over 5 independent reviews from customers.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Other related products
We have a range of other formats of antibody clone [EPR2871] also available for your convenience: ab134100, Carrier free - ab214791, Alexa Fluor® 488 - ab237105, Alexa Fluor® 647 - ab311074, Alexa Fluor® 594 - ab311662, Alexa Fluor® 568 - ab312936, Alexa Fluor® 555 - ab313148, Alexa Fluor® 750 - ab321074

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Shipping conditions update: ambient shipping

This product will be delivered at ambient temperature instead of chilled – this is by design. Extensive stability testing confirmed that our products are suitable for shipment under ambient conditions and maintain expected quality.

Why the change?

It’s part of our commitment to more sustainable packaging solutions, with ambient deliveries using eco-friendly materials such as recyclable cardboard instead of polystyrene.

What you need to know

  • Ambient shipments come with a flyer explaining the below.
  • No ice will be included in ambient shipments, but mixed orders (ambient and cold-chain items) will still arrive with ice packs to protect temperature-sensitive products.
  • Warranty coverage remains fully valid, aligned with our validated shipping method.
  • Please store the product as per the datasheet instructions upon receipt.

Find out more - https://www.abcam.com/en-us/support/shipping-storage-support/ambient-shipping

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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A
存储溶液
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
运输条件
Conditional Ambient
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Stable for 12 months at -20°C
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Ret also known as "rearranged during transfection" is a receptor tyrosine kinase with a mass of approximately 170-175 kDa. Scientists often study Ret because of its key role in cellular signaling pathways. It is primarily expressed in neural crest-derived cells and tissues including the kidneys and the enteric nervous system. Ret is also found in various other cell types throughout the body such as endocrine and hematopoietic tissues.
Biological function summary

The Ret protein facilitates several critical processes within the body. It functions as part of a complex with co-receptors called GFRα (GDNF family receptor alpha) which are required for binding ligands such as GDNF (glial cell line-derived neurotrophic factor). This binding activates downstream signaling pathways that influence cell growth differentiation and survival particularly within the nervous system. The proper functioning of Ret is essential for the development and maintenance of these cellular environments.

Pathways

Ret is an important component of the MAPK/ERK and PI3K/AKT signaling pathways. These pathways mediate various cellular responses including cell proliferation survival and apoptosis. The interactions of Ret with other proteins like SHC and GRB2 within these pathways create ripple effects that impact larger cellular networks. Through these pathways Ret connects with multiple proteins to modulate essential biological processes.

Ret's dysregulation is closely associated with multiple endocrine neoplasia type 2 (MEN2) and Hirschsprung's disease. These conditions arise due to mutations affecting Ret's signaling capabilities. In MEN2 Ret mutations lead to uncontrolled cell growth often resulting in medullary thyroid carcinoma. Hirschsprung's disease on the other hand involves a failure in neural crest cell migration leading to congenital gut motility issues. Researchers explore Ret's interactions with other proteins such as endothelin receptor B (EDNRB) to further understand these diseases.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Receptor tyrosine-protein kinase involved in numerous cellular mechanisms including cell proliferation, neuronal navigation, cell migration, and cell differentiation in response to glia cell line-derived growth family factors (GDNF, NRTN, ARTN, PSPN and GDF15) (PubMed : 20064382, PubMed : 20616503, PubMed : 20702524, PubMed : 21357690, PubMed : 21454698, PubMed : 24560924, PubMed : 28846097, PubMed : 28846099, PubMed : 28953886, PubMed : 31118272). In contrast to most receptor tyrosine kinases, RET requires not only its cognate ligands but also coreceptors, for activation (PubMed : 21994944, PubMed : 23333276, PubMed : 28846097, PubMed : 28846099, PubMed : 28953886). GDNF ligands (GDNF, NRTN, ARTN, PSPN and GDF15) first bind their corresponding GDNFR coreceptors (GFRA1, GFRA2, GFRA3, GFRA4 and GFRAL, respectively), triggering RET autophosphorylation and activation, leading to activation of downstream signaling pathways, including the MAPK- and AKT-signaling pathways (PubMed : 21994944, PubMed : 23333276, PubMed : 24560924, PubMed : 25242331, PubMed : 28846097, PubMed : 28846099, PubMed : 28953886). Acts as a dependence receptor via the GDNF-GFRA1 signaling : in the presence of the ligand GDNF in somatotrophs within pituitary, promotes survival and down regulates growth hormone (GH) production, but triggers apoptosis in absence of GDNF (PubMed : 20616503, PubMed : 21994944). Required for the molecular mechanisms orchestration during intestine organogenesis via the ARTN-GFRA3 signaling : involved in the development of enteric nervous system and renal organogenesis during embryonic life, and promotes the formation of Peyer's patch-like structures, a major component of the gut-associated lymphoid tissue (By similarity). Mediates, through interaction with GDF15-receptor GFRAL, GDF15-induced cell-signaling in the brainstem which triggers an aversive response, characterized by nausea, vomiting, and/or loss of appetite in response to various stresses (PubMed : 28846097, PubMed : 28846099, PubMed : 28953886). Modulates cell adhesion via its cleavage by caspase in sympathetic neurons and mediates cell migration in an integrin (e.g. ITGB1 and ITGB3)-dependent manner (PubMed : 20702524, PubMed : 21357690). Also active in the absence of ligand, triggering apoptosis through a mechanism that requires receptor intracellular caspase cleavage (PubMed : 21357690). Triggers the differentiation of rapidly adapting (RA) mechanoreceptors (PubMed : 20064382). Involved in the development of the neural crest (By similarity). Regulates nociceptor survival and size (By similarity). Phosphorylates PTK2/FAK1 (PubMed : 21454698).. Isoform 1. Isoform 1 in complex with GFRAL induces higher activation of MAPK-signaling pathway than isoform 2 in complex with GFRAL.
See full target information RET
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文献 (65)

Recent publications for all applications. Explore the full list and refine your search

Genome medicine 17:102 PubMed40999499

2025

EMB is essential for enteric nervous system development mediated by PI3K signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Zhi Li,Didi Zhuansun,Xinyao Meng,Heying Yang,Jun Xiao,Yingjian Chen,Jing Wang,Xiaosi Yu,Zejian Li,Jingyi You,Xuyong Chen,Chenzhao Feng,Luyao Wu,Xufeng Chu,Weicheng Duan,Kang Wang,Zongzhe Li,Jinfa Tou,Lei Yu,Weibing Tang,Yuanmei Liu,Xuewu Jiang,Hongxia Ren,Mei Yu,Qiang Yin,Xiang Liu,Zhilin Xu,Dianming Wu,Chunlei Jiao,Donghai Yu,Xiaojuan Wu,Tianqi Zhu,Jixin Yang,Lei Xiang,Jing Wang,Qiong Wang,Bingyan Zhou,Di Wang,Ke Chen,Handan Mao,Bin Wang,Jianghua Zhan,Cong-Yi Wang,Wanjiang Zeng,Feng Chen,Bo Xiong,Jiexiong Feng

American journal of cancer research 15:452-469 PubMed40084368

2025

GDF15 promotes the resistance of epithelial ovarian cancer cells to gemcitabine via DHCR24-mediated cholesterol metabolism to elevate ABCB1 and ABCC1 levels in lipid rafts.

Applications

Unspecified application

Species

Unspecified reactive species

Linlin Guo,Yan He,Huang Xin

Molecular oncology 19:614-634 PubMed39825568

2025

Consensus molecular subtyping of colorectal carcinoma brain metastases reveals a metabolic signature associated with poor patient survival.

Applications

Unspecified application

Species

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Barnabas Irmer,Darius Wlochowitz,Carolin Krekeler,Katharina Maria Richter,Suganja Chandrabalan,Michaela Bayerlova,Alexander Wolff,Georg Lenz,Lena-Christin Conradi,Hans-Ulrich Schildhaus,Christine Stadelmann,Veit Rohde,Martin Proescholdt,Gabriela Salinas,Kia Homayounfar,Tanja Kuhlmann,Stephan Hailfinger,Tobias Pukrop,Kerstin Menck,Tim Beissbarth,Annalen Bleckmann

Cell reports. Medicine 5:101559 PubMed38744275

2024

The adipose-neural axis is involved in epicardial adipose tissue-related cardiac arrhythmias.

Applications

Unspecified application

Species

Unspecified reactive species

Yubao Fan,Shanshan Huang,Suhua Li,Bingyuan Wu,Qi Zhao,Li Huang,Zhenda Zheng,Xujing Xie,Jia Liu,Weijun Huang,Jiaqi Sun,Xiulong Zhu,Jieming Zhu,Andy Peng Xiang,Weiqiang Li

eLife 12: PubMed38407266

2024

Molecular classification and tumor microenvironment characteristics in pheochromocytomas.

Applications

Unspecified application

Species

Unspecified reactive species

Sen Qin,Yawei Xu,Shimiao Yu,Wencong Han,Shiheng Fan,Wenxiang Ai,Kenan Zhang,Yizhou Wang,Xuehong Zhou,Qi Shen,Kan Gong,Luyang Sun,Zheng Zhang

Biomolecules 14: PubMed38254638

2024

GDF-15 Inhibits ADP-Induced Human Platelet Aggregation through the GFRAL/RET Signaling Complex.

Applications

Unspecified application

Species

Unspecified reactive species

Baikang Xie,Wenjing Tang,Shuang Wen,Fen Chen,Chao Yang,Min Wang,Yong Yang,Wei Liang

BJC reports 1:13 PubMed39516358

2023

Fulvestrant plus vandetanib versus placebo for the treatment of patients with metastatic breast cancer resistant to aromatase inhibitor therapy (FURVA): a multicentre, Phase 2, randomised controlled trial.

Applications

Unspecified application

Species

Unspecified reactive species

Mark Beresford,Angela Casbard,Zoe Hudson,Margherita Carucci,Kate Ingarfield,Julia Gee,Joanna Smith,Terri Kitson,Fouad Alchami,Tracie-Ann Madden,Larrie Hayward,David Hwang,Saiqa Spensley,Simon Waters,Duncan Wheatley,Robert H Jones

Cell reports 42:113070 PubMed37659079

2023

TMEM127 suppresses tumor development by promoting RET ubiquitination, positioning, and degradation.

Applications

Unspecified application

Species

Unspecified reactive species

Qianjin Guo,Zi-Ming Cheng,Hector Gonzalez-Cantú,Matthew Rotondi,Gabriela Huelgas-Morales,Purushoth Ethiraj,Zhijun Qiu,Jonathan Lefkowitz,Wan Song,Bethany N Landry,Hector Lopez,Cynthia M Estrada-Zuniga,Shivi Goyal,Mohammad Aasif Khan,Timothy J Walker,Exing Wang,Faqian Li,Yanli Ding,Lois M Mulligan,Ricardo C T Aguiar,Patricia L M Dahia

iScience 26:107690 PubMed37680484

2023

Human iPS cell-derived sensory neurons can be infected by SARS-CoV-2.

Applications

Unspecified application

Species

Unspecified reactive species

Anthony Flamier,Punam Bisht,Alexsia Richards,Danielle L Tomasello,Rudolf Jaenisch

Nature communications 14:2378 PubMed37185772

2023

Isthmin-1 (Ism1) modulates renal branching morphogenesis and mesenchyme condensation during early kidney development.

Applications

Unspecified application

Species

Unspecified reactive species

Ge Gao,Xiaoping Li,Zhixin Jiang,Liliana Osorio,Ying Lam Tang,Xueqing Yu,Guoxiang Jin,Zhongjun Zhou
View all publications
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替代产品

Primary Antibodies

AB74154

Anti-Ret (phospho Y1015) antibody

Western blot - Anti-Ret (phospho Y1015) antibody (AB74154)

  • 0
  • 0
Primary Antibodies

AB51103

Anti-Ret (phospho Y1062) antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ret (phospho Y1062) antibody (AB51103)

  • 0
  • 0
Primary Antibodies

AB90119

Anti-Ret antibody [MM0529-10F12]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ret antibody [MM0529-10F12] (AB90119)

  • 0
  • 0
Primary Antibodies

AB184189

Anti-Ret antibody

Western blot - Anti-Ret antibody (AB184189)

  • 0
  • 0

搭配使用产品

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB15580

Anti-Ki67 antibody

BOND RX™ Validated|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody (AB15580)

  • 5
  • 230
Primary Antibodies

AB176564

Anti-GDNF antibody [EPR2714(N)]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-GDNF antibody [EPR2714(N)] (AB176564)

  • 5
  • 3
Primary Antibodies

AB183734

Anti-GFP antibody [EPR14104]

RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFP antibody [EPR14104] (AB183734)

  • 5
  • 24

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