重组Anti-Reptin/TIP49B/RUVB2抗体[EPR4146] (ab108962)
![Western blot - Anti-Reptin/TIP49B/RUVB2 antibody [EPR4146] (ab108962)](https://www.abcam.cn/ps/products/108/ab108962/Images/ReptinTIP49BRUVB2-Primary-antibodies-ab108962-2.JPG "Western blot - Anti-Reptin/TIP49B/RUVB2 antibody [EPR4146] (ab108962)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4146] to Reptin/TIP49B/RUVB2
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-Reptin/TIP49B/RUVB2抗体[EPR4146]
参阅全部 Reptin/TIP49B/RUVB2 一抗 -
描述
兔单克隆抗体[EPR4146] to Reptin/TIP49B/RUVB2 -
宿主
Rabbit -
经测试应用
适用于: WBmore details
不适用于: Flow Cyt,ICC/IF,IHC-P or IP -
种属反应性
与反应: Human
预测可用于: Mouse
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- HepG2, Daudi, HeLa, and SKBR-3 cell lysates
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
EPR4146 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab108962于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000 - 1/10000. Detects a band of approximately 48 kDa (predicted molecular weight: 51 kDa).
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| 说明 |
|---|
|
WB
1/1000 - 1/10000. Detects a band of approximately 48 kDa (predicted molecular weight: 51 kDa). |
应用说明
Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.
靶标
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功能
Possesses single-stranded DNA-stimulated ATPase and ATP-dependent DNA helicase (5' to 3') activity. Component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. The NuA4 complex ATPase and helicase activities seem to be, at least in part, contributed by the association of RUVBL1 and RUVBL2 with EP400. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. RUVBL2 plays an essential role in oncogenic transformation by MYC and also modulates transcriptional activation by the LEF1/TCF1-CTNNB1 complex. May also inhibit the transcriptional activity of ATF2. -
组织特异性
Ubiquitously expressed. Highly expressed in testis and thymus. -
序列相似性
Belongs to the ruvB family. -
结构域
The C-terminal domain is required for association with ATF2. -
翻译后修饰
Phosphorylated upon DNA damage, probably by ATM or ATR. -
细胞定位
Nucleus matrix. Nucleus > nucleoplasm. Cytoplasm. Membrane. Mainly localized in the nucleus, associated with nuclear matrix or in the nuclear cytosol. Although it is also present in the cytoplasm and associated with the cell membranes. - Information by UniProt
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数据库链接
- Entrez Gene: 10856 Human
- Entrez Gene: 20174 Mouse
- Omim: 604788 Human
- SwissProt: Q9Y230 Human
- SwissProt: Q9WTM5 Mouse
- Unigene: 515846 Human
- Unigene: 34410 Mouse
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别名
- 48 kDa TATA box-binding protein-interacting protein antibody
- 48 kDa TBP-interacting protein antibody
- 48-kDa TATA box-binding protein-interacting protein antibody
see all
图片
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Western blot - Anti-Reptin/TIP49B/RUVB2 antibody [EPR4146] (ab108962)All lanes : Anti-Reptin/TIP49B/RUVB2 antibody [EPR4146] (ab108962) at 1/1000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : Daudi cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : SKBR-3 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 51 kDa -
![Anti-Reptin/TIP49B/RUVB2 antibody [EPR4146] (ab108962)](https://www.abcam.cn/ps/products/108/ab108962/Images/ab108962-2-benefits-of-recombinant-antibodies.png)
Anti-Reptin/TIP49B/RUVB2 antibody [EPR4146] (ab108962)
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab108962 被引用在 1 文献中.
- Satkunanathan S et al. Establishment of a novel cell line for the enhanced production of recombinant adeno-associated virus vectors for gene therapy. Hum Gene Ther 25:929-41 (2014). WB . PubMed: 25072415