AB196027

Anti-Reptin/TIP49B/RUVB2抗体[EP4145(2)]

Name: Reptin/TIP49B/RUVB2抗体[EP4145(2)] (ab196027)| Abcam中文官网
Brand: Abcam Limited
SKU: AB196027
Price: 4599 CNY
Availability: InStock
Rating: 4 (1 reviews)

Anti-Reptin/TIP49B/RUVB2 antibody [EP4145(2)]

RabMAb
了解详情

(1 Review)

(1 Publication)

Rabbit Monoclonal Reptin/TIP49B/RUVB2 antibody. Suitable for WB and reacts with Rat, Human, Mouse samples. Cited in 1 publication.

查看别名

INO80J, TIP48, TIP49B, CGI-46, RUVBL2, RuvB-like 2, 48 kDa TATA box-binding protein-interacting protein, 51 kDa erythrocyte cytosolic protein, INO80 complex subunit J, Repressing pontin 52, TIP49b, TIP60-associated protein 54-beta, 48 kDa TBP-interacting protein, ECP-51, Reptin 52, TAP54-beta

2 Images

Supplier Data

Western blot - Anti-Reptin/TIP49B/RUVB2 antibody [EP4145(2)] (AB196027)

All lanes:

Western blot - Anti-Reptin/TIP49B/RUVB2 antibody [EP4145(2)] (ab196027) at 1/10000 dilution

Lane 1:

HepG2 cell lysate at 20 µg

Lane 2:

Daudi cell lysate at 20 µg

Lane 3:

HeLa cell lysate at 20 µg

Lane 4:

SK-BR-3 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 51 kDa

false

Supplier Data

Western blot - Anti-Reptin/TIP49B/RUVB2 antibody [EP4145(2)] (AB196027)

All lanes:

Western blot - Anti-Reptin/TIP49B/RUVB2 antibody [EP4145(2)] (ab196027) at 1/1000 dilution

Lane 1:

C6 cell lysate at 10 µg

Lane 2:

RAW 264.7 cell lysate at 10 µg

Lane 3:

PC12 cell lysate at 10 µg

Lane 4:

NIH 3T3 cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 51 kDa

false

不同偶联物与剂型 (1)

Carrier free

Anti-Reptin/TIP49B/RUVB2 antibody [EP4145(2)] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EP4145(2)

亚型

IgG

不含载体蛋白

反应种属

Mouse, Rat, Human

应用

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "" } } }

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产品详情

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Reptin also known as TIP49B or RUVB2 is an ATPase with a mass of approximately 52 kDa. It is expressed ubiquitously across various tissues playing significant roles in cellular processes. Mechanically Reptin functions as part of a complex that facilitates chromatin remodeling and transcription regulation. It often works in conjunction with its paralog Pontin/TIP49A/RUVB1 forming a dodecameric ring structure essential for its activity.

Biological function summary

Reptin plays essential roles in modulating gene expression and contributing to the assembly of ribonucleoprotein complexes. It forms part of the chromatin remodeling complexes such as INO80 and TIP60 participating in DNA repair processes and transcriptional regulation. Its function in these complexes makes it indispensable for maintaining genomic integrity and controlling cell cycle progression.

Pathways

Various critical cellular pathways involve Reptin's biological function. It is an important player in the Wnt/beta-catenin signaling pathway where it regulates the transcription of target genes. Additionally Reptin associates with the Myc pathway contributing to oncogenic transformation. In these pathways Reptin interacts with proteins like beta-catenin and c-Myc integrating signals necessary for cell proliferation and differentiation.

Aberrant Reptin activity links to cancer and neurological disorders. Overexpression of Reptin has strong connections to tumor progression and metastasis in cancers including colorectal and lung cancer. In these diseases Reptin partners with proteins such as c-Myc augmenting its transcriptional activity to promote cell growth. Additionally Reptin's dysregulation may influence neurodegenerative conditions by disrupting protein complexes involved in maintaining neural functions.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Possesses single-stranded DNA-stimulated ATPase and ATP-dependent DNA helicase (5' to 3') activity; hexamerization is thought to be critical for ATP hydrolysis and adjacent subunits in the ring-like structure contribute to the ATPase activity (PubMed : 10428817, PubMed : 17157868, PubMed : 33205750). Component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A (PubMed : 14966270). This modification may both alter nucleosome -DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription (PubMed : 14966270). This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair (PubMed : 14966270). The NuA4 complex ATPase and helicase activities seem to be, at least in part, contributed by the association of RUVBL1 and RUVBL2 with EP400 (PubMed : 14966270). NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage (PubMed : 14966270). Component of a SWR1-like complex that specifically mediates the removal of histone H2A.Z/H2AZ1 from the nucleosome (PubMed : 24463511). Proposed core component of the chromatin remodeling INO80 complex which exhibits DNA- and nucleosome-activated ATPase activity and catalyzes ATP-dependent nucleosome sliding (PubMed : 16230350, PubMed : 21303910). Plays an essential role in oncogenic transformation by MYC and also modulates transcriptional activation by the LEF1/TCF1-CTNNB1 complex (PubMed : 10882073, PubMed : 16014379). May also inhibit the transcriptional activity of ATF2 (PubMed : 11713276). Involved in the endoplasmic reticulum (ER)-associated degradation (ERAD) pathway where it negatively regulates expression of ER stress response genes (PubMed : 25652260). May play a role in regulating the composition of the U5 snRNP complex (PubMed : 28561026).

See full target information RUVBL2

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Aging cell 24:e14418 PubMed39610020

2024

Knockdown of RUVBL2 improves hnRNPA2/B1-stress granules dynamics to inhibit perioperative neurocognitive disorders in aged mild cognitive impairment rats.

Applications

Unspecified application

Species

Unspecified reactive species

Zixuan Wang,Chenyi Yang,Xinyi Wang,Huihui Liao,Xing Liu,Huan Liu,Miao Zhang,Lin Zhang,Haiyun Wang

View all publications

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Anti-Reptin/TIP49B/RUVB2抗体[EP4145(2)]