AB10510

Anti-RENT1/hUPF1抗体

Name: RENT1/hUPF1抗体 (ab10510)| Abcam中文官网
Brand: Abcam Limited
SKU: AB10510
Price: 4845 CNY
Availability: InStock
Rating: 5 (2 reviews)

Anti-RENT1/hUPF1 antibody

(2 Reviews)

(3 Publications)

Goat Polyclonal RENT1/hUPF1 antibody. Suitable for WB and reacts with Mouse, Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human UPF1 aa 250-300.

查看别名

KIAA0221, RENT1, UPF1, Regulator of nonsense transcripts 1, ATP-dependent helicase RENT1, Nonsense mRNA reducing factor 1, Up-frameshift suppressor 1 homolog, NORF1, hUpf1

2 Images

Unknown

Western blot - Anti-RENT1/hUPF1 antibody (AB10510)

ab10510 detection of human RENT1 by Western blot.

Samples : Whole cell lysate (30 μg) from lymphoblastoid cell lines (EBV-immotalized B-cell from a normal individual).

Antibody : ab10510 used at 1 μg/ml.

Detection : Chemiluminescence.

All lanes:

Western blot - Anti-RENT1/hUPF1 antibody (ab10510)

Predicted band size: 124 kDa

false

Supplier Data

Western blot - Anti-RENT1/hUPF1 antibody (AB10510)

All lanes:

Western blot - Anti-RENT1/hUPF1 antibody (ab10510) at 0.4 µg/mL

Lane 1:

HeLa whole cell lysate

Lane 2:

NIH/3T3 whole cell lysate

Predicted band size: 124 kDa

false

Exposure time: 30s

关键信息

宿主种属

Goat

克隆

Polyclonal

亚型

IgG

不含载体蛋白

反应种属

Mouse, Human

应用

applications

免疫原

Synthetic Peptide within Human UPF1 aa 250-300. The exact immunogen used to generate this antibody is proprietary information.

Q92900

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/2500", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/2500", "WB-species-notes": "<p></p>" }, "Chicken": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Guinea pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Platypus": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Turkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Xenopus laevis": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Xenopus tropicalis": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Zebrafish": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Immunogen

纯化说明

Antibodies were affinity purified using the peptide immobilized on solid support.

存储溶液

pH: 7 - 8 Preservative: 0.1% Sodium azide Constituents: PBS, 1.815% Tris, 1.764% Sodium citrate

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

RENT1/hUPF1 also known as regulator of nonsense transcripts 1 or human UPF1 is an important factor in RNA surveillance mechanisms. It is an ATP-dependent helicase with a molecular mass of approximately 130 kDa. This protein is ubiquitously expressed in various tissues highlighting its importance across different biological systems. Mechanically UPF1 plays an essential role in the nonsense-mediated mRNA decay (NMD) pathway where it interacts with other core NMD factors to identify and degrade mRNAs containing premature stop codons.

Biological function summary

RENT1/hUPF1 ensures the quality control of mRNA by facilitating the rapid degradation of defective transcripts and thereby preventing the synthesis of potentially harmful truncated proteins. As part of the NMD complex UPF1 partners with proteins like UPF2 and UPF3 to form a surveillance mechanism that maintains cellular homeostasis. The complex cooperates to distinguish transcripts that require elimination from those necessary for normal cellular function.

Pathways

RENT1/hUPF1 integrates into essential cellular pathways beyond just NMD. The protein also links to the mRNA decay and translation initiation pathways playing significant roles alongside proteins such as SMG1 and eRF1. In these pathways UPF1 regulates gene expression by modulating mRNA stability and protein synthesis ensuring that only correctly processed mRNAs translate into proteins.

RENT1/hUPF1 connects to conditions like neurodevelopmental disorders and cancer. Dysregulation of UPF1 activity can lead to improper mRNA decay contributing to the accumulation of faulty transcripts which may drive disease pathogenesis. In particular UPF1 alterations are linked to the disruption of pathways involving key proteins like p53 which are critical in tumor suppression. Understanding UPF1's role in these disorders could present opportunities for therapeutic interventions.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

RNA-dependent helicase required for nonsense-mediated decay (NMD) of aberrant mRNAs containing premature stop codons and modulates the expression level of normal mRNAs (PubMed : 11163187, PubMed : 16086026, PubMed : 18172165, PubMed : 21145460, PubMed : 21419344, PubMed : 24726324). Is recruited to mRNAs upon translation termination and undergoes a cycle of phosphorylation and dephosphorylation; its phosphorylation appears to be a key step in NMD (PubMed : 11544179, PubMed : 25220460). Recruited by release factors to stalled ribosomes together with the SMG1C protein kinase complex to form the transient SURF (SMG1-UPF1-eRF1-eRF3) complex (PubMed : 19417104). In EJC-dependent NMD, the SURF complex associates with the exon junction complex (EJC) (located 50-55 or more nucleotides downstream from the termination codon) through UPF2 and allows the formation of an UPF1-UPF2-UPF3 surveillance complex which is believed to activate NMD (PubMed : 21419344). Phosphorylated UPF1 is recognized by EST1B/SMG5, SMG6 and SMG7 which are thought to provide a link to the mRNA degradation machinery involving exonucleolytic and endonucleolytic pathways, and to serve as adapters to protein phosphatase 2A (PP2A), thereby triggering UPF1 dephosphorylation and allowing the recycling of NMD factors (PubMed : 12554878). UPF1 can also activate NMD without UPF2 or UPF3, and in the absence of the NMD-enhancing downstream EJC indicative for alternative NMD pathways (PubMed : 18447585). Plays a role in replication-dependent histone mRNA degradation at the end of phase S; the function is independent of UPF2 (PubMed : 16086026, PubMed : 18172165). For the recognition of premature termination codons (PTC) and initiation of NMD a competitive interaction between UPF1 and PABPC1 with the ribosome-bound release factors is proposed (PubMed : 18447585, PubMed : 25220460). The ATPase activity of UPF1 is required for disassembly of mRNPs undergoing NMD (PubMed : 21145460). Together with UPF2 and dependent on TDRD6, mediates the degradation of mRNA harboring long 3'UTR by inducing the NMD machinery (By similarity). Also capable of unwinding double-stranded DNA and translocating on single-stranded DNA (PubMed : 30218034).

See full target information UPF1

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文献 (3)

Recent publications for all applications. Explore the full list and refine your search

PLoS genetics 11:e1005422 PubMed26241656

2015

Retrohoming of a Mobile Group II Intron in Human Cells Suggests How Eukaryotes Limit Group II Intron Proliferation.

Applications

Unspecified application

Species

Unspecified reactive species

David M Truong,F Curtis Hewitt,Joseph H Hanson,Xiaoxia Cui,Alan M Lambowitz

PloS one 8:e77711 PubMed24204929

2013

SMG1 identified as a regulator of Parkinson's disease-associated alpha-synuclein through siRNA screening.

Applications

Species

Unspecified reactive species

Adrienne Henderson-Smith,Donald Chow,Bessie Meechoovet,Meraj Aziz,Sandra A Jacobson,Holly A Shill,Marwan N Sabbagh,John N Caviness,Charles H Adler,Erika D Driver-Dunckley,Thomas G Beach,Hongwei Yin,Travis Dunckley

Molecular and cellular biology 28:883-95 PubMed18025108

2007

Caffeine regulates alternative splicing in a subset of cancer-associated genes: a role for SC35.

Applications

Species

Human

Jia Shi,Zhen Hu,Kirk Pabon,Kathleen W Scotto

View all publications

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Anti-RENT1/hUPF1抗体

Anti-RENT1/hUPF1 antibody

Western blot - Anti-RENT1/hUPF1 antibody (AB10510)

Western blot - Anti-RENT1/hUPF1 antibody (AB10510)