重组Anti-Renin抗体[EPR6433] (ab125012)
![Western blot - Anti-Renin antibody [EPR6433] (ab125012)](https://www.abcam.cn/ps/products/125/ab125012/Images/ab125012-139127-anti-renin-antibody-epr6433-western-blot.jpg "Western blot - Anti-Renin antibody [EPR6433] (ab125012)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6433] to Renin
- Suitable for: Flow Cyt (Intra), WB
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-Renin抗体[EPR6433]
参阅全部 Renin 一抗 -
描述
兔单克隆抗体[EPR6433] to Renin -
宿主
Rabbit -
经测试应用
适用于: Flow Cyt (Intra), WBmore details
不适用于: ICC/IF,IHC-P or IP -
种属反应性
与反应: Human
不与反应: Mouse, Rat -
免疫原
Synthetic peptide within Human Renin aa 50-150. The exact sequence is proprietary.
Database link: P00797 -
阳性对照
- K562, 293T, and HepG2 whole cell lysate (ab7900). K562 cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
解离常数（KD）
KD = 1.70 x 10 -11 M Learn more about KD -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR6433 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab125012于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody |
|
| WB |
1/1000 - 1/10000. Detects a band of approximately 47 kDa (predicted molecular weight: 45 kDa).
|
| 说明 |
|---|
|
Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody |
|
WB
1/1000 - 1/10000. Detects a band of approximately 47 kDa (predicted molecular weight: 45 kDa). |
应用说明
Is unsuitable for ICC/IF,IHC-P or IP.
靶标
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功能
Renin is a highly specific endopeptidase, whose only known function is to generate angiotensin I from angiotensinogen in the plasma, initiating a cascade of reactions that produce an elevation of blood pressure and increased sodium retention by the kidney. -
疾病相关
Defects in REN are a cause of renal tubular dysgenesis (RTD) [MIM:267430]. RTD is an autosomal recessive severe disorder of renal tubular development characterized by persistent fetal anuria and perinatal death, probably due to pulmonary hypoplasia from early-onset oligohydramnios (the Potter phenotype).
Defects in REN are the cause of familial juvenile hyperuricemic nephropathy type 2 (HNFJ2) [MIM:613092]. It is a renal disease characterized by juvenile onset of hyperuricemia, slowly progressive renal failure and anemia. -
序列相似性
Belongs to the peptidase A1 family. -
细胞定位
Secreted. Membrane. Associated to membranes via binding to ATP6AP2. - Information by UniProt
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数据库链接
- Entrez Gene: 5972 Human
- Omim: 179820 Human
- SwissProt: P00797 Human
- Unigene: 3210 Human
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别名
- Angiotensin forming enzyme antibody
- Angiotensin forming enzyme precursor antibody
- Angiotensinogenase antibody
see all
图片
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Western blot - Anti-Renin antibody [EPR6433] (ab125012)All lanes : Anti-Renin antibody [EPR6433] (ab125012) at 1/1000 dilution
Lane 1 : K562 lysate
Lane 2 : 293T (Human embryonic kidney epithelial cell) cell lysate
Lane 3 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 45 kDa
Observed band size: 47 kDa why is the actual band size different from the predicted? -
![Flow Cytometry (Intracellular) - Anti-Renin antibody [EPR6433] (ab125012)](https://www.abcam.cn/ps/products/125/ab125012/Images/ab125012-139126-anti-renin-antibody-epr6433-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-Renin antibody [EPR6433] (ab125012)Intracellular flow cytometric analysis of permeabilized K562 cells using ab125012 at 1/10 shown in red or a rabbit IgG negative control shown in green.
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![OI-RD Scanning - Anti-Renin antibody [EPR6433] (ab125012)](https://www.abcam.cn/ps/products/125/ab125012/Images/ab125012-139125-anti-renin-antibody-epr6433.jpg)
OI-RD Scanning - Anti-Renin antibody [EPR6433] (ab125012) -
![Anti-Renin antibody [EPR6433] (ab125012)](https://www.abcam.cn/ps/products/125/ab125012/Images/ab125012-4-benefits-of-recombinant-antibodies.png)
Anti-Renin antibody [EPR6433] (ab125012)
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab125012 尚未被引用在任何文献中。