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AB280998

重组Anti-REG4抗体[EPR22810-281] - BSA and Azide free (Detector)

Anti-REG4 antibody [EPR22810-281] - BSA and Azide free (Detector)

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Rabbit Recombinant Monoclonal REG4 antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

查看别名

GISP, RELP, REG4, Regenerating islet-derived protein 4, REG-4, Gastrointestinal secretory protein, REG-like protein, Regenerating islet-derived protein IV, Reg IV

1 Images
Sandwich ELISA - Anti-REG4 antibody [EPR22810-281] - BSA and Azide free (Detector) (AB280998)
  • sELISA

Lab

Sandwich ELISA - Anti-REG4 antibody [EPR22810-281] - BSA and Azide free (Detector) (AB280998)

Sandwich ELISA of ab253588 with the capture antibody ab281148 dilution at 2 µg/mL and detector antibody ab280998 dilution at 0.5 µg/mL.

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR22810-281

亚型

IgG

不含载体蛋白

Yes

反应种属

Human

应用

sELISA

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }
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产品详情

ab280998 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human REG4. The recommended pair for sandwich ELISA is:
Capture: ab281148, Human REG4 Capture Antibody (unconjugated)
Detector: ab280998, Human REG4 Detector Antibody (unconjugated)
The reference range value is 15.63 - 2000 pg/ml.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A
存储溶液
Constituents: PBS
运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
+4°C
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

REG4 also known as Reg IV or regenerating islet-derived 4 is a protein with a mass of about 19 kDa. It belongs to the regenerating gene family and has roles in cell growth and regeneration. REG4 expression occurs mainly in the gastric antrum small intestine colon and pancreas. It also appears in certain epithelial tissues particularly ones undergoing regeneration or transformation. REG4's structure includes a C-type lectin domain which suggests its potential involvement in cell adhesion or signaling.
Biological function summary

REG4 strengthens the protection of epithelial cells and supports regeneration during tissue damage or inflammation. It acts as part of cell growth and survival pathways and does not form large complexes on its own. The protein can promote proliferation and maintain integrity in epithelial tissues influencing both normal and cancerous cell behavior. The presence of REG4 might modulate cell response to environmental stress and impact cellular differentiation processes.

Pathways

REG4 impacts both the MAPK/ERK and PI3K/AKT signaling pathways. These pathways are important for cell proliferation and survival indicating REG4's involvement in cellular stress response and oncogenesis. REG4 interacts with other proteins such as EGFR (Epidermal Growth Factor Receptor) which participate in these pathways. This protein co-activity may enhance cellular responses contributing to growth regulation and survival signaling in various tissues.

REG4 has a strong association with colorectal cancer and inflammatory bowel disease (IBD). Its overexpression has been noted in colorectal cancer tissues and can relate to tumor growth and metastasis. In the context of IBD REG4 expression connects with inflammation markers and mucosal healing processes. Proteins like MUC2 (Mucin 2) and CDX2 (Caudal Type Homeobox 2) involved in these diseases show relationships with REG4 emphasizing its role in disease progression and regulation within epithelial tissues.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Calcium-independent lectin displaying mannose-binding specificity and able to maintain carbohydrate recognition activity in an acidic environment. May be involved in inflammatory and metaplastic responses of the gastrointestinal epithelium.
See full target information REG4
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搭配使用产品

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