Anti-Ref2P抗体(ab178440)
Key features and details
- Rabbit polyclonal to Ref2P
- Suitable for: WB
- Reacts with: Drosophila melanogaster
- Isotype: IgG
概述
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产品名称
Anti-Ref2P抗体 -
描述
兔多克隆抗体to Ref2P -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Drosophila melanogaster -
免疫原
Synthetic peptide corresponding to Drosophila melanogaster Ref2P aa 200-300 conjugated to keyhole limpet haemocyanin.
Database link: P14199 -
阳性对照
- This antibody gave a positive signal in Atg8a mutant Fruit Flies (D.melanogaster).
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab178440于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB | (1) |
Use at an assay dependent concentration. Predicted molecular weight: 65 kDa.
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说明 |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 65 kDa. |
靶标
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功能
Implicated in sigma rhabdovirus multiplication and necessary for male fertility. -
序列相似性
Contains 1 OPR domain.
Contains 1 UBA domain.
Contains 1 ZZ-type zinc finger. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 35246 Drosophila melanogaster
- SwissProt: P14199 Drosophila melanogaster
- Unigene: 12210 Drosophila melanogaster
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别名
- Protein ref(2)P antibody
- ref(2)P antibody
- REF2P_DROME antibody
- Refractory to sigma P antibody
图片
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All lanes : Anti-Ref2P antibody (ab178440) at 1/500 dilution (Blocking 60 minutes in TBS+0.1% Tween-20 + 5% non-fat milk)
Lane 1 : Wt adult drosophila lysate
Lane 2 : Atg8a mutant adult drosophila lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Anti-rabbit HRP at 1/10000 dilution
Predicted band size: 65 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?
Exposure time: 2 secondsGel 8% acrylamide, transferred onto PVDF membrane for 60 minutes at 100V in 10% ethanol buffer. Anti-Ref2P antibody 1:500 incubated overnight at 4ºC, with gentle agitation in TBS + 0.1% Tween-20.
The genotype of the Atg8a mutant flies used is: Atg8aKG07569
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All lanes : Anti-Ref2P antibody (ab178440) at 1/100 dilution (Milk blocked)
Lanes 1-3 : Atg8a mutant fruit flies (D.melanogaster)
Lanes 4-6 : Fruit flies (D.melanogaster) expressing ubiquitously Ref(2)p RNAi
Predicted band size: 65 kDa
Observed band size: 92 kDa why is the actual band size different from the predicted?The bands observed at the bottom of the gel represent Tubulin and were used as a loading control within this experiment.
数据表及文件
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SDS download
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Datasheet download
文献 (49)
ab178440 被引用在 49 文献中.
- Xue M et al. Loss of Paip1 causes translation reduction and induces apoptotic cell death through ISR activation and Xrp1. Cell Death Discov 9:288 (2023). PubMed: 37543696
- Zhu JY et al. APOL1-G2 accelerates nephrocyte cell death by inhibiting the autophagy pathway. Dis Model Mech 16:N/A (2023). PubMed: 37969018
- Rimal S et al. Reverse electron transfer is activated during aging and contributes to aging and age-related disease. EMBO Rep 24:e55548 (2023). PubMed: 36794623
- Sanchez-Martinez A et al. FBXO7/ntc and USP30 antagonistically set the ubiquitination threshold for basal mitophagy and provide a target for Pink1 phosphorylation in vivo. PLoS Biol 21:e3002244 (2023). PubMed: 37535686
- Pai YL et al. The deubiquitinase Leon/USP5 interacts with Atg1/ULK1 and antagonizes autophagy. Cell Death Dis 14:540 (2023). PubMed: 37607937