Anti-Reelin抗体[G10] (ab78540)
![Immunohistochemistry (PFA fixed) - Anti-Reelin antibody [G10] (ab78540)](https://www.abcam.cn/ps/products/78/ab78540/Images/ab78540-94334-anti-reelin-antibody-g10-immunohistochemistry.jpg "Immunohistochemistry (PFA fixed) - Anti-Reelin antibody [G10] (ab78540)")
Key features and details
- Mouse monoclonal [G10] to Reelin
- Suitable for: WB, IHC (PFA fixed)
- Reacts with: Mouse, Rat, Human
- Isotype: IgG1
概述
-
产品名称
Anti-Reelin抗体[G10]
参阅全部 Reelin 一抗 -
描述
小鼠单克隆抗体[G10] to Reelin -
宿主
Mouse -
经测试应用
适用于: WB, IHC (PFA fixed)more details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Recombinant fragment corresponding to Mouse Reelin aa 150-500.
Database link: Q60841 -
阳性对照
- Mouse liver or kidney. Rat brain lysate and Mouse brain membrane lysate. Mouse embryonic cerebral cortex.
-
常规说明
This product was changed from ascites to tissue culture supernatant on 17 May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.1% Sodium azide
Constituents: 1.45% Sodium chloride, 0.242% Tris -
Concentration information loading... -
纯度
Tissue culture supernatant -
纯化说明
Purified from TCS. -
克隆
单克隆 -
克隆编号
G10 -
同种型
IgG1 -
轻链类型
kappa -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab78540于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use at an assay dependent concentration. Predicted molecular weight: 388 kDa.
|
|
| IHC (PFA fixed) |
Use at an assay dependent concentration.
|
| 说明 |
|---|
|
WB
Use at an assay dependent concentration. Predicted molecular weight: 388 kDa. |
|
IHC (PFA fixed)
Use at an assay dependent concentration. |
靶标
-
功能
Extracellular matrix serine protease that plays a role in layering of neurons in the cerebral cortex and cerebellum. Regulates microtubule function in neurons and neuronal migration. Affects migration of sympathetic preganglionic neurons in the spinal cord, where it seems to act as a barrier to neuronal migration. Enzymatic activity is important for the modulation of cell adhesion. Binding to the extracellular domains of lipoprotein receptors VLDLR and LRP8/APOER2 induces tyrosine phosphorylation of DAB1 and modulation of TAU phosphorylation. -
组织特异性
Abundantly produced during brain ontogenesis by the Cajal-Retzius cells and other pioneer neurons located in the telencephalic marginal zone and by granule cells of the external granular layer of the cerebellum. In adult brain, preferentially expressed in GABAergic interneurons of prefrontal cortices, temporal cortex, hippocampus and glutamatergic granule cells of cerebellum. Expression is reduced to about 50% in patients with schizophrenia. Also expressed in fetal and adult liver. -
疾病相关
Lissencephaly 2 -
序列相似性
Belongs to the reelin family.
Contains 16 BNR repeats.
Contains 8 EGF-like domains.
Contains 1 reelin domain. -
发展阶段
Expressed in fetal and postnatal brain and liver. Expression in postnatal human brain is high in the cerebellum. -
结构域
The basic C-terminal region is essential for secretion. -
细胞定位
Secreted > extracellular space > extracellular matrix. - Information by UniProt
-
数据库链接
- Entrez Gene: 5649 Human
- Entrez Gene: 19699 Mouse
- Entrez Gene: 24718 Rat
- Omim: 600514 Human
- SwissProt: P78509 Human
- SwissProt: Q60841 Mouse
- SwissProt: P58751 Rat
- Unigene: 655654 Human
see all -
别名
- LIS2 antibody
- PRO1598 antibody
- Reeler antibody
see all
图片
-
Immunohistochemistry (PFA fixed) - Anti-Reelin antibody [G10] (ab78540)
ab78540, at 1/1000 dilution staining Reelin in embryonic cerebral cortex at 15 days in utero, with the pial surface above and the ventricle below. The positive zone is the Cajal-Retzius cell layer in the marginal zone.
This image was generated using the ascites version of the product.
-
![Western blot - Anti-Reelin antibody [G10] (ab78540)](https://www.abcam.cn/ps/products/78/ab78540/Images/Reelin-Primary-antibodies-ab78540-7.jpg)
Western blot - Anti-Reelin antibody [G10] (ab78540)All lanes : Anti-Reelin antibody [G10] (ab78540) at 1/500 dilution
Lane 1 : Rat brain lysate
Lane 2 : Mouse brain membrane lysate
Secondary
All lanes : Goat anti-mouse secondary antibody
conjugated to HRP
Predicted band size: 388 kDa
Observed band size: 388 kDa
Additional bands at: 140 kDa. We are unsure as to the identity of these extra bands.This image was generated using the ascites version of the product.
-
![Immunohistochemistry (PFA fixed) - Anti-Reelin antibody [G10] (ab78540)](https://www.abcam.cn/ps/products/78/ab78540/Images/Reelin-Primary-antibodies-ab78540-9.jpg)
Immunohistochemistry (PFA fixed) - Anti-Reelin antibody [G10] (ab78540)Immunolocalization of Reelin (red) in the cortex of e14.5 mouse CNS using ab78540 at 1/1000 dilution. The blue stain is Hoechst nuclear counter stain.
This image was generated using the ascites version of the product.
-
![Western blot - Anti-Reelin antibody [G10] (ab78540)](https://www.abcam.cn/ps/products/78/ab78540/Images/Reelin-Primary-antibodies-ab78540-6.jpg)
Western blot - Anti-Reelin antibody [G10] (ab78540)Anti-Reelin antibody [G10] (ab78540) at 1/500 dilution + Rat brain lysate at 10 µg
Secondary
Goat anti-mouse secondary
antibody conjugated to HRP
Predicted band size: 388 kDa
Observed band size: 388 kDaThis image was generated using the ascites version of the product.
实验方案
数据表及文件
-
SDS download
-
Datasheet download
文献 (36)
ab78540 被引用在 36 文献中.
- Johnson KO et al. Postsynaptic NMDA Receptor Expression Is Required for Visual Corticocollicular Projection Refinement in the Mouse Superior Colliculus. J Neurosci 43:1310-1320 (2023). PubMed: 36717228
- van Bruggen R et al. A Versatile Strategy for Genetic Manipulation of Cajal-Retzius Cells in the Adult Mouse Hippocampus. eNeuro 10:N/A (2023). PubMed: 37775311
- Ardalan M et al. Reelin cells and sex-dependent synaptopathology in autism following postnatal immune activation. Br J Pharmacol 179:4400-4422 (2022). PubMed: 35474185
- Mercier MS et al. Long-term potentiation in neurogliaform interneurons modulates excitation-inhibition balance in the temporoammonic pathway. J Physiol 600:4001-4017 (2022). PubMed: 35876215
- Jacobi A et al. Overlapping transcriptional programs promote survival and axonal regeneration of injured retinal ganglion cells. Neuron 110:2625-2645.e7 (2022). PubMed: 35767994