AB218648

redFluor™ 710 Anti-CD45RA抗体[HI100]

Name: redFluor™ 710 Anti-CD45RA抗体[HI100] (ab218648)| Abcam中文官网
Brand: Abcam Limited
SKU: AB218648
Price: 1584 CNY
Availability: InStock

redFluor™ 710 Anti-CD45RA antibody [HI100]

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(1 Publication)

Mouse Monoclonal CD45 antibody - conjugated to redFluor™ 710. Suitable for Flow Cyt and reacts with Human samples. Cited in 1 publication.

查看别名

CD45, PTPRC, Receptor-type tyrosine-protein phosphatase C, Leukocyte common antigen, T200, L-CA

1 Images

Flow Cytometry - redFluor™ 710 Anti-CD45RA antibody [HI100] (AB218648)

Flow Cyt

Supplier Data

Flow Cytometry - redFluor™ 710 Anti-CD45RA antibody [HI100] (AB218648)

Flow Cytometry analysis of Human peripheral blood lymphocytes labeling CD45RA with ab218648 at 5 μL (0.25 μg) per reaction (solid line) or 0.25 μg redFluor™ 710 Mouse IgG2b isotype control (dashed line).

关键信息

宿主种属

Mouse

克隆

Monoclonal

克隆号

HI100

亚型

IgG2b

轻链类型

kappa

偶联物

redFluor™ 710

激发波长/发射波长

Ex: 684nm, Em: 704nm

不含载体蛋白

反应种属

Human

应用

Flow Cyt

applications

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "5 µL for 10^5-10^8 Cells", "FlowCyt-species-notes": "<p></p>" }, "Chimpanzee": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" } } }

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产品详情

Conjugated to redFluor™ 710. RedFluor™ 710 dye is excited by the red (633-647 nm) laser and has a peak emission of 710 nm. The recommended band pass filter for this dye is 710/50. RedFluor™ 710 can be used as an alternative for Alexa Fluor® 700. Confirm that your cytometer is configured to detect this fluorochrome.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

纯化说明

ab218648 was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.

存储溶液

pH: 7.2 Preservative: 0.09% Sodium azide Constituents: 0.87% Sodium chloride, 0.12% Sodium dihydrogen phosphate, 0.1% Gelatin

运输条件

Blue Ice

储存信息

Do Not Freeze|Store in the dark

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

CD45RA also known as CD45RA antigen and T200 is an isoform of the CD45 protein family. It is a transmembrane protein tyrosine phosphatase with a molecular mass of approximately 220 kDa. CD45RA expression is abundant on naive T-cells and subsets of other immune cells like B cells. Researchers frequently use CD45RA antibodies including CD45RA-PE and CD45RA-FITC for flow cytometry to identify and study immune cell subsets.

Biological function summary

CD45RA plays a significant role in regulating T-cell receptor (TCR) signaling. By dephosphorylating specific phosphotyrosine residues on substrates CD45RA modulates signal transduction pathways important for T-cell activation and differentiation. This protein does not act alone but is part of a regulatory network that influences immune responses and cellular development through its interaction with other cell surface receptors and intracellular kinases.

Pathways

CD45RA modulation is integral to T-cell development and activation processes. It is an important participant in the T-cell receptor signaling pathway and the Jak-STAT signaling pathway. In these pathways CD45 interacts with proteins such as Lck and Fyn which are essential for propagating receptor-mediated signaling events in T-cells. Effective signal regulation through CD45RA is necessary to ensure appropriate immune responses.

CD45RA has links to autoimmune diseases such as multiple sclerosis and rheumatoid arthritis. The aberrant expression or function of CD45RA on immune cells can lead to improper immune activation or suppression contributing to the pathology of these diseases. Crossover with other proteins such as CD45RO which is an isoform involved in memory T-cell function indicates a broader network of immune regulation where CD45RA maintains the balance between immune activation and tolerance.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor (PubMed : 35767951). Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. Dephosphorylates LYN, and thereby modulates LYN activity (By similarity). Interacts with CLEC10A at antigen presenting cell-T cell contact; CLEC10A on immature dendritic cells recognizes Tn antigen-carrying PTPRC/CD45 receptor on effector T cells and modulates T cell activation threshold to limit autoreactivity.. (Microbial infection) Acts as a receptor for human cytomegalovirus protein UL11 and mediates binding of UL11 to T-cells, leading to reduced induction of tyrosine phosphorylation of multiple signaling proteins upon T-cell receptor stimulation and impaired T-cell proliferation.

See full target information PTPRC

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in immunology 16:1598436 PubMed40621452

2025

Tumor-suppressing multi-enterobacteria and PD-1/PD-L1 immune checkpoint inhibitor combination improves the outcome of hepatocellular carcinoma therapy.

Applications

Unspecified application

Species

Unspecified reactive species

Dongjuan Wu,Yaohua Fan,Mingsheng Zhang,Xiaoguang Wang,Xuesong He,Xinwei Guo,Yangchen Liu,Chenxi Cao,Zhaoqun Deng

View all publications

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Abcam Product Promise

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redFluor™ 710 Anti-CD45RA抗体[HI100]

redFluor™ 710 Anti-CD45RA antibody [HI100]

Flow Cytometry - redFluor™ 710 Anti-CD45RA antibody [HI100] (AB218648)

关键信息

宿主种属

克隆

克隆号

亚型

轻链类型

偶联物

激发波长/发射波长

不含载体蛋白

反应种属

应用