Anti-RECQL5抗体(ab224135)
Key features and details
- Rabbit polyclonal to RECQL5
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-RECQL5抗体 -
描述
兔多克隆抗体to RECQL5 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Human -
免疫原
Recombinant fragment corresponding to Human RECQL5 aa 550-700.
Database link: O94762 -
阳性对照
- WB: A549 cell lysate. IHC-P: Human skeletal muscle tissue. ICC/IF: Human testis, cervix and cerebral cortex tissue.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab224135于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 46,49,106,109 kDa.
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| IHC-P |
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100. |
| 说明 |
|---|
|
WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 46,49,106,109 kDa. |
|
IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
ICC/IF
Use a concentration of 0.25 - 2 µg/ml. Fixation/Permeabilization: PFA/Triton X-100. |
靶标
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功能
May have an important role in DNA metabolism. -
组织特异性
Ubiquitous. -
序列相似性
Belongs to the helicase family. RecQ subfamily.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain. -
细胞定位
Cytoplasm and Nucleus > nucleoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 9400 Human
- Omim: 603781 Human
- SwissProt: O94762 Human
- Unigene: 632229 Human
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别名
- ATP-dependent DNA helicase Q5 antibody
- DNA helicase antibody
- DNA helicase, RecQ like type 5 antibody
see all
图片
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Western blot - Anti-RECQL5 antibody (ab224135)All lanes : Anti-RECQL5 antibody (ab224135)
Lane 1 : A-549 cells transfected with control siRNA
Lane 2 : A-549 cells transfected with target specific siRNA probe 1
Lane 3 : A-549 cells transfected with target specific siRNA probe 2
Predicted band size: 46,49,106,109 kDaLoading control: Anti-GAPDH.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RECQL5 antibody (ab224135)Paraffin-embedded human cerebral cortex tissue stained for RECQL5 using ab224135 at 1/50 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RECQL5 antibody (ab224135)Paraffin-embedded human cervix tissue stained for RECQL5 using ab224135 at 1/50 dilution in immunohistochemical analysis.
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Immunocytochemistry/ Immunofluorescence - Anti-RECQL5 antibody (ab224135)PFA-fixed, Triton X-100 permeabilized A431 (human epidermoid carcinoma cell line) cells stained for RECQL5 (green) using ab224135 at 4 µg/ml in ICC/IF.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RECQL5 antibody (ab224135)Paraffin-embedded human testis tissue stained for RECQL5 using ab224135 at 1/50 dilution in immunohistochemical analysis.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab224135 被引用在 1 文献中.
- Elbakry A et al. ATRX and RECQ5 define distinct homologous recombination subpathways. Proc Natl Acad Sci U S A 118:N/A (2021). PubMed: 33431668