重组Anti-RECQ1抗体[EPR8049(2)] (ab172607)
![Western blot - Anti-RECQ1 antibody [EPR8049(2)] (ab172607)](https://www.abcam.cn/ps/products/172/ab172607/Images/ab172607-195840-anti-recq1-antibody-epr80492-western-blot.jpg "Western blot - Anti-RECQ1 antibody [EPR8049(2)] (ab172607)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8049(2)] to RECQ1
- Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-RECQ1抗体[EPR8049(2)]
参阅全部 RECQ1 一抗 -
描述
兔单克隆抗体[EPR8049(2)] to RECQ1 -
宿主
Rabbit -
经测试应用
适用于: Flow Cyt (Intra), WB, IHC-P, ICC/IFmore details
不适用于: IP -
种属反应性
与反应: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- HeLa, Raji, Ramos and 293T cell lysates; Human heart and thyroid gland carcinoma tissues; 293T and Ramos cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
EPR8049(2) -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab172607于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 72 kDa.
|
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| IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| ICC/IF |
1/250 - 1/500.
|
| 说明 |
|---|
|
Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB
1/1000 - 1/10000. Predicted molecular weight: 72 kDa. |
|
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
ICC/IF
1/250 - 1/500. |
靶标
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功能
DNA helicase that may play a role in the repair of DNA that is damaged by ultraviolet light or other mutagens. Exhibits a magnesium-dependent ATP-dependent DNA-helicase activity that unwinds single- and double-stranded DNA in a 3'-5' direction. -
组织特异性
High expression in heart, lung, skeletal muscle and kidney, low expression in brain. -
序列相似性
Belongs to the helicase family. RecQ subfamily.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 5965 Human
- Omim: 600537 Human
- SwissProt: P46063 Human
- Unigene: 235069 Human
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别名
- ATP dependent DNA helicase Q1 antibody
- ATP-dependent DNA helicase Q1 antibody
- DNA helicase antibody
see all
图片
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Western blot - Anti-RECQ1 antibody [EPR8049(2)] (ab172607)All lanes : Anti-RECQ1 antibody [EPR8049(2)] (ab172607) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : Raji cell lysate
Lane 3 : Ramos cell lysate
Lane 4 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 72 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RECQ1 antibody [EPR8049(2)] (ab172607)](https://www.abcam.cn/ps/products/172/ab172607/Images/ab172607-195841-anti-recq1-antibody-epr80492-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RECQ1 antibody [EPR8049(2)] (ab172607)Immunohistochemical analysis of paraffin-embedded Human heart tissue labeling RECQ1 with ab172607 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RECQ1 antibody [EPR8049(2)] (ab172607)](https://www.abcam.cn/ps/products/172/ab172607/Images/ab172607-195842-anti-recq1-antibody-epr80492-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RECQ1 antibody [EPR8049(2)] (ab172607)Immunohistochemical analysis of paraffin-embedded Human thyroid gland carcinoma tissue labeling RECQ1 with ab172607 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunocytochemistry/ Immunofluorescence - Anti-RECQ1 antibody [EPR8049(2)] (ab172607)](https://www.abcam.cn/ps/products/172/ab172607/Images/ab172607-195843-anti-recq1-antibody-epr80492-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-RECQ1 antibody [EPR8049(2)] (ab172607)Immunofluorescent analysis of 293T cells labeling RECQ1 with ab172607 at 1/250 dilution.
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![Flow Cytometry (Intracellular) - Anti-RECQ1 antibody [EPR8049(2)] (ab172607)](https://www.abcam.cn/ps/products/172/ab172607/Images/ab172607-195844-anti-recq1-antibody-epr80492-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-RECQ1 antibody [EPR8049(2)] (ab172607)Intracellular flow cytometric analysis of permeabilized Ramoscells labeling RECQ1 with ab172607at 1/10 dilution (red) compared to a Rabbit IgG negative control (green).
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![Anti-RECQ1 antibody [EPR8049(2)] (ab172607)](https://www.abcam.cn/ps/products/172/ab172607/Images/ab172607-6-benefits-of-recombinant-antibodies.png)
Anti-RECQ1 antibody [EPR8049(2)] (ab172607)
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (0)
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