Anti-RECQ1抗体(ab272855)
Key features and details
- Rabbit polyclonal to RECQ1
- Suitable for: WB, IHC-P, ICC/IF, IP
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-RECQ1抗体
参阅全部 RECQ1 一抗 -
描述
兔多克隆抗体to RECQ1 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IF, IPmore details -
种属反应性
与反应: Human -
免疫原
Recombinant fragment within Human RECQ1 (N terminal). The exact sequence is proprietary.
Database link: P46063 -
阳性对照
- WB: A431, HeLa and A375 whole cell lysate. IHC-P: Human breast carcinoma tissue. ICC/IF: HeLa cells. IP: HeLa whole cell lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab272855于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/500 - 1/3000.
|
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| IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
|
| ICC/IF |
1/100 - 1/1000.
|
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| IP |
1/100 - 1/500.
|
| 说明 |
|---|
|
WB
1/500 - 1/3000. |
|
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
|
ICC/IF
1/100 - 1/1000. |
|
IP
1/100 - 1/500. |
靶标
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功能
DNA helicase that may play a role in the repair of DNA that is damaged by ultraviolet light or other mutagens. Exhibits a magnesium-dependent ATP-dependent DNA-helicase activity that unwinds single- and double-stranded DNA in a 3'-5' direction. -
组织特异性
High expression in heart, lung, skeletal muscle and kidney, low expression in brain. -
序列相似性
Belongs to the helicase family. RecQ subfamily.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 5965 Human
- Omim: 600537 Human
- SwissProt: P46063 Human
- Unigene: 235069 Human
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别名
- ATP dependent DNA helicase Q1 antibody
- ATP-dependent DNA helicase Q1 antibody
- DNA helicase antibody
see all
图片
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Western blot - Anti-RECQ1 antibody (ab272855)All lanes : Anti-RECQ1 antibody (ab272855) at 1/1000 dilution
Lane 1 : A431 (human epidermoid carcinoma cell line) whole cell lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 3 : A-375 (human malignant melanoma cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.7.5% SDS-PAGE.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RECQ1 antibody (ab272855)Paraffin-embedded human breast carcinoma tissue stained for RECQ1 using ab272855 at 1/250 dilution in immunohistochemical analysis.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15 mins. -
Immunocytochemistry/ Immunofluorescence - Anti-RECQ1 antibody (ab272855)Immunofluorescence analysis of 4% paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling RECQ1 (green) using ab272855 at 1/500 dilution.
Counterstain: Phalloidin, a cytoskeleton marker (red).
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Immunoprecipitation - Anti-RECQ1 antibody (ab272855)Immunoprecipitation of RECQ1 protein from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate using ab272855 at 5 μg. ab272855 also used for WB followed by an anti-Rabbit IgG-HRP secondary.
Lane 1: Control IgG.
Lane 2: ab272855 IP in HeLa whole cell lysate.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab272855 尚未被引用在任何文献中。