Anti-RecA抗体(ab63797)
Key features and details
- Rabbit polyclonal to RecA
- Suitable for: WB, IP
- Reacts with: Escherichia coli
- Isotype: IgG
概述
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产品名称
Anti-RecA抗体 -
描述
兔多克隆抗体to RecA -
宿主
Rabbit -
经测试应用
适用于: WB, IPmore details -
种属反应性
与反应: Escherichia coli -
免疫原
Recombinant full length RecA (Escherichia coli) highly purified by chromatography.
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阳性对照
- Extract of E.coli AB1157.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 6
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab63797于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (4) |
1/3000. Detects a band of approximately 42 kDa (predicted molecular weight: 38 kDa).
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| IP |
1/600.
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| 说明 |
|---|
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WB
1/3000. Detects a band of approximately 42 kDa (predicted molecular weight: 38 kDa). |
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IP
1/600. |
靶标
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相关性
RecA in E.coli can catalyze the hydrolysis of ATP in the presence of single-stranded DNA, the ATP-dependent uptake of single-stranded DNA by duplex DNA, and the ATP-dependent hybridization of homologous single-stranded DNAs. It interacts with lexA causing its activation and leading to its autocatalytic cleavage. -
细胞定位
Cytoplasmic -
数据库链接
- Entrez Gene: 947170 Escherichia coli
- SwissProt: P0A7G6 Escherichia coli
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别名
- DNA strand exchange and recombination protein with protease and nuclease activity antibody
- lexB antibody
- Protein recA antibody
see all
图片
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Immunoprecipitation - Anti-RecA antibody (ab63797)
Immunoprecipitation of RecA protein from crudeextract of E. coli cells. 2 μg of ab63797 was adsorbed to proteinA magnetic beads to precipitate RecA protein from supernatant of sonic disrupted E. coli cells. The precipitate was analyzed by western blot.
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Western blot - Anti-RecA antibody (ab63797)All lanes : Anti-RecA antibody (ab63797) at 1/3000 dilution
Lane 1 : Molecular weight markers
Lane 2 : Purified RecA
Lane 3 : Extract of E.coli AB1157
Predicted band size: 38 kDa
Observed band size: 42 kDa why is the actual band size different from the predicted?
数据表及文件
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SDS download
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Datasheet download
文献 (39)
ab63797 被引用在 39 文献中.
- Nguyen TT et al. Exploring the Potential of a Genome-Reduced Escherichia coli Strain for Plasmid DNA Production. Int J Mol Sci 24:N/A (2023). PubMed: 37511505
- Mitosch K et al. A pathogen-specific isotope tracing approach reveals metabolic activities and fluxes of intracellular Salmonella. PLoS Biol 21:e3002198 (2023). PubMed: 37594988
- Xu J et al. Identification and structure of an extracellular contractile injection system from the marine bacterium Algoriphagus machipongonensis. Nat Microbiol 7:397-410 (2022). PubMed: 35165385
- Laureti L et al. Single strand gap repair: The presynaptic phase plays a pivotal role in modulating lesion tolerance pathways. PLoS Genet 18:e1010238 (2022). PubMed: 35653392
- Zlatkov N et al. Metabolic and Morphotypic Trade-Offs within the Eco-Evolutionary Dynamics of Escherichia coli. Microbiol Spectr 10:e0067822 (2022). PubMed: 36169422