重组Anti-RCN1/RCN抗体[EPR17163] - C-terminal (ab198996)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR17163] to RCN1/RCN - C-terminal
- Suitable for: Flow Cyt (Intra), IHC-P, WB, ICC/IF, IP
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-RCN1/RCN抗体[EPR17163] - C-terminal
参阅全部 RCN1/RCN 一抗 -
描述
兔单克隆抗体[EPR17163] to RCN1/RCN - C-terminal -
宿主
Rabbit -
经测试应用
适用于: Flow Cyt (Intra), IHC-P, WB, ICC/IF, IPmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: 293 cell lysate and HeLa cell lysate. IHC: Human cerebral cortex tissue. ICC/IF: HeLa cells. IP and Flow Cyt (intra): HeLa cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR17163 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab198996于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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Flow Cyt (Intra) |
1/70.
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IHC-P |
1/800. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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WB |
1/1000. Detects a band of approximately 44, 46 kDa (predicted molecular weight: 39 kDa).
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ICC/IF |
1/250.
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IP |
1/100.
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说明 |
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Flow Cyt (Intra)
1/70. |
IHC-P
1/800. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
WB
1/1000. Detects a band of approximately 44, 46 kDa (predicted molecular weight: 39 kDa). |
ICC/IF
1/250. |
IP
1/100. |
靶标
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功能
May regulate calcium-dependent activities in the endoplasmic reticulum lumen or post-ER compartment. -
序列相似性
Belongs to the CREC family.
Contains 6 EF-hand domains. -
细胞定位
Endoplasmic reticulum lumen. - Information by UniProt
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数据库链接
- Entrez Gene: 5954 Human
- Entrez Gene: 19672 Mouse
- Entrez Gene: 362182 Rat
- Omim: 602735 Human
- SwissProt: Q15293 Human
- SwissProt: Q05186 Mouse
- Unigene: 97887 Human
- Unigene: 397500 Mouse
see all -
别名
- Epididymis secretory protein Li 84 antibody
- FLJ37041 antibody
- HEL S 84 antibody
see all
图片
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All lanes : Anti-RCN1/RCN antibody [EPR17163] - C-terminal (ab198996) at 1/10000 dilution
Lane 1 : 293 (Human epithelial cells from embryonic kidney) cell lysate
Lane 2 : HeLa (Human epithelial cells from cervix adenocarcinoma) cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 39 kDa
Observed band size: 44, 46 kDa why is the actual band size different from the predicted?
Exposure time: 1 minuteBlocking and diluting buffer was 5% NFDM/TBST.
The expression profile observed is consistent with what has been described in the literature PMID: 8416973.
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Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling RCN1/RCN with ab198996 at 1/500. Cells were fixed with 100% methanol. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody.
Control: PBS only.
Nuclear counter stain: DAPI. -
Immunohistochemical analysis of paraffin-embedded Human cerebral cortex tissue labeling RCN1/RCN with ab198996 at 1/1600, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500. Cytoplasm staining on Human cerebral cortex tissue is observed. Subcellular location Endoplasmic reticulum lumen [UniProt]. Counter stained with Hematoxylin.
Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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All lanes : Anti-RCN1/RCN antibody [EPR17163] - C-terminal (ab198996) at 1/1000 dilution
Lane 1 : Raw264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) cell lysate
Lane 2 : Rat heart tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 39 kDa
Observed band size: 44, 46 kDa why is the actual band size different from the predicted?
Exposure time: 1 minuteBlocking and diluting buffer was 5% NFDM/TBST.
The expression profile observed is consistent with what has been described in the literature PMID: 8416973. -
RCN1/RCN was immunoprecipitated from 1mg of HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell extract with ab198996 at 1/100. Western blot was performed from the immunoprecipitate using ab198996 at 1/1000. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500.
Lane 1: HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell extract. 10ug (Input.
Lane 2: HeLa whole cell extract.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab198996 in HeLa whole cell extract.
The expression profile observed is consistent with what has been described in the literature PMID: 8416973.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
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Intracellular Flow Cytometry analysis of HeLa cells labelling RCN1/RCN (red) with purified ab198996 at dilution of 1/70. The secondary antibody used was Alexa Fluorr® 488 goat-anti-rabbit IgG (1/2000). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Isotype control antibody was Rabbit monoclonal IgG (black). The blue line shows cells without incubation with primary antibody and secondary antibody.
实验方案
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (0)
ab198996 尚未被引用在任何文献中。