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重组RabMAb

重组Anti-RCC1抗体[EPR5857] - BSA and Azide free (ab226061)

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Western blot - Anti-RCC1 antibody [EPR5857] - BSA and Azide free (ab226061)
  • Western blot - Anti-RCC1 antibody [EPR5857] - BSA and Azide free (ab226061)
  • Western blot - Anti-RCC1 antibody [EPR5857] - BSA and Azide free (ab226061)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RCC1 antibody [EPR5857] - BSA and Azide free (ab226061)
  • Flow Cytometry (Intracellular) - Anti-RCC1 antibody [EPR5857] - BSA and Azide free (ab226061)
  • Anti-RCC1 antibody [EPR5857] - BSA and Azide free (ab226061)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5857] to RCC1 - BSA and Azide free
  • Suitable for: IHC-P, WB, Flow Cyt (Intra)
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Unconjugated

概述

  • 产品名称

    Anti-RCC1抗体[EPR5857] - BSA and Azide free
    参阅全部 RCC1 一抗

  • 描述

    兔单克隆抗体[EPR5857] to RCC1 - BSA and Azide free

  • 宿主

    Rabbit

  • 特异性

    This product does not react with Mouse or Rat in IHC-P

  • 经测试应用

    适用于: IHC-P, WB, Flow Cyt (Intra)more details

  • 种属反应性

    与反应: Mouse, Rat, Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • WB: mouse kidney, mouse spleen, C6, RAW 264.7, PC-12, NIH/3T3, A431, HeLa, Jurkat and 293T cell lysates. IHC-P: Human bladder carcinoma tissue. Flow Cyt (intra): HeLa cells.

  • 常规说明

    ab226061 is the carrier-free version of ab109379.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab226061于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Heat up to 98°C, below boiling, and then let cool for 10-20 min.
WB
Use at an assay dependent concentration. Predicted molecular weight: 45 kDa.
Flow Cyt (Intra)
Use at an assay dependent concentration.
说明
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Heat up to 98°C, below boiling, and then let cool for 10-20 min.
WB
Use at an assay dependent concentration. Predicted molecular weight: 45 kDa.
Flow Cyt (Intra)
Use at an assay dependent concentration.

靶标

  • 功能

    Guanine-nucleotide releasing factor that promotes the exchange of Ran-bound GDP by GTP. Involved in the regulation of onset of chromosome condensation in the S phase. Binds both to the nucleosomes and double-stranded DNA. RCC1-Ran complex (together with other proteins) acts as a component of a signal transmission pathway that detects unreplicated DNA. Plays a key role in nucleo-cytoplasmic transport, mitosis and nuclear-envelope assembly.

  • 序列相似性

    Contains 7 RCC1 repeats.

  • 翻译后修饰

    N-terminal methylation by METTL11A/NTM1 is required for binding double-stranded DNA and stable chromatin association. Di-and trimethylation produce a permanent positive charge on the amino group, which facilitate electrostatic binding to the phosphate groups on DNA, while inhibiting histone-binding. Methylated tail helps retain RCC1 on chromosomes during nucleotide exchange on Ran.

  • 细胞定位

    Nucleus. Cytoplasm. Becomes dispersed throughout the cytoplasm during mitosis.
  • Information by UniProt

  • 数据库链接

    • 别名

      • Cell cycle regulatory protein antibody
      • CHC 1 antibody
      • CHC1 antibody
      • Chromosome condensation 1 antibody
      • Chromosome condensation protein 1 antibody
      • Guanine nucleotide releasing protein antibody
      • HERC2 antibody
      • Ran GEF antibody
      • RanGEF antibody
      • RCC 1 antibody
      • RCC1 antibody
      • RCC1 I antibody
      • RCC1_HUMAN antibody
      • Regulator of chromosome condensation 1 antibody
      • Regulator of chromosome condensation antibody
      • SHEP1 antibody
      • SNHG3 RCC1 antibody
      • SNHG3 RCC1 readthrough transcript antibody
      see all

    图片

    • Western blot - Anti-RCC1 antibody [EPR5857] - BSA and Azide free (ab226061)
      Western blot - Anti-RCC1 antibody [EPR5857] - BSA and Azide free (ab226061)
      All lanes : Anti-RCC1 antibody [EPR5857] (ab109379) at 1/1000 dilution

      Lane 1 : Mouse kidney tissue lysate
      Lane 2 : Mouse spleen tissue lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 45 kDa
      Observed band size: 44 kDa why is the actual band size different from the predicted?


      Exposure time: 180 seconds


      This data was developed using ab109379, the same antibody clone in a different buffer formulation

      Diluting and blocking buffer: 5% NFDM/TBST 5% NFDM/TBST

       

    • Western blot - Anti-RCC1 antibody [EPR5857] - BSA and Azide free (ab226061)
      Western blot - Anti-RCC1 antibody [EPR5857] - BSA and Azide free (ab226061)
      All lanes : Anti-RCC1 antibody [EPR5857] (ab109379) at 1/1000 dilution

      Lane 1 : C6 (rat glial tumor glial cell) whole cell lysate
      Lane 2 : RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate
      Lane 3 : PC-12 (rat adrenal gland pheochromocytoma cell) whole cell lysate
      Lane 4 : NIH/3T3 (mouse embryonic fibroblast) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 45 kDa
      Observed band size: 44 kDa why is the actual band size different from the predicted?


      Exposure time: 180 seconds


      This data was developed using ab109379, the same antibody clone in a different buffer formulation

      Diluting and blocking buffer: 5% NFDM/TBST 5% NFDM/TBST

       

    • Western blot - Anti-RCC1 antibody [EPR5857] - BSA and Azide free (ab226061)
      Western blot - Anti-RCC1 antibody [EPR5857] - BSA and Azide free (ab226061)
      All lanes : Anti-RCC1 antibody [EPR5857] (ab109379) at 1/1000 dilution

      Lane 1 : A431 cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : Jurkat cell lysate
      Lane 4 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 45 kDa



      This data was developed using ab109379, the same antibody clone in a different buffer formulation

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RCC1 antibody [EPR5857] - BSA and Azide free (ab226061)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RCC1 antibody [EPR5857] - BSA and Azide free (ab226061)

      ab109379, at a 1/100 dilution, staining RCC1 in paraffin-embedded Human bladder carcinoma tissue

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109379).

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Flow Cytometry (Intracellular) - Anti-RCC1 antibody [EPR5857] - BSA and Azide free (ab226061)
      Flow Cytometry (Intracellular) - Anti-RCC1 antibody [EPR5857] - BSA and Azide free (ab226061)

      Intracellular Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling RCC1 (red) with ab109379 at a 1/2000 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730). Blue (unlabeled control) - Cells without incubation with primary and secondary antibodies. 

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109379). 

       

       

    • Anti-RCC1 antibody [EPR5857] - BSA and Azide free (ab226061)
      Anti-RCC1 antibody [EPR5857] - BSA and Azide free (ab226061)

    实验方案

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    文献 (0)

    发表研究结果有使用 ab226061？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab226061 尚未被引用在任何文献中。

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