重组Anti-RCAS1抗体[EPR16568] - BSA and Azide free

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-RCAS1 antibody [EPR16568] - BSA and Azide free (AB251310)

This data was developed using ab200348, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized T-47D (Human ductal breast epithelial tumor cell line) cells, labeling RCAS1 with ab200348 at 1/2000 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Cytoplasm staining on T47D cell line was observed. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).

The negative controls are as follows :
-ve control 1 : ab200348 at 1/2000 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
-ve control 2 : ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-RCAS1 antibody [EPR16568] - BSA and Azide free (AB251310)

This data was developed using ab200348, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HEK293 (Human embryonic kidney) cells labeling RCAS1 with ab200348 at 1/2000 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Cytoplasm staining on HEK293 cell line was observed. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).

The negative controls are as follows :
-ve control 1 : ab200348 at 1/2000 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
-ve control 2 : ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.

• IP

Supplier Data

Immunoprecipitation - Anti-RCAS1 antibody [EPR16568] - BSA and Azide free (AB251310)

This data was developed using ab200348, the same antibody clone in a different buffer formulation.

RCAS1 was immunoprecipitated from 1mg of HEK293 (Human embryonic kidney) whole cell lysate with ab200348 at 1/80 dilution. Western blot was performed from the immunoprecipitate using ab200348 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

Lane 1 : HEK293 whole cell lysate 10 μg (Input). Lane 2 : ab200348 IP in HEK293 whole cell lysate. Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab200348 in HEK293 whole cell lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-RCAS1 antibody [EPR16568] (<a href='/products/primary-antibodies/rcas1-antibody-epr16568-ab200348'>ab200348</a>)

Predicted band size: 24 kDa

false

• WB

Supplier Data

Western blot - Anti-RCAS1 antibody [EPR16568] - BSA and Azide free (AB251310)

This data was developed using ab200348, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-RCAS1 antibody [EPR16568] (<a href='/products/primary-antibodies/rcas1-antibody-epr16568-ab200348'>ab200348</a>) at 1/1000 dilution

All lanes:

T-47D (Human ductal breast epithelial tumor cell line) whole cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 24 kDa

Observed band size: 32 kDa

false

Exposure time: 30s

• WB

Supplier Data

Western blot - Anti-RCAS1 antibody [EPR16568] - BSA and Azide free (AB251310)

This data was developed using ab200348, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-RCAS1 antibody [EPR16568] (<a href='/products/primary-antibodies/rcas1-antibody-epr16568-ab200348'>ab200348</a>) at 1/1000 dilution

Lane 1:

Jurkat (Human T cell leukemia cells from peripheral blood ) whole cell lysate at 10 µg

Lane 2:

293 (Human embryonic kidney) whole cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 24 kDa

Observed band size: 24 kDa,32 kDa

false

Exposure time: 3min

• WB

Supplier Data

Western blot - Anti-RCAS1 antibody [EPR16568] - BSA and Azide free (AB251310)

This data was developed using ab200348, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-RCAS1 antibody [EPR16568] (<a href='/products/primary-antibodies/rcas1-antibody-epr16568-ab200348'>ab200348</a>) at 1/5000 dilution

Lane 1:

Human fetal brain tissue lysate at 10 µg

Lane 2:

Human fetal kidney tissue lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 24 kDa

Observed band size: 32 kDa

false

Exposure time: 3min