Anti-RC3H1抗体(ab70196)
Key features and details
- Rabbit polyclonal to RC3H1
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-RC3H1抗体
参阅全部 RC3H1 一抗 -
描述
兔多克隆抗体to RC3H1 -
宿主
Rabbit -
经测试应用
适用于: WB, IPmore details -
种属反应性
与反应: Human
预测可用于: Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Bat
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免疫原
Synthetic peptide corresponding to a region between C terminal residues 1075 and 1133 of human RC3H1 (NP_742068.1)
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阳性对照
- HeLa and 293T whole cell lysates.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
ab70196 was affinity purified using an epitope specific to RC3H1 immobilized on solid support. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab70196于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | ||
| IP |
| 说明 |
|---|
WB: 1/1000 - 1/10000. Detects a band of approximately 150 kDa (predicted molecular weight: 126 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
靶标
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功能
Represses ICOS function, an essential costimulatory receptor for follicular T-cells. May act as an ubiquitin ligase that regulates ubiquitin-dependent control of AU-rich mRNAs by AUF1/HNRPD and HUR/ELAVL1. -
组织特异性
Widely expressed. Expressed at higher level in cerebellum, spleen, ovary and liver. -
序列相似性
Contains 1 C3H1-type zinc finger.
Contains 1 RING-type zinc finger. -
细胞定位
Cytoplasm. Localizes to cytosolic RNA granules, which are sites for regulating mRNA translation and decay, suggesting that it may bind RNA. - Information by UniProt
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数据库链接
- Entrez Gene: 149041 Human
- Omim: 609424 Human
- SwissProt: Q5TC82 Human
- Unigene: 30258 Human
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别名
- KIAA2025 antibody
- Rc3h1 antibody
- RC3H1_HUMAN antibody
see all
图片
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Western blot - Anti-RC3H1 antibody (ab70196)All lanes : Anti-RC3H1 antibody (ab70196) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Predicted band size: 126 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted?
Exposure time: 30 seconds -
Immunoprecipitation - Anti-RC3H1 antibody (ab70196)Western Blot showing Lane 2: ab70196 at 0.04µg/ml staining RC3H1 in HeLa whole cell lysates immunoprecipitated using ab70196 at 3µg/mg lysate (1 mg/IP; 1/4 of IP loaded/lane). Lane 1: an irrelevant antibody was used for immunoprecipitation. Detection: Chemiluminescence with an exposure time of 30 seconds.
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab70196 被引用在 2 文献中.
- Zhang M et al. Exosomal Mir-3613-3p derived from oxygen-glucose deprivation-treated brain microvascular endothelial cell promotes microglial M1 polarization. Cell Mol Biol Lett 28:18 (2023). PubMed: 36870962
- Hou Y et al. YTHDC1-mediated augmentation of miR-30d in repressing pancreatic tumorigenesis via attenuation of RUNX1-induced transcriptional activation of Warburg effect. Cell Death Differ 28:3105-3124 (2021). PubMed: 34021267