JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB109193

重组Anti-RBP4抗体[EP3657]

Anti-RBP4 antibody [EP3657]

Be the first to review this product! Submit a review

|

(18 Publications)

Rabbit Recombinant Monoclonal RBP4 antibody. Suitable for WB, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 18 publications.

查看别名

PRO2222, RBP4, Retinol-binding protein 4, Plasma retinol-binding protein, PRBP, RBP

6 Images
Immunocytochemistry/ Immunofluorescence - Anti-RBP4 antibody [EP3657] (AB109193)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-RBP4 antibody [EP3657] (AB109193)

Immunofluorescent analysis of HeLa cells labelling RBP4 with un-purified ab109193 at a dilution of 1/100.

Immunocytochemistry/ Immunofluorescence - Anti-RBP4 antibody [EP3657] (AB109193)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-RBP4 antibody [EP3657] (AB109193)

Immunocytochemistry/immunofluorescence staining of 100% methanol fixed; 0.1% triton X 100 permeabilized HeLa (human cervix adenocarcinoma) cells with purified ab109193 at dilution of 1/100. The secondary antibody used was Alexa Fluor® 488; goat anti-rabbit IgG (ab150077) at a dilution of 1/1000. Nucleus was counter-stained with DAPI (blue). ab7291, a mouse anti-tubulin antibody (1/1000) was used to stain tubulin along with ab150120 (AlexaFluor®594 goat anti-mouse secondary, 1/1000) shown in the top right hand panel. The negative controls are shown in the bottom middle and right hand panels- for negative control 1 rabbit primary antibody and anti-mouse secondary antibody (ab150120) was used. For negative control 2 mouse primary antibody (ab7291) and anti-rabbit secondary antibody (ab150077) was used.

Western blot - Anti-RBP4 antibody [EP3657] (AB109193)
  • WB

Supplier Data

Western blot - Anti-RBP4 antibody [EP3657] (AB109193)

Blocking and diluting buffer 5% NFDM/TBST

All lanes:

Western blot - Anti-RBP4 antibody [EP3657] (ab109193) at 1/5000 dilution

Lane 1:

HepG2 (human hepatocellular carcinoma) whole cell lysate at 20 µg

Lane 2:

Human fetal kidney at 20 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 23 kDa

Observed band size: 23 kDa

false

Western blot - Anti-RBP4 antibody [EP3657] (AB109193)
  • WB

Unknown

Western blot - Anti-RBP4 antibody [EP3657] (AB109193)

All lanes:

Western blot - Anti-RBP4 antibody [EP3657] (ab109193) at 1/1000 dilution

Lane 1:

Human plasma lysate at 10 µg

Lane 2:

HepG2 cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 23 kDa

Observed band size: 23 kDa

false

Western blot - Anti-RBP4 antibody [EP3657] (AB109193)
  • WB

Supplier Data

Western blot - Anti-RBP4 antibody [EP3657] (AB109193)

Blocking and diluting buffer 5% NFDM/TBST

All lanes:

Western blot - Anti-RBP4 antibody [EP3657] (ab109193) at 1/5000 dilution

All lanes:

Mouse serum at 20 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 23 kDa

Observed band size: 23 kDa

false

Western blot - Anti-RBP4 antibody [EP3657] (AB109193)
  • WB

Unknown

Western blot - Anti-RBP4 antibody [EP3657] (AB109193)

Blocking and diluting buffer 5% NFDM/TBST

All lanes:

Western blot - Anti-RBP4 antibody [EP3657] (ab109193) at 1/5000 dilution

All lanes:

Rat serum at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 23 kDa

Observed band size: 23 kDa

false

不同偶联物与剂型 (1)

  • Carrier free

    Anti-RBP4 antibody [EP3657] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EP3657

亚型

IgG

不含载体蛋白

No

反应种属

Mouse, Rat, Human

应用

WB, ICC/IF

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
style
left-column

产品详情

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

style
left-column

性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A
存储溶液
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.18% BSA
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Stable for 12 months at -20°C
style
left-column

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

RBP4 or retinol-binding protein 4 is a carrier protein that is essential for the transport of retinol or vitamin A in the bloodstream. Also known by the names RBP or plasma retinol-binding protein this protein has a molecular weight of approximately 21 kDa. RBP4 is mainly expressed in the liver and secreted into the blood where it binds to retinol and protects it from oxidation. The structure of RBP4 allows it to fit into binding pockets facilitating the transport of retinol to peripheral tissues.
Biological function summary

RBP4 plays a role as a transporter of vitamin A from the liver to tissues where it is needed. It forms complexes with transthyretin in the blood which prevents kidney filtration and subsequent loss of retinol. This function ensures the proper delivery and availability of retinol essential for vision cellular differentiation and immune system function. The stability of the retinol-RBP4 complex is important to maintaining sufficient vitamin A levels in the body.

Pathways

RBP4 is significant in the vitamin A metabolism pathway and affects the retinoid signaling pathways. These pathways are critical for processes such as vision and skin health. RBP4 interacts with proteins like lecithin retinol acyltransferase (LRAT) in these pathways which are responsible for esterifying retinol into retinyl esters a storage form of vitamin A. These interactions ensure the efficient transport and storage of vitamin A and that it is accessible when needed by different tissues.

RBP4 is linked to the development of insulin resistance and type 2 diabetes. Elevated RBP4 levels correlate with these conditions and highlight its role in metabolic processes. Studies also show a connection between altered RBP4 levels and obesity with insulin resistance often occurring in conjunction with obesity. The protein is implicated in these metabolic disorders through its interactions with insulin-regulating proteins which influence glucose homeostasis.
style
left-column
style
left-column

产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

靶点信息

Retinol-binding protein that mediates retinol transport in blood plasma (PubMed : 5541771). Delivers retinol from the liver stores to the peripheral tissues (Probable). Transfers the bound all-trans retinol to STRA6, that then facilitates retinol transport across the cell membrane (PubMed : 22665496).
See full target information RBP4
style
left-column

文献 (18)

Recent publications for all applications. Explore the full list and refine your search

Chinese medicine 20:77 PubMed40442809

2025

The effect and mechanism of Germacrone in ameliorating alcoholic fatty liver by inhibiting Nrf2/Rbp4.

Applications

Unspecified application

Species

Unspecified reactive species

Ru Xiao,Jiamin Fang,Qinpo Huang,Guolin He,Xia Ou,Yang De,Shuhua Gui,Yun Zhang,Maoci Wang,Yiyuan Zhong,Dawa Zeren,Yongling Long,Changhui Liu,Tianqin Xiong

Genes 16: PubMed40004479

2025

Retinol-Binding Protein 4 as a Biomarker in Cancer: Insights from a Pan-Cancer Analysis of Expression, Immune Infiltration, and Methylation.

Applications

Unspecified application

Species

Unspecified reactive species

Jia Zhao,Yaxin Liu,Lingqin Zhou,Yi Liu

Journal of cachexia, sarcopenia and muscle 15:1601-1615 PubMed39031684

2024

RBP4 promotes denervation-induced muscle atrophy through STRA6-dependent pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Kang-Zhen Zhang,Jia-Wen Li,Jin-Shui Xu,Zheng-Kai Shen,Yu-Shuang Lin,Can Zhao,Xiang Lu,Yun-Feng Rui,Wei Gao

Central-European journal of immunology 49:366-382 PubMed39944260

2024

LncRNA RMRP promotes chondrocyte injury by regulating the FOXC1/RBP4 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Jingyi Li,Gang Zhou,Te Chen,Qiao Lin,Qiupeng Yang

Microscopy research and technique 86:223-231 PubMed36354743

2022

Elevated retinal retinol-binding protein 4 levels in diabetic mice can induce retinal neurodegeneration through microglia.

Applications

Unspecified application

Species

Unspecified reactive species

Zhiwei Xu,Shi Bai,Haijian Wu,Marong Fang

Molecular reproduction and development 89:423-430 PubMed35818817

2022

miR-24-3p regulation of retinol binding protein 4 in trophoblast biofunction and preeclampsia.

Applications

Unspecified application

Species

Unspecified reactive species

Zhan Li,Xiaoli Ru,Shuzhen Wang,Guangming Cao

Bone research 10:38 PubMed35477573

2022

A high-resolution route map reveals distinct stages of chondrocyte dedifferentiation for cartilage regeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Yishan Chen,Yeke Yu,Ya Wen,Juan Chen,Junxin Lin,Zixuan Sheng,Wenyan Zhou,Heng Sun,Chengrui An,Jiansong Chen,Weiliang Wu,Chong Teng,Wei Wei,Hongwei Ouyang

Journal of the American Heart Association 10:e022011 PubMed34726071

2021

Retinol-Binding Protein 4 Promotes Cardiac Injury After Myocardial Infarction Via Inducing Cardiomyocyte Pyroptosis Through an Interaction With NLRP3.

Applications

Unspecified application

Species

Unspecified reactive species

Kang-Zhen Zhang,Xi-Yu Shen,Man Wang,Li Wang,Hui-Xian Sun,Xiu-Zhen Li,Jing-Jing Huang,Xiao-Qing Li,Cheng Wu,Can Zhao,Jia-Li Liu,Xiang Lu,Wei Gao

Nature communications 12:2783 PubMed33986294

2021

Alteration of circadian machinery in monocytes underlies chronic kidney disease-associated cardiac inflammation and fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Yuya Yoshida,Naoya Matsunaga,Takaharu Nakao,Kengo Hamamura,Hideaki Kondo,Tomomi Ide,Hiroyuki Tsutsui,Akito Tsuruta,Masayuki Kurogi,Michio Nakaya,Hitoshi Kurose,Satoru Koyanagi,Shigehiro Ohdo

American journal of physiology. Endocrinology and metabolism 320:E306-E315 PubMed33284085

2020

Retinol-binding protein 4 mRNA translation in hepatocytes is enhanced by activation of mTORC1.

Applications

Unspecified application

Species

Unspecified reactive species

Jaclyn E Welles,Allyson L Toro,Siddharth Sunilkumar,Shaunaci A Stevens,Carson J Purnell,Scot R Kimball,Michael D Dennis
View all publications
style
left-column
style
left-column
style
right-column

替代产品

Primary Antibodies

AB154914

Anti-RBP4 antibody

Immunocytochemistry/ Immunofluorescence - Anti-RBP4 antibody (AB154914)

  • 0
  • 0
Primary Antibodies

AB233138

Anti-RBP4 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBP4 antibody (AB233138)

  • 0
  • 0
Primary Antibodies

AB133530

Anti-RBP4 antibody [EPR5878]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBP4 antibody [EPR5878] (AB133530)

  • 0
  • 0
Primary Antibodies

AB188230

Anti-RBP4 antibody [EPR18020-115]

BOND RX™ Validated|RabMAb|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-RBP4 antibody [EPR18020-115] (AB188230)

  • 0
  • 0
Primary Antibodies

AB133559

Anti-RBP4 antibody [EPR5879]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-RBP4 antibody [EPR5879] (AB133559)

  • 0
  • 0
Primary Antibodies

AB227487

Anti-RBP4 antibody [EPR18020-115] - BSA and Azide free

BOND RX™ Validated|RabMAb|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-RBP4 antibody [EPR18020-115] - BSA and Azide free (AB227487)

  • 0
  • 0

搭配使用产品

Assay Kits

AB196264

Human Retinol Binding Protein 4 ELISA Kit

Recombinant|SimpleStep

ELISA - Human Retinol Binding Protein 4 ELISA Kit (AB196264)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB178846

Anti-Iba1 antibody [EPR16588] - Microglia marker

20ul selling size|BOND RX™ Validated|RabMAb|Recombinant|Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody [EPR16588] - Microglia marker (AB178846)

  • 5
  • 50
Primary Antibodies

AB205198

Anti-AACT antibody [EPR17088-68]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-AACT antibody [EPR17088-68] (AB205198)

  • 0
  • 0
Primary Antibodies

AB125001

Anti-Retinoid X Receptor alpha/RXRA antibody [EPR7106]

20ul selling size|RabMAb|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-Retinoid X Receptor alpha/RXRA antibody [EPR7106] (AB125001)

  • 5
  • 5

Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。
详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com