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AB180494

重组Anti-RBP2抗体[EPR12334]

Anti-RBP2 antibody [EPR12334]

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(1 Publication)

Rabbit Recombinant Monoclonal RET2 antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Human samples. Cited in 1 publication.

查看别名

CRBP2, RBP2, Retinol-binding protein 2, Cellular retinol-binding protein II, CRBP-II

4 Images
Western blot - Anti-RBP2 antibody [EPR12334] (AB180494)
  • WB

Supplier Data

Western blot - Anti-RBP2 antibody [EPR12334] (AB180494)

Blocking buffer and concentration : 5% NFDM/TBST

All lanes:

Western blot - Anti-RBP2 antibody [EPR12334] (ab180494) at 1/5000 dilution

All lanes:

Human small intestine tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab136636'>ab136636</a>) at 1/500 dilution

Predicted band size: 16 kDa

false

Western blot - Anti-RBP2 antibody [EPR12334] (AB180494)
  • WB

Supplier Data

Western blot - Anti-RBP2 antibody [EPR12334] (AB180494)

Blocking buffer and concentration : 5% NFDM/TBST

All lanes:

Western blot - Anti-RBP2 antibody [EPR12334] (ab180494) at 1/5000 dilution

All lanes:

Caco-2 cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab136636'>ab136636</a>) at 1/1000 dilution

Predicted band size: 16 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBP2 antibody [EPR12334] (AB180494)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBP2 antibody [EPR12334] (AB180494)

Immunohistochemical analysis of paraffin-embedded Human small intestine tissue labeling RBP2 with ab180494 at a 1/500 dilution. Prediluted HRP Polymer for Rabbit IgG was used as a secondary antibody. Counter stain : Hematoxylin

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-RBP2 antibody [EPR12334] (AB180494)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-RBP2 antibody [EPR12334] (AB180494)

Immunofluorescent analysis of 4% paraformaldehyde fixed SW480 cell line staining RBP2 using ab180494 at a 1/100 dilution. Goat anti rabbit IgG (Dylight 488) was used as a secondary antibody at a dilution of 1/250. Counter stain : Dapi.

不同偶联物与剂型 (1)

  • Carrier free

    Anti-RBP2 antibody [EPR12334] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR12334

亚型

IgG

不含载体蛋白

No

反应种属

Human

应用

WB, ICC/IF, IHC-P

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/250 - 1/500", "IHCP-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Chimpanzee": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Gorilla": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Orangutan": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rhesus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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AB108899

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为什么推荐这个？

我们推荐这个产品，因为它经常被用于相同的实验或相关的研究。

我们建议您务必查阅产品说明书，以确认其是否符合您的实验需求；若有疑问，也可联系我们的技术团队获取协助。

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产品详情

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A
存储溶液
Preservative: 0.01% Sodium azide Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

RBP2 also known as KDM5A or Lysine Demethylase 5A functions as a histone demethylase enzyme specific for H3K4me3 and H3K4me2. With a mass of about 175 kDa it influences chromatin structure by removing methyl groups from histones which affects gene transcription. This protein expresses in various tissues especially in those with rapid cell division such as embryonic stem cells and certain cancer cell lines.
Biological function summary

RBP2 contributes to the regulation of gene expression by modifying chromatin accessibility. It forms part of transcriptional complexes that involve other chromatin-remodeling proteins. Through its demethylase activity RBP2 regulates the transcriptional response to different cellular signals and contributes to processes like cell cycle progression and differentiation.

Pathways

RBP2 plays a significant role in chromatin organization and transcriptional regulation pathways. RBP2 interacts closely with other chromatin-modifying proteins such as the Polycomb group of proteins impacting the Rb-E2F pathway involved in cell cycle control. This interaction influences whether cells proceed to division or undergo differentiation making it essential for maintaining proper cell growth and development.

RBP2 has connections to cancer and developmental disorders. Its abnormal expression often associates with various cancers including leukemia and breast cancer contributing to uncontrolled cell proliferation and tumor growth. In these contexts RBP2 may interact with proteins like pRB and E2F which are important in cell cycle regulation highlighting its importance in cancer biology. Additionally mutations in RBP2 can result in developmental abnormalities further highlighting its critical role in normal cellular processes.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Intracellular transport of retinol.
See full target information RBP2
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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Cell stem cell 29:101-115.e10 PubMed34582804

2021

SATB2 preserves colon stem cell identity and mediates ileum-colon conversion via enhancer remodeling.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Gu,Hua Wang,Xiaofeng Huang,Judith Kraiczy,Pratik N P Singh,Charles Ng,Sezin Dagdeviren,Sean Houghton,Oscar Pellon-Cardenas,Ying Lan,Yaohui Nie,Jiaoyue Zhang,Kushal K Banerjee,Emily J Onufer,Brad W Warner,Jason Spence,Ellen Scherl,Shahin Rafii,Richard T Lee,Michael P Verzi,David Redmond,Randy Longman,Kristian Helin,Ramesh A Shivdasani,Qiao Zhou
View all publications
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Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。
详情请参阅我们的条款与条件。
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