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重组RabMAb

重组Anti-RBMX2抗体[EPR14394] (ab180940)

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Immunocytochemistry/ Immunofluorescence - Anti-RBMX2 antibody [EPR14394] (ab180940)
  • Western blot - Anti-RBMX2 antibody [EPR14394] (ab180940)
  • Western blot - Anti-RBMX2 antibody [EPR14394] (ab180940)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBMX2 antibody [EPR14394] (ab180940)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBMX2 antibody [EPR14394] (ab180940)
  • Immunocytochemistry/ Immunofluorescence - Anti-RBMX2 antibody [EPR14394] (ab180940)
  • Anti-RBMX2 antibody [EPR14394] (ab180940)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14394] to RBMX2
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Mouse, Human

Conjugates logo Related conjugates and formulations

Carrier Free

概述

  • 产品名称

    Anti-RBMX2抗体[EPR14394]
    参阅全部 RBMX2 一抗

  • 描述

    兔单克隆抗体[EPR14394] to RBMX2

  • 宿主

    Rabbit

  • 经测试应用

    适用于: WB, IHC-P, ICC/IFmore details

  • 种属反应性

    与反应: Mouse, Human
    预测可用于: Rat

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • HepG2, Hela and HT-1376; Human ovarian carcinoma and mouse testis tissues; HepG2 and Hela cells.

  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • 存储溶液

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • 纯度

    Protein A purified

  • 克隆

    单克隆

  • 克隆编号

    EPR14394

  • 同种型

    IgG

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab180940于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
1/1000 - 1/10000. Detects a band of approximately 37 kDa (predicted molecular weight: 37 kDa).
IHC-P
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF
1/100.
说明
WB
1/1000 - 1/10000. Detects a band of approximately 37 kDa (predicted molecular weight: 37 kDa).
IHC-P
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF
1/100.

靶标

图片

  • Immunocytochemistry/ Immunofluorescence - Anti-RBMX2 antibody [EPR14394] (ab180940)
    Immunocytochemistry/ Immunofluorescence - Anti-RBMX2 antibody [EPR14394] (ab180940)

    Immunofluorescent analysis of 4% paraformaldehyde fixed HepG2 cell line staining RBMX2 using ab180940 at a 1/100 dilution. Goat anti rabbit IgG (Dylight 488), at a dilution of 1/200, was used as a secondary antibody. Counter stain: Dapi (blue).

  • Western blot - Anti-RBMX2 antibody [EPR14394] (ab180940)
    Western blot - Anti-RBMX2 antibody [EPR14394] (ab180940)
    All lanes : Anti-RBMX2 antibody [EPR14394] (ab180940) at 1/10000 dilution

    Lane 1 : HepG2 cell lysate
    Lane 2 : HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 37 kDa



    Blocking buffer and concentration: 5% NFDM/TBST

  • Western blot - Anti-RBMX2 antibody [EPR14394] (ab180940)
    Western blot - Anti-RBMX2 antibody [EPR14394] (ab180940)
    Anti-RBMX2 antibody [EPR14394] (ab180940) at 1/2000 dilution + HT-1376 at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 37 kDa



    Blocking buffer and concentration: 5% NFDM/TBST

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBMX2 antibody [EPR14394] (ab180940)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBMX2 antibody [EPR14394] (ab180940)

    Immunohistochemical analysis of paraffin-embedded Human ovarian carcinoma tissue labeling RBMX2 with ab180940 at a 1/100 dilution. Prediluted ImmunoHistoprobe (ready to use) HRP Polymer for Rabbit IgG was used as a secondary antibody. Counter stain: Hematoxylin

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBMX2 antibody [EPR14394] (ab180940)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBMX2 antibody [EPR14394] (ab180940)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue labeling RBMX2 with ab180940 at a 1/100 dilution. Prediluted ImmunoHistoprobe (ready to use) HRP Polymer for Rabbit IgG was used as a secondary antibody. Counter stain: Hematoxylin

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-RBMX2 antibody [EPR14394] (ab180940)
    Immunocytochemistry/ Immunofluorescence - Anti-RBMX2 antibody [EPR14394] (ab180940)

    Immunofluorescent analysis of -20℃ acetone-fixed HeLa cell line staining RBMX2 using ab180940 at a 1/100 dilution. Goat anti rabbit IgG (Dylight 488), at a dilution of 1/200, was used as a secondary antibody. Counter stain: Dapi (blue).

  • Anti-RBMX2 antibody [EPR14394] (ab180940)
    Anti-RBMX2 antibody [EPR14394] (ab180940)

文献 (0)

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