Anti-RBM35A + RBM35B抗体[23A7.C9] (ab106555)
![Western blot - Anti-RBM35A + RBM35B antibody [23A7.C9] (ab106555)](https://www.abcam.cn/ps/datasheet/images/106/ab106555/RBM35A-RBM35B-Primary-antibodies-ab106555-1.jpg "Western blot - Anti-RBM35A + RBM35B antibody [23A7.C9] (ab106555)")
Key features and details
- Mouse monoclonal [23A7.C9] to RBM35A + RBM35B
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG1
概述
-
产品名称
Anti-RBM35A + RBM35B抗体[23A7.C9] -
描述
小鼠单克隆抗体[23A7.C9] to RBM35A + RBM35B -
宿主
Mouse -
特异性
This antibody reacts with both mouse Esrp-1 and Esrp-2 proteins. A BLAST analysis of the immunizing protein sequence shows 100% homology with Esrp-1 from mouse and a 91% sequence homology with Esrp-1 from human, pig, rat, opossum, horse, cattle, panda, dog, and chimpanzee. The binding epitope of this monoclonal antibody has not been mapped.
-
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human
预测可用于: Rat, Horse, Cow, Dog, Pig, Chimpanzee, Opossum
-
免疫原
Recombinant full length Mouse RBM35A with a proprietary tag (NP_918944.2).
-
阳性对照
- RBM35A or RBM35B transfected 293T cell extracts
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.424% Potassium phosphate solution, 0.88% Sodium chloride -
Concentration information loading... -
纯度
Protein A purified -
纯化说明
This antibody was purified from tissue culture supernatant by Protein-A chromatography followed by extensive dialysis against the buffer. -
克隆
单克隆 -
克隆编号
23A7.C9 -
同种型
IgG1 -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab106555于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000. Predicted molecular weight: 76, 77 kDa.
|
| 说明 |
|---|
|
WB
1/1000. Predicted molecular weight: 76, 77 kDa. |
靶标
-
功能
mRNA splicing factor that regulates the formation of epithelial cell-specific isoforms. Specifically regulates the expression of FGFR2-IIIb, an epithelial cell-specific isoform of FGFR2. Also regulates the splicing of CD44, CTNND1, ENAH, 3 transcripts that undergo changes in splicing during the epithelial-to-mesenchymal transition (EMT). Acts by directly binding specific sequences in mRNAs. Binds the GU-rich sequence motifs in the ISE/ISS-3, a cis-element regulatory region present in the mRNA of FGFR2. -
组织特异性
Epithelial cell-specific. Epithelial-specific expression in diverse tissues and organs with particularly notable levels of expression in skin and gastrointestinal epithelia. -
序列相似性
Belongs to the ESRP family.
Contains 3 RRM (RNA recognition motif) domains. -
细胞定位
Nucleus. - Information by UniProt
-
数据库链接
- Entrez Gene: 454187 Chimpanzee
- Entrez Gene: 464289 Chimpanzee
- Entrez Gene: 510878 Cow
- Entrez Gene: 538640 Cow
- Entrez Gene: 487054 Dog
- Entrez Gene: 489745 Dog
- Entrez Gene: 100055214 Horse
- Entrez Gene: 54845 Human
see all -
别名
- Epithelial splicing regulatory protein 1 antibody
- Epithelial splicing regulatory protein 2 antibody
- Esrp1 antibody
see all
图片
-
Western blot - Anti-RBM35A + RBM35B antibody [23A7.C9] (ab106555)All lanes : Anti-RBM35A + RBM35B antibody [23A7.C9] (ab106555) at 1/1000 dilution
Lane 1 : GFP transfected 293T cell extract
Lane 2 : RBM35A transfected 293T cell extract
Lane 3 : RBM35B transfected 293T cell extract
Lysates/proteins at 5 µg per lane.
Secondary
All lanes : anti-mouse ECL antibody at 1/10000 dilution
Developed using the ECL technique.
Predicted band size: 76, 77 kDa
数据表及文件
-
SDS download
-
Datasheet download
文献 (4)
ab106555 被引用在 4 文献中.
- Chen KJ et al. Bleomycin-enhanced alternative splicing of fibroblast growth factor receptor 2 induces epithelial to mesenchymal transition in lung fibrosis. Biosci Rep 38:N/A (2018). PubMed: 30049844
- Jing J et al. Dynamic changes of urinary proteins in a rat model of acute hypercoagulable state induced by tranexamic acid. J Cell Physiol N/A:N/A (2018). PubMed: 30536986
- Chen L et al. Up-regulated miR-133a orchestrates epithelial-mesenchymal transition of airway epithelial cells. Sci Rep 8:15543 (2018). PubMed: 30341388
- Warzecha CC et al. An ESRP-regulated splicing programme is abrogated during the epithelial-mesenchymal transition. EMBO J 29:3286-300 (2010). PubMed: 20711167