Anti-RBM19抗体(ab122515)
Key features and details
- Rabbit polyclonal to RBM19
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
概述
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产品名称
Anti-RBM19抗体 -
描述
兔多克隆抗体to RBM19 -
宿主
Rabbit -
经测试应用
适用于: IHC-P, ICC/IF, WBmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Recombinant fragment corresponding to amino acids 377-486 of Human RBM19 (Q9Y4C8)
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阳性对照
- Human stomach tissue, A431 cells; RT4 and U251 MG cell lysates; Human tonsil lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20ºC. -
存储溶液
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab122515于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
1/1000. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. Use buffer at pH 6 for antigen retrieval.
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| ICC/IF |
Use a concentration of 1 - 4 µg/ml. The following steps are recommended prior to antibody incubation: cells fixation in ice cold 4% paraformaldehyde pH 7.2-7.3, followed by permeabilization with 0.1% Triton X-100 in PBS for 3x5 minutes.
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| WB |
Use a concentration of 0.04 - 0.4 µg/ml.
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| 说明 |
|---|
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IHC-P
1/1000. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. Use buffer at pH 6 for antigen retrieval. |
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ICC/IF
Use a concentration of 1 - 4 µg/ml. The following steps are recommended prior to antibody incubation: cells fixation in ice cold 4% paraformaldehyde pH 7.2-7.3, followed by permeabilization with 0.1% Triton X-100 in PBS for 3x5 minutes. |
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WB
Use a concentration of 0.04 - 0.4 µg/ml. |
靶标
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功能
Plays a role in embryo pre-implantation development. -
组织特异性
Expressed in the crypts of Lieberkuhn of the intestine and in intestinal neoplasia (at protein level). -
序列相似性
Belongs to the RRM MRD1 family.
Contains 6 RRM (RNA recognition motif) domains. -
细胞定位
Nucleus > nucleolus. Nucleus > nucleoplasm. Cytoplasm. Chromosome. In discrete foci distributed throughout the cytoplasm and nucleoplasm during the 4 to 8 cell stages and the morula stage, but not in the periphery of the nucleolar precursor body (NPB). During blastocyst development, becomes increasingly localized to the nucleolus and less to the cytoplasm. At the late blastocyst stage, localized predominantly in the nucleolus. Localized in the nucleolus during interphase and to the perichromosomal sheath during mitosis. Does not colocalize in the cytoplasm with GW182 in P-bodies. May translocate to the nucleolus upon early embryonic development (By similarity). Colocalizes with NPM1 during interphase. By late prophase, metaphase, anaphase and telophase, associates with the chromosome periphery. By telophase localizes to NPB. - Information by UniProt
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数据库链接
- Entrez Gene: 9904 Human
- Entrez Gene: 74111 Mouse
- Entrez Gene: 304512 Rat
- SwissProt: Q9Y4C8 Human
- SwissProt: Q8R3C6 Mouse
- Unigene: 7482 Human
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别名
- 1200009A02Rik antibody
- AV302714 antibody
- NPO antibody
see all
图片
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Western blot - Anti-RBM19 antibody (ab122515)All lanes : Anti-RBM19 antibody (ab122515) at 1/100 dilution
Lane 1 : NIH-3T3 cell lysate (Mouse embryonic cells)
Lane 2 : NBT-II cell lysate (Rat Wistar bladder tumour cells) -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBM19 antibody (ab122515)ab122515 at 1/1000 dilution staining RBM19 in Paraffin-embedded Human Stomach tissue by Immunohistochemistry.
Note: moderate cytoplasmic and nuclear positivity in glandular cells. -
Immunocytochemistry/ Immunofluorescence - Anti-RBM19 antibody (ab122515)ab122515 at 4 µg/ml staining RBM19 in A431 cells by Immunofluorescence (green).
Note: positivity in nucleus but not nucleoli & cytoplasm. -
Western blot - Anti-RBM19 antibody (ab122515)All lanes : Anti-RBM19 antibody (ab122515) at 1/250 dilution
Lane 1 : RT4 lysate
Lane 2 : U251 MGlysate
Lane 3 : Human plasma
Lane 4 : Human liver lysate
Lane 5 : Human tonsil lysateDeveloped using the ECL technique
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab122515 被引用在 1 文献中.
- Kinterova V et al. Inhibition of SCF complexes during bovine oocyte maturation and preimplantation development leads to delayed development of embryos. Biol Reprod N/A:N/A (2018). PubMed: 30535233