Anti-RBM14抗体(ab70636)
Key features and details
- Rabbit polyclonal to RBM14
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-RBM14抗体
参阅全部 RBM14 一抗 -
描述
兔多克隆抗体to RBM14 -
宿主
Rabbit -
经测试应用
适用于: WB, IPmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Rat, Rabbit, Guinea pig, Cow, Dog, Pig, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Orangutan
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- Whole cell lysates from HeLa and 293T cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab70636于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (1) |
1/1000 - 1/5000. Detects a band of approximately 75 kDa (predicted molecular weight: 69 kDa).
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| IP |
Use at 2-5 µg/mg of lysate.
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| 说明 |
|---|
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WB
1/1000 - 1/5000. Detects a band of approximately 75 kDa (predicted molecular weight: 69 kDa). |
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IP
Use at 2-5 µg/mg of lysate. |
靶标
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功能
Isoform 1 may function as a nuclear receptor coactivator, enhancing transcription through other coactivators such as NCOA6 and CITED1. Isoform 2, functions as a transcriptional repressor, modulating transcriptional activities of coactivators including isoform 1, NCOA6 and CITED1 (PubMed:11443112). Regulates centriole biogenesis by suppressing the formation of aberrant centriolar protein complexes in the cytoplasm and thus preserving mitotic spindle integrity. Prevents the formation of the STIL-CENPJ complex (which can induce the formation of aberrant centriolar protein complexes) by interfering with the interaction of STIL with CENPJ (PubMed:25385835). -
组织特异性
Expressed in all tissues tested, including brain, heart, skeletal muscle, colon, thymus, spleen, kidney, liver, small intestine, placenta, lung and peripheral blood lymphocytes. -
序列相似性
Contains 2 RRM (RNA recognition motif) domains. -
细胞定位
Nucleus. Nucleus, nucleolus. Cytoplasm. In punctate subnuclear structures often located adjacent to splicing speckles, called paraspeckles (PubMed:11790299). Cytoplasmic localization is crucial for its function in suppressing the formation of aberrant centriolar protein complexes (PubMed:25385835). - Information by UniProt
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数据库链接
- Entrez Gene: 511512 Cow
- Entrez Gene: 100526737 Human
- Entrez Gene: 10432 Human
- Entrez Gene: 56275 Mouse
- Entrez Gene: 170900 Rat
- Omim: 612409 Human
- SwissProt: Q5EA36 Cow
- SwissProt: Q96PK6 Human
see all -
别名
- CoAA antibody
- Coactivator Activator antibody
- Paraspeckle protein 2 antibody
see all
图片
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Western blot - Anti-RBM14 antibody (ab70636)All lanes : Anti-RBM14 antibody (ab70636) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 15 µg
Lane 3 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Lane 4 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg
Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 50 µg
Predicted band size: 69 kDa
Exposure time: 30 seconds -
Immunoprecipitation - Anti-RBM14 antibody (ab70636)Detection of RBM14 by Western Blot of Immunprecipitate. ab70636 at 1µg/ml staining RBM14 in HeLa whole cell lysate immunoprecipitated using ab70636 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 30 seconds.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (13)
ab70636 被引用在 13 文献中.
- Su Y et al. Study of FOXO1-interacting proteins using TurboID-based proximity labeling technology. BMC Genomics 24:146 (2023). PubMed: 36964488
- Hu Y et al. RBM14 as a novel epigenetic-activated tumor oncogene is implicated in the reprogramming of glycolysis in lung cancer. World J Surg Oncol 21:132 (2023). PubMed: 37060064
- Vujovic A et al. In Vivo Screening Unveils Pervasive RNA-Binding Protein Dependencies in Leukemic Stem Cells and Identifies ELAVL1 as a Therapeutic Target. Blood Cancer Discov 4:180-207 (2023). PubMed: 36763002
- Narabayashi H et al. Gut microbiota-dependent adaptor molecule recruits DNA methyltransferase to the TLR4 gene in colonic epithelial cells to suppress inflammatory reactions. Front Mol Biosci 9:1005136 (2022). PubMed: 36339704
- Qin H et al. RBM14 Modulates Tubulin Acetylation and Regulates Spindle Morphology During Meiotic Maturation in Mouse Oocytes. Front Cell Dev Biol 9:635728 (2021). PubMed: 33604343