AB70636

Anti-RBM14 抗体

Name: RBM14抗体 (ab70636)| Abcam中文官网
Brand: Abcam Limited
SKU: AB70636
Price: 4744 CNY
Availability: InStock
Rating: 4 (2 reviews)

Anti-RBM14 antibody

(2 Reviews)

(18 Publications)

Rabbit Polyclonal RBM14 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 18 publications.

查看别名

SIP, RBM14, RNA-binding protein 14, Paraspeckle protein 2, RNA-binding motif protein 14, RRM-containing coactivator activator/modulator, Synaptotagmin-interacting protein, PSP2, SYT-interacting protein

2 Images

Unknown

Immunoprecipitation - Anti-RBM14 antibody (AB70636)

Detection of RBM14 by Western Blot of Immunprecipitate. ab70636 at 1µg/ml staining RBM14 in HeLa whole cell lysate immunoprecipitated using ab70636 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection : Chemiluminescence with exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-RBM14 antibody (ab70636)

Predicted band size: 69 kDa

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Supplier Data

Western blot - Anti-RBM14 antibody (AB70636)

All lanes:

Western blot - Anti-RBM14 antibody (ab70636) at 0.1 µg/mL

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 2:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 15 µg

Lane 3:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Lane 4:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg

Lane 5:

NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 50 µg

Predicted band size: 69 kDa

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Exposure time: 30s

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

反应种属

Mouse, Human

应用

IP, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Ferret": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Guinea pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Immunogen

存储溶液

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: PBS, 1.815% Tris, 1.764% Sodium citrate

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Isoform 1 may function as a nuclear receptor coactivator, enhancing transcription through other coactivators such as NCOA6 and CITED1. Isoform 2, functions as a transcriptional repressor, modulating transcriptional activities of coactivators including isoform 1, NCOA6 and CITED1 (PubMed : 11443112). Regulates centriole biogenesis by suppressing the formation of aberrant centriolar protein complexes in the cytoplasm and thus preserving mitotic spindle integrity. Prevents the formation of the STIL-CENPJ complex (which can induce the formation of aberrant centriolar protein complexes) by interfering with the interaction of STIL with CENPJ (PubMed : 25385835). Plays a role in the regulation of DNA virus-mediated innate immune response by assembling into the HDP-RNP complex, a complex that serves as a platform for IRF3 phosphorylation and subsequent innate immune response activation through the cGAS-STING pathway (PubMed : 28712728). Also involved in the regulation of pre-mRNA alternative splicing (PubMed : 37548402).

See full target information RBM14

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文献 (18)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 26: PubMed40650263

2025

Nuclear Retention of mRNAs Through Paraspeckle Protein Binding to a Sequence Determinant in 3'UTR.

Applications

Unspecified application

Species

Unspecified reactive species

Audrey Jacq,Denis Becquet,Bénédicte Boyer,Séverine Guillen,Maria-Montserrat Bello-Goutierrez,Marie-Pierre Blanchard,Claude Villard,Maya Belghazi,Manon Torres,Jean-Louis Franc,Anne-Marie François-Bellan

Nature communications 16:1077 PubMed39870664

2025

A compendium of Amplification-Related Gain Of Sensitivity genes in human cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Veronica Rendo,Michael Schubert,Nicholas Khuu,Maria F Suarez Peredo Rodriguez,Declan Whyte,Xiao Ling,Anouk van den Brink,Kaimeng Huang,Michelle Swift,Yizhou He,Johanna Zerbib,Ross Smith,Jonne Raaijmakers,Pratiti Bandopadhayay,Lillian M Guenther,Justin H Hwang,Amanda Iniguez,Susan Moody,Ji-Heui Seo,Elizabeth H Stover,Levi Garraway,William C Hahn,Kimberly Stegmaier,René H Medema,Dipanjan Chowdhury,Maria Colomé-Tatché,Uri Ben-David,Rameen Beroukhim,Floris Foijer

Nucleic acids research 51:12242-12260 PubMed37930826

2023

The RNA-binding motif protein 14 regulates telomere integrity at the interface of TERRA and telomeric R-loops.

Applications

Unspecified application

Species

Unspecified reactive species

Yajun Wang,Wei Zhu,Yumi Jang,Joshua A Sommers,Gong Yi,Chandrakala Puligilla,Deborah L Croteau,Yibin Yang,Mihoko Kai,Yie Liu

World journal of surgical oncology 21:132 PubMed37060064

2023

RBM14 as a novel epigenetic-activated tumor oncogene is implicated in the reprogramming of glycolysis in lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Hu,Hanshuo Mu,Zhiping Deng

BMC genomics 24:146 PubMed36964488

2023

Study of FOXO1-interacting proteins using TurboID-based proximity labeling technology.

Applications

Unspecified application

Species

Unspecified reactive species

Yanting Su,Yuanyuan Guo,Jieyu Guo,Ting Zeng,Ting Wang,Wu Liu

Blood cancer discovery 4:180-207 PubMed36763002

2023

In Vivo Screening Unveils Pervasive RNA-Binding Protein Dependencies in Leukemic Stem Cells and Identifies ELAVL1 as a Therapeutic Target.

Applications

Unspecified application

Species

Unspecified reactive species

Ana Vujovic,Laura de Rooij,Ava Keyvani Chahi,He Tian Chen,Brian A Yee,Sampath K Loganathan,Lina Liu,Derek C H Chan,Amanda Tajik,Emily Tsao,Steven Moreira,Pratik Joshi,Joshua Xu,Nicholas Wong,Zaldy Balde,Soheil Jahangiri,Sasan Zandi,Stefan Aigner,John E Dick,Mark D Minden,Daniel Schramek,Gene W Yeo,Kristin J Hope

Frontiers in molecular biosciences 9:1005136 PubMed36339704

2022

Gut microbiota-dependent adaptor molecule recruits DNA methyltransferase to the TLR4 gene in colonic epithelial cells to suppress inflammatory reactions.

Applications

Unspecified application

Species

Unspecified reactive species

Hikari Narabayashi,Chiharu Koma,Kazuaki Nakata,Mion Ikegami,Yusuke Nakanishi,Jun Ogihara,Masato Tsuda,Akira Hosono,Shigemasa Hanazawa,Kyoko Takahashi

Journal of oncology 2022:5091154 PubMed35669242

2022

Solute Carrier Family 35 Member F2 Regulates Cisplatin Resistance and Promotes Malignant Progression of Pancreatic Cancer by Regulating RNA Binding Motif Protein 14.

Applications

Unspecified application

Species

Unspecified reactive species

Shuxian Zhang,Qingqing Li,Huixiao Yuan,Ling Ren,Xuyang Liang,Shouying Li,Shengxiang Lv,Hua Jiang

Frontiers in cell and developmental biology 9:635728 PubMed33604343

2021

RBM14 Modulates Tubulin Acetylation and Regulates Spindle Morphology During Meiotic Maturation in Mouse Oocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Hao Qin,Yi Qu,Yi-Feng Yuan,Yang-Yang Li,Jie Qiao

Proceedings of the National Academy of Sciences of 117:5329-5338 PubMed32094185

2020

Intrinsically disordered protein RBM14 plays a role in generation of RNA:DNA hybrids at double-strand break sites.

Applications

Unspecified application

Species

Unspecified reactive species

Yumi Jang,Zeinab Elsayed,Rebeka Eki,Shuaixin He,Kang-Ping Du,Tarek Abbas,Mihoko Kai

View all publications

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Anti-RBM14 抗体

Anti-RBM14 antibody

Immunoprecipitation - Anti-RBM14 antibody (AB70636)

Western blot - Anti-RBM14 antibody (AB70636)