Anti-RBBP4抗体(ab38135)
Key features and details
- Rabbit polyclonal to RBBP4
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-RBBP4抗体
参阅全部 RBBP4 一抗 -
描述
兔多克隆抗体to RBBP4 -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, WBmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Chicken, Cow, Xenopus laevis, Zebrafish
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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ChIP Related Products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab38135于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
Use a concentration of 1 µg/ml.
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| WB | (1) |
1/250. Detects a band of approximately 49 kDa (predicted molecular weight: 48 kDa).
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| 说明 |
|---|
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ICC/IF
Use a concentration of 1 µg/ml. |
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WB
1/250. Detects a band of approximately 49 kDa (predicted molecular weight: 48 kDa). |
靶标
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功能
Core histone-binding subunit that may target chromatin assembly factors, chromatin remodeling factors and histone deacetylases to their histone substrates in a manner that is regulated by nucleosomal DNA. Component of several complexes which regulate chromatin metabolism. These include the chromatin assembly factor 1 (CAF-1) complex, which is required for chromatin assembly following DNA replication and DNA repair; the core histone deacetylase (HDAC) complex, which promotes histone deacetylation and consequent transcriptional repression; the nucleosome remodeling and histone deacetylase complex (the NuRD complex), which promotes transcriptional repression by histone deacetylation and nucleosome remodeling; the PRC2/EED-EZH2 complex, which promotes repression of homeotic genes during development; and the NURF (nucleosome remodeling factor) complex. -
序列相似性
Belongs to the WD repeat RBAP46/RBAP48/MSI1 family.
Contains 6 WD repeats. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 395658 Chicken
- Entrez Gene: 767940 Cow
- Entrez Gene: 5928 Human
- Entrez Gene: 19646 Mouse
- Entrez Gene: 321726 Zebrafish
- Omim: 602923 Human
- SwissProt: Q9W7I5 Chicken
- SwissProt: Q3MHL3 Cow
see all -
别名
- CAF I p48 antibody
- CAF-1 subunit C antibody
- CAF-I 48 kDa subunit antibody
see all
图片
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Western blot - Anti-RBBP4 antibody (ab38135)All lanes : Anti-RBBP4 antibody (ab38135) at 1/250 dilution
Lane 1 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 3 :Jurkat whole cell lysate (ab7899)
Lane 4 :A-431 whole cell lysate (ab7909)
Lane 5 :HEK-293 whole cell lysate (ab7902)
Lane 6 : Hep G2 whole cell lysate (ab7900)
Lane 7 : MCF-7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 48 kDa
Observed band size: 49 kDa why is the actual band size different from the predicted?
Additional bands at: 100 kDa. We are unsure as to the identity of these extra bands. -
Immunocytochemistry/ Immunofluorescence - Anti-RBBP4 antibody (ab38135)ICC/IF image of ab38135 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab38135, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab38135 被引用在 3 文献中.
- Muralidharan B et al. LHX2 Interacts with the NuRD Complex and Regulates Cortical Neuron Subtype Determinants Fezf2 and Sox11. J Neurosci 37:194-203 (2017). ChIP-sequencing, IP ; Mouse . PubMed: 28053041
- Gu X et al. Runx1 regulation of Pu.1 corepressor/coactivator exchange identifies specific molecular targets for leukemia differentiation therapy. J Biol Chem 289:14881-95 (2014). PubMed: 24695740
- Fujita T & Fujii H Identification of proteins associated with an IFN?-responsive promoter by a retroviral expression system for enChIP using CRISPR. PLoS One 9:e103084 (2014). ChIP ; Human . PubMed: 25051498