Anti-RBBP4抗体(ab1765)
Key features and details
- Rabbit polyclonal to RBBP4
- Suitable for: ICC/IF, IHC-P, IP, WB
- Reacts with: Mouse, Dog, Human
- Isotype: IgG
概述
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产品名称
Anti-RBBP4抗体
参阅全部 RBBP4 一抗 -
描述
兔多克隆抗体to RBBP4 -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, IHC-P, IP, WBmore details -
种属反应性
与反应: Mouse, Dog, Human
预测可用于: Chicken, Xenopus laevis
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免疫原
Synthetic peptide corresponding to Human RBBP4 aa 406-425.
Sequence:CENIYNDEDPEGSVDPEGQGS
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常规说明
Locus link: 350005 Alternative names: similar to chromatin assembly factor 1 p48 subunitA protein complex that is thought to mediate chromatin assembly in DNA replication and DNA repair. Assembles histone octamers onto replicating DNA in vitro. CAF-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA; Histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer. P48 can bind to histone H4 in the absence of CAF-1 P150 and P160. Binds directly to helix 1 of histone H4, a region that is not accessible when H4 is in chromatin. SUBUNIT: CAF-1 is composed of three subunits, P48, P60 AND P150. Only minor amounts of P48 are complexed with P60 AND P150 in G1 phase. P48/RBBP4 is also part of the core histone deacetylase (HDAC) complex composed of HDAC1, HDAC2, RBBP4 and RBBP7. The core complex associates with MTA2, MBD3, MTA1L1, CHD3 and CHD4 to form the nucleosome remodelling and histone deacetylation (NuRD) complex, or with SIN3, SAP18 and SAP30 to form the SIN3 HDAC complex. Interacts with the viral protein-binding domain of the retinoblastoma protein (RB1). Interacts with SUV39H1 and HDAC7 (By similarity).
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.02% Sodium azide -
Concentration information loading... -
纯度
Whole antiserum -
Primary antibody说明
A protein complex that is thought to mediate chromatin assembly in DNA replication and DNA repair. Assembles histone octamers onto replicating DNA in vitro. CAF-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA; Histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer. P48 can bind to histone H4 in the absence of CAF-1 P150 and P160. Binds directly to helix 1 of histone H4, a region that is not accessible when H4 is in chromatin. SUBUNIT: CAF-1 is composed of three subunits, P48, P60 AND P150. Only minor amounts of P48 are complexed with P60 AND P150 in G1 phase. P48/RBBP4 is also part of the core histone deacetylase (HDAC) complex composed of HDAC1, HDAC2, RBBP4 and RBBP7. The core complex associates with MTA2, MBD3, MTA1L1, CHD3 and CHD4 to form the nucleosome remodelling and histone deacetylation (NuRD) complex, or with SIN3, SAP18 and SAP30 to form the SIN3 HDAC complex. Interacts with the viral protein-binding domain of the retinoblastoma protein (RB1). Interacts with SUV39H1 and HDAC7 (By similarity). -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | (2) |
Use at an assay dependent concentration.
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| IHC-P | (2) |
Use at an assay dependent concentration.
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| IP | (1) |
Use at an assay dependent concentration.
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| WB | (3) |
1/1000. Detects a band of approximately 55 kDa (predicted molecular weight: 47.6 kDa).
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| 说明 |
|---|
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ICC/IF
Use at an assay dependent concentration. |
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IHC-P
Use at an assay dependent concentration. |
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IP
Use at an assay dependent concentration. |
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WB
1/1000. Detects a band of approximately 55 kDa (predicted molecular weight: 47.6 kDa). |
靶标
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功能
Core histone-binding subunit that may target chromatin assembly factors, chromatin remodeling factors and histone deacetylases to their histone substrates in a manner that is regulated by nucleosomal DNA. Component of several complexes which regulate chromatin metabolism. These include the chromatin assembly factor 1 (CAF-1) complex, which is required for chromatin assembly following DNA replication and DNA repair; the core histone deacetylase (HDAC) complex, which promotes histone deacetylation and consequent transcriptional repression; the nucleosome remodeling and histone deacetylase complex (the NuRD complex), which promotes transcriptional repression by histone deacetylation and nucleosome remodeling; the PRC2/EED-EZH2 complex, which promotes repression of homeotic genes during development; and the NURF (nucleosome remodeling factor) complex. -
序列相似性
Belongs to the WD repeat RBAP46/RBAP48/MSI1 family.
Contains 6 WD repeats. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 395658 Chicken
- Entrez Gene: 5928 Human
- Entrez Gene: 19646 Mouse
- Omim: 602923 Human
- SwissProt: Q9W7I5 Chicken
- SwissProt: Q09028 Human
- SwissProt: Q60972 Mouse
- Unigene: 16003 Human
see all -
别名
- CAF I p48 antibody
- CAF-1 subunit C antibody
- CAF-I 48 kDa subunit antibody
see all
图片
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Western blot - Anti-RBBP4 antibody (ab1765)Lane 1 : Anti-RBBP4 antibody (ab1765) at 1/500 dilution
Lane 2 : Anti-RBBP4 antibody (ab1765) at 1/1000 dilution
All lanes : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/2000 dilution
Predicted band size: 47.6 kDa -
Western blot - Anti-RBBP4 antibody (ab1765)Anti-RBBP4 antibody (ab1765) at 1/1000 dilution + Human LCL whole cell lysate
Secondary
Anti-rabbit IgG HRP
Developed using the ECL technique.
Predicted band size: 47.6 kDa
Observed band size: 40,55 kDa why is the actual band size different from the predicted?
Exposure time: 1 minute
数据表及文件
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SDS download
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Datasheet download
文献 (14)
ab1765 被引用在 14 文献中.
- Bakail M et al. Design on a Rational Basis of High-Affinity Peptides Inhibiting the Histone Chaperone ASF1. Cell Chem Biol 26:1573-1585.e10 (2019). PubMed: 31543461
- Li D et al. Armadillo repeat containing 12 promotes neuroblastoma progression through interaction with retinoblastoma binding protein 4. Nat Commun 9:2829 (2018). PubMed: 30026490
- Jin X et al. Overexpression of retinoblastoma-binding protein 4 contributes to the radiosensitivity of AGS gastric cancer cells via phosphoinositide3-kinase/protein kinase B pathway suppression. Mol Med Rep 18:1571-1581 (2018). PubMed: 29901205
- Cohen C et al. Promyelocytic leukemia (PML) nuclear bodies (NBs) induce latent/quiescent HSV-1 genomes chromatinization through a PML NB/Histone H3.3/H3.3 Chaperone Axis. PLoS Pathog 14:e1007313 (2018). PubMed: 30235352
- Sun D et al. LncRNA GAS5 inhibits microglial M2 polarization and exacerbates demyelination. EMBO Rep N/A:N/A (2017). PubMed: 28808113