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Cell Biology Cell Cycle Cell Cycle Inhibitors Rb

Anti-Rb (phospho T821)抗体(ab4787)

  • Datasheet
  • SDS
Reviews (1)Q&A (1)References (11)

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Western blot - Anti-Rb (phospho T821) antibody (ab4787)

    Key features and details

    • Rabbit polyclonal to Rb (phospho T821)
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

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    概述

    • 产品名称

      Anti-Rb (phospho T821)抗体
      参阅全部 Rb 一抗
    • 描述

      兔多克隆抗体to Rb (phospho T821)
    • 宿主

      Rabbit
    • 经测试应用

      适用于: WBmore details
    • 种属反应性

      与反应: Human
      预测可用于: Rat
    • 免疫原

      Synthetic peptide corresponding to Human Rb (phospho T821).
      Database link: P06400

    • 常规说明

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    性能

    • 形式

      Liquid
    • 存放说明

      Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
    • 存储溶液

      pH: 7.30
      Preservative: 0.05% Sodium azide
      Constituents: PBS, 50% Glycerol, 0.1% BSA
    • Concentration information loading...
    • 纯度

      Immunogen affinity purified
    • 纯化说明

      Purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated Rb protein. The final product is generated by affinity chromatography using a Rb-derived peptide that is phosphorylated at threonine 821.
    • 克隆

      多克隆
    • 同种型

      IgG
    • 研究领域

      • Cell Biology
      • Cell Cycle
      • Cell Cycle Inhibitors
      • Rb
      • Epigenetics and Nuclear Signaling
      • Transcription
      • Cancer susceptibility
      • Tumor Suppressors
      • Cancer
      • Oncoproteins/suppressors
      • Tumor suppressors
      • Rb family

    相关产品

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Positive Controls

      • Jurkat whole cell lysate (ab7899)
    • Recombinant Protein

      • Recombinant Human Rb protein (ab83205)

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab4787于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    WB
    1/1000. Detects a band of approximately 120 kDa (predicted molecular weight: 106 kDa).
    说明
    WB
    1/1000. Detects a band of approximately 120 kDa (predicted molecular weight: 106 kDa).

    靶标

    • 功能

      Key regulator of entry into cell division that acts as a tumor suppressor. Promotes G0-G1 transition when phosphorylated by CDK3/cyclin-C. Acts as a transcription repressor of E2F1 target genes. The underphosphorylated, active form of RB1 interacts with E2F1 and represses its transcription activity, leading to cell cycle arrest. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV39H1, KMT5B and KMT5C, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Inhibits the intrinsic kinase activity of TAF1. Mediates transcriptional repression by SMARCA4/BRG1 by recruiting a histone deacetylase (HDAC) complex to the c-FOS promoter. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex (By similarity). In case of viral infections, interactions with SV40 large T antigen, HPV E7 protein or adenovirus E1A protein induce the disassembly of RB1-E2F1 complex thereby disrupting RB1's activity.
    • 组织特异性

      Expressed in the retina.
    • 疾病相关

      Childhood cancer retinoblastoma
      Bladder cancer
      Osteogenic sarcoma
    • 序列相似性

      Belongs to the retinoblastoma protein (RB) family.
    • 结构域

      The Pocket domain binds to the threonine-phosphorylated domain C, thereby preventing interaction with heterodimeric E2F/DP transcription factor complexes.
    • 翻译后修饰

      Phosphorylated by CDK6 and CDK4, and subsequently by CDK2 at Ser-567 in G1, thereby releasing E2F1 which is then able to activate cell growth. Dephosphorylated at the late M phase. SV40 large T antigen, HPV E7 and adenovirus E1A bind to the underphosphorylated, active form of pRb. Phosphorylation at Thr-821 and Thr-826 promotes interaction between the C-terminal domain C and the Pocket domain, and thereby inhibits interactions with heterodimeric E2F/DP transcription factor complexes. Dephosphorylated at Ser-795 by calcineruin upon calcium stimulation. CDK3/cyclin-C-mediated phosphorylation at Ser-807 and Ser-811 is required for G0-G1 transition. Phosphorylated by CDK1 and CDK2 upon TGFB1-mediated apoptosis.
      N-terminus is methylated by METTL11A/NTM1 (By similarity). Monomethylation at Lys-810 by SMYD2 enhances phosphorylation at Ser-807 and Ser-811, and promotes cell cycle progression. Monomethylation at Lys-860 by SMYD2 promotes interaction with L3MBTL1.
      Acetylation at Lys-873 and Lys-874 regulates subcellular localization, at least during keratinocytes differentiation.
    • 细胞定位

      Nucleus.
    • Target information above from: UniProt accession P06400 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • 数据库链接

      • Entrez Gene: 5925 Human
      • Entrez Gene: 24708 Rat
      • Omim: 614041 Human
      • SwissProt: P06400 Human
      • SwissProt: P33568 Rat
      • Unigene: 408528 Human
      • Unigene: 55115 Rat
      • 别名

        • Exon 17 tumor GOS561 substitution mutation causes premature stop antibody
        • GOS563 exon 17 substitution mutation causes premature stop antibody
        • OSRC antibody
        • Osteosarcoma antibody
        • p105-Rb antibody
        • P105RB antibody
        • PP105 antibody
        • pp110 antibody
        • PPP1R130 antibody
        • pRb antibody
        • Prepro retinoblastoma associated protein antibody
        • Protein phosphatase 1 regulatory subunit 130 antibody
        • Rb antibody
        • RB transcriptional corepressor 1 antibody
        • RB_HUMAN antibody
        • RB1 antibody
        • RB1 gene antibody
        • Retinoblastoma 1 antibody
        • Retinoblastoma suspectibility protein antibody
        • Retinoblastoma-associated protein antibody
        see all

      图片

      • Western blot - Anti-Rb (phospho T821) antibody (ab4787)
        Western blot - Anti-Rb (phospho T821) antibody (ab4787)
        Peptide Competition: Cell extracts prepared from Jurkat cells in high growth phase were resolved by SDS-PAGE on a 10% Tris-glycine gel and transferred to PVDF. Membranes were incubated with 0.50 µg/mL ab4787, following prior incubation in the: absence of the phosphopeptide immunogen (1), a generic phosphothreonine containing peptide (2), the non phosphopeptide corresponding to the phosphopeptide immunogen (3), or the presence of the phosphopeptide immunogen (4). After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG alkaline phosphatase and bands were detected using the Tropix WesternStar detection method. The data show that only the phosphopeptide corresponding to this site blocks the antibody signal, demonstrating the specificity of the ab4787 antibody for this epitope. Peptide Competition: Cell extracts prepared from Jurkat cells in high growth phase were resolved by SDS-PAGE on a 10% Tris-glycine gel and transferred to PVDF. Membranes were in

      实验方案

      • Peptide Competition Experiment
      • Western blotting protocol

      Click here to view the general protocols

      数据表及文件

      • SDS download

      • Datasheet download

        Download

      文献 (11)

      发表研究结果有使用 ab4787?请让我们知道,以便我们可以引用本数据表中的参考文章。

      ab4787 被引用在 11 文献中.

      • Kaminska K  et al. Distinct mechanisms of resistance to fulvestrant treatment dictate level of ER independence and selective response to CDK inhibitors in metastatic breast cancer. Breast Cancer Res 23:26 (2021). PubMed: 33602273
      • Adhikari A & Davie JK The PRC2 complex directly regulates the cell cycle and controls proliferation in skeletal muscle. Cell Cycle 19:2373-2394 (2020). PubMed: 32816597
      • Martinez-Soria N  et al. The Oncogenic Transcription Factor RUNX1/ETO Corrupts Cell Cycle Regulation to Drive Leukemic Transformation. Cancer Cell 34:626-642.e8 (2018). PubMed: 30300583
      • Patel H  et al. ICEC0942, an Orally Bioavailable Selective Inhibitor of CDK7 for Cancer Treatment. Mol Cancer Ther 17:1156-1166 (2018). PubMed: 29545334
      • Liao YX  et al. Effects of Fibronectin 1 on Cell Proliferation, Senescence and Apoptosis of Human Glioma Cells Through the PI3K/AKT Signaling Pathway. Cell Physiol Biochem 48:1382-1396 (2018). PubMed: 30048971
      View all Publications for this product

      客户评价及客户问答

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      1-2 of 2 Abreviews or Q&A

      Immunocytochemistry/ Immunofluorescence abreview for Anti-Rb (phospho T821) antibody

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Immunocytochemistry/ Immunofluorescence
      Blocking step
      Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2.5% · Temperature: RT°C
      Sample
      Mouse Cell (B cell line)
      Specification
      B cell line
      Permeabilization
      Yes - 0,1% TritonX-100/PBS for 10 minutes at RT
      Fixative
      Paraformaldehyde
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      提交于 Jun 03 2014

      Question

      Hi there, I was wondering if you could let me know if you have any pure positive controls or blocking peptides for either of these two antibodies: Anti-Phospho Rb (T821) #ab4787 Anti-Rb (ab6075) For both there are suggested cell lysates to use as positive controls, but I need a pure protein to verify the exact band on my western blots. I know you provide a His-tagged version of the Rb full length protein, but do you have any non-tagged versions? Or do you know a good vendor for these? Also, the blocking peptide for phospho-Rb is no longer available (#ab5324); is there another source I could use to get this blocking peptide?

      Read More

      Abcam community

      Verified customer

      Asked on Sep 14 2011

      Answer

      Thank you for contacting us. We currently only have the tagged Rb proteins in our catalog and no blocking peptide for ab4787 is available. I would sugges Biocompare.com to search for these products. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

      Read More

      Abcam Scientific Support

      回复于 Sep 14 2011

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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