重组Anti-RASGRP3 (phospho T133)抗体[EPNCIR148] (ab124823)
![Western blot - Anti-RASGRP3 (phospho T133) antibody [EPNCIR148] (ab124823)](https://www.abcam.cn/ps/products/124/ab124823/Images/ab124823-138751-anti-rasgrp3-phospho-t133-antibody-epncir148-western-blot.jpg "Western blot - Anti-RASGRP3 (phospho T133) antibody [EPNCIR148] (ab124823)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPNCIR148] to RASGRP3 (phospho T133)
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-RASGRP3 (phospho T133)抗体[EPNCIR148]
参阅全部 RASGRP3 一抗 -
描述
兔单克隆抗体[EPNCIR148] to RASGRP3 (phospho T133) -
宿主
Rabbit -
经测试应用
适用于: WBmore details
不适用于: IHC-P or IP -
种属反应性
与反应: Human
预测可用于: Mouse
不与反应:
Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- Ramos cell lysates
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常规说明
This antibody was developed as part of a collaboration between the National Cancer Institute's Center for Cancer Research and the lab of Peter Blumberg. View antibodies from NCI Center for Cancer Research Collaboration.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPNCIR148 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab124823于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 78 kDa.
|
| 说明 |
|---|
|
WB
1/1000 - 1/10000. Predicted molecular weight: 78 kDa. |
应用说明
Is unsuitable for IHC-P or IP.
靶标
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功能
Guanine nucleotide exchange factor (GEF) for Ras and Rap1. -
序列相似性
Belongs to the RASGRP family.
Contains 2 EF-hand domains.
Contains 1 N-terminal Ras-GEF domain.
Contains 1 phorbol-ester/DAG-type zinc finger.
Contains 1 Ras-GEF domain. - Information by UniProt
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数据库链接
- Entrez Gene: 25780 Human
- Entrez Gene: 240168 Mouse
- Omim: 609531 Human
- SwissProt: Q8IV61 Human
- Unigene: 143674 Human
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别名
- Calcium and DAG-regulated guanine nucleotide exchange factor III antibody
- Grp3 antibody
- GRP3_HUMAN antibody
see all
图片
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Western blot - Anti-RASGRP3 (phospho T133) antibody [EPNCIR148] (ab124823)All lanes : Anti-RASGRP3 (phospho T133) antibody [EPNCIR148] (ab124823) at 1/1000 dilution
Lane 1 : Ramos cell lysate, untreated
Lane 2 : Ramos cell lysate treated with Lambda Phosphatase
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled Goat anti-Rabbit at 1/2000 dilution
Predicted band size: 78 kDa -
![Anti-RASGRP3 (phospho T133) antibody [EPNCIR148] (ab124823)](https://www.abcam.cn/ps/products/124/ab124823/Images/ab124823-3-benefits-of-recombinant-antibodies.png)
Anti-RASGRP3 (phospho T133) antibody [EPNCIR148] (ab124823)
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab124823 被引用在 4 文献中.
- Yang JF et al. SOX9 promotes the invasion and migration of lung adenocarcinoma cells by activating the RAP1 signaling pathway. BMC Pulm Med 23:421 (2023). PubMed: 37919693
- Tang S et al. RasGRP3 limits Toll-like receptor-triggered inflammatory response in macrophages by activating Rap1 small GTPase. Nat Commun 5:4657 (2014). WB . PubMed: 25118589
- Nagy Z et al. Function of RasGRP3 in the formation and progression of human breast cancer. Mol Cancer 13:96 (2014). PubMed: 24779681
- Song X et al. RasGRPs are targets of the anti-cancer agent ingenol-3-angelate. PLoS One 8:e72331 (2013). WB ; Rat . PubMed: 23991094