Anti-RASGRF2抗体(ab121577)
Key features and details
- Rabbit polyclonal to RASGRF2
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-RASGRF2抗体
参阅全部 RASGRF2 一抗 -
描述
兔多克隆抗体to RASGRF2 -
宿主
Rabbit -
经测试应用
适用于: IHC-P, ICC/IF, WBmore details -
种属反应性
与反应: Human -
免疫原
antigen, corresponding to internal sequence amino acids 702-807 of Human RASGRF2.
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阳性对照
- Human colon tissue; Human cell line A-431; Human liver, Human tonsil, RT-4, and U-251 MG cell lysates.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab121577于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Use buffer at pH 6 for antigen retrieval.
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| ICC/IF |
Use a concentration of 1 - 4 µg/ml. Fixation/Permeabilization: PFA/Triton X-100
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| WB |
1/250 - 1/500.
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| 说明 |
|---|
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IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Use buffer at pH 6 for antigen retrieval. |
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ICC/IF
Use a concentration of 1 - 4 µg/ml. Fixation/Permeabilization: PFA/Triton X-100 |
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WB
1/250 - 1/500. |
靶标
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功能
Functions as a calcium-regulated nucleotide exchange factor activating both Ras and RAC1 through the exchange of bound GDP for GTP. Preferentially activates HRAS in vivo compared to RRAS based on their different types of prenylation. Functions in synaptic plasticity by contributing to the induction of long term potentiation. -
组织特异性
Widely expressed with higher expression in brain, followed by heart, lung, pancreas and kidney. Detected in placenta. Expressed in brain and lung (at protein level). -
序列相似性
Contains 1 DH (DBL-homology) domain.
Contains 1 IQ domain.
Contains 1 N-terminal Ras-GEF domain.
Contains 2 PH domains.
Contains 1 Ras-GEF domain. -
结构域
The Ras-GEF domain and the N-terminal Ras-GEF domain form a Ras-binding site and mediate Ras activation.
The IQ domain mediates the calcium-dependent interaction with calmodulin but is dispensable for the Ras-GEF activity.
The DH (DBL-homology) domain mediates interaction with RASGRF1 and EPB49 and is required for RAC1 activation. -
翻译后修饰
Phosphorylated by CDK5; down-regulates RASGRF2-mediated RAC1 activation.
Ubiquitinated upon interaction with Ras. Ubiquitination leads to degradation through the 26S proteasome. -
细胞定位
Cytoplasm. Cell membrane. Endoplasmic reticulum membrane. Translocates to membranes when activated. Found both at cell periphery and along the axon of neurons. - Information by UniProt
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数据库链接
- Entrez Gene: 5924 Human
- Omim: 606614 Human
- SwissProt: O14827 Human
- Unigene: 162129 Human
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别名
- GRF2 antibody
- Ras guanine nucleotide exchange factor 2 antibody
- Ras protein specific guanine nucleotide releasing factor 2 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RASGRF2 antibody (ab121577)
Immunohistochemical analysis of human testis tissue labelled for RASGRF2 using ab121577. Positive cytoplasmic staining is observed in cells in the seminiferous ducts.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RASGRF2 antibody (ab121577)Immunohistochemical analysis of human prostate tissue labelled for RASGRF2 using ab121577. Positive cytoplasmic staining is observed in glandular cells.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RASGRF2 antibody (ab121577)Immunohistochemical analysis of human cerebral cortex tissue labelled for RASGRF2 using ab121577. Positive cytoplasmic staining is observed in neuronal cells.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RASGRF2 antibody (ab121577)Immunohistochemical analysis of human placenta tissue labelled for RASGRF2 using ab121577. Positive cytoplasmic staining is observed in trophoblastic cells.
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Western blot - Anti-RASGRF2 antibody (ab121577)All lanes : Anti-RASGRF2 antibody (ab121577) at 1/250 dilution
Lane 1 : RT-4 cell lysate
Lane 2 : U-251 MG cell lysate
Lane 3 : Human plasma
Lane 4 : Human liver lysate
Lane 5 : Human tonsil lysate
Developed using the ECL technique. -
Immunocytochemistry/ Immunofluorescence - Anti-RASGRF2 antibody (ab121577)ab121577, at 4 µg/ml, staining RASGRF2 in PFA/Triton X-100 treated Human cell line A-431 by immunofluorescence. ab121577 staining is shown in green.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab121577 被引用在 4 文献中.
- Houlier A et al. RASGRF2 gene fusions identified in a variety of melanocytic lesions with distinct morphological features. Pigment Cell Melanoma Res 34:1074-1083 (2021). PubMed: 34310073
- Du Y et al. High Expression of ERK-related RASGRF2 predicts Poor prognosis in patients with Stomach Adenocarcinoma and correlates with M2 macrophage. J Cancer 12:7177-7189 (2021). PubMed: 34729119
- Korolkova OY et al. Reciprocal expression of Annexin A6 and RasGRF2 discriminates rapidly growing from invasive triple negative breast cancer subsets. PLoS One 15:e0231711 (2020). PubMed: 32298357
- Lu P et al. RasGRF2 promotes migration and invasion of colorectal cancer cells by modulating expression of MMP9 through Src/Akt/NF-?B pathway. Cancer Biol Ther 20:435-443 (2019). PubMed: 30359168