AB109465

重组Anti-RASA1 (phospho Y460)抗体[EPR2620(2)]

Anti-RASA1 (phospho Y460) antibody [EPR2620(2)]

RabMAb
Recombinant
了解详情

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(1 Publication)

Rabbit Recombinant Monoclonal RASA1 phospho Y460 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication.

查看别名

GAP, RASA, RASA1, Ras GTPase-activating protein 1, GTPase-activating protein, RasGAP, Ras p21 protein activator, p120GAP

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-RASA1 (phospho Y460) antibody [EPR2620(2)] (AB109465)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-RASA1 (phospho Y460) antibody [EPR2620(2)] (AB109465)

ab109465 at 1/100 dilution staining GAP in 293T cells by Immunofluorescence.

Unknown

Western blot - Anti-RASA1 (phospho Y460) antibody [EPR2620(2)] (AB109465)

Note : The lower panel utilized an alternative anti-GAP antibody which does not require phosphorylation for detection of the antigen.

All lanes:

Western blot - Anti-RASA1 (phospho Y460) antibody [EPR2620(2)] (ab109465) at 1/1000 dilution

Lane 1:

293T cell lysates, untreated at 10 µg

Lane 2:

293T cell lysates treated with Pervanadate at 10 µg

Secondary

All lanes:

HRP-labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 116 kDa

false

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR2620(2)

亚型

IgG

不含载体蛋白

反应种属

Human

应用

WB, ICC/IF

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

特异性

ab109465 only detects GAP phosphorylated at Tyrosine 460.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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产品详情

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Blue Ice

储存信息

Stable for 12 months at -20°C

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Inhibitory regulator of the Ras-cyclic AMP pathway. Stimulates the GTPase of normal but not oncogenic Ras p21; this stimulation may be further increased in the presence of NCK1.

See full target information RASA1 phospho Y460

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

PloS one 11:e0147758 PubMed26812085

2016

Cdc42 and k-Ras Control Endothelial Tubulogenesis through Apical Membrane and Cytoskeletal Polarization: Novel Stimulatory Roles for GTPase Effectors, the Small GTPases, Rac2 and Rap1b, and Inhibitory Influence of Arhgap31 and Rasa1.

Applications

Unspecified application

Species

Unspecified reactive species

Pieter R Norden,Dae Joong Kim,David M Barry,Ondine B Cleaver,George E Davis

View all publications

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Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。

详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

重组Anti-RASA1 (phospho Y460)抗体[EPR2620(2)]

Anti-RASA1 (phospho Y460) antibody [EPR2620(2)]

Immunocytochemistry/ Immunofluorescence - Anti-RASA1 (phospho Y460) antibody [EPR2620(2)] (AB109465)

Western blot - Anti-RASA1 (phospho Y460) antibody [EPR2620(2)] (AB109465)

关键信息

宿主种属

克隆

克隆号

亚型

不含载体蛋白

反应种属

应用

免疫原

特异性