AB16907

Anti-Ras 抗体 [F132-62]

Name: Ras抗体[F132-62] (ab16907)| Abcam中文官网
Brand: Abcam Limited
SKU: AB16907
Price: 6182 CNY
Availability: InStock
Rating: 3 (6 reviews)

Anti-Ras antibody [F132-62]

(6 Reviews)

(12 Publications)

Mouse Monoclonal RASH antibody. Suitable for Flow Cyt and reacts with Human samples. Cited in 12 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human HRAS.

查看别名

HRAS1, HRAS, GTPase HRas, H-Ras-1, Ha-Ras, Transforming protein p21, c-H-ras, p21ras

1 Images

Flow Cytometry - Anti-Ras antibody [F132-62] (AB16907)

Flow Cyt

Unknown

Flow Cytometry - Anti-Ras antibody [F132-62] (AB16907)

Overlay histogram showing HeLa cells stained with ab16907 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab16907, 1μg/1x10⁶ cells) for 30 min at 22°C. The secondary antibody used was DyLight^® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2μg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 100% methanol (5 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

This image was generated using the ascites version of the product.

关键信息

宿主种属

Mouse

克隆

Monoclonal

克隆号

F132-62

亚型

IgG2b

不含载体蛋白

反应种属

Human

应用

Flow Cyt

applications

免疫原

Recombinant Full Length Protein corresponding to Human HRAS.

P01112

特异性

Reactive with the Ras translational products of the H-, K- and N-ras human, as well as mouse and rat c- and v-ras oncogenes. We have received mixed results for Western Blot.

style

left-column

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p><a href='/products/primary-antibodies/mouse-igg2b-kappa-monoclonal-7e10g10-isotype-control-ab170192'>ab170192</a> - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.</p>" } } }

style

left-column

产品详情

This product was changed from ascites to tissue culture supernatant on 17 May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

style

left-column

性能和储存信息

形式

Liquid

纯度

Tissue culture supernatant

纯化说明

Purified from TCS.

存储溶液

Preservative: 0.1% Sodium azide Constituents: 0.82% Sodium phosphate, 0.2% Gelatin

运输条件

Blue Ice

储存信息

Avoid freeze / thaw cycle

style

left-column

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The Ras protein also known by its alternate names such as KRAS NRAS and HRAS functions as a small GTPase which is a type of enzyme that binds to guanosine triphosphate (GTP) and can switch to an inactive form when it hydrolyzes GTP to GDP. Ras proteins are encoded by genes located in different chromosomes and typically have a molecular weight of about 21 kDa. These proteins are expressed in various tissues throughout the body where they play key roles in cellular signaling.

Biological function summary

Ras proteins are pivotal in regulating cell proliferation differentiation and survival. They are often part of larger protein complexes that facilitate signal transduction across cell membranes. These proteins function as binary molecular switches toggling between active (GTP-bound) and inactive (GDP-bound) states. Mutations in Ras proteins such as NRAS Q61R can lead the protein to assume permanently active conformations disrupting normal cellular signaling processes and contributing to oncogenesis.

Pathways

Ras proteins play significant roles in the MAPK/ERK and PI3K/AKT signaling pathways. Their activation leads to a cascade of phosphorylation events that ultimately regulate gene expression. Within these pathways Ras proteins interact with various molecules including RAF kinases and the PI3K protein further illustrating their complex roles in signal propagation. These pathways control many cellular processes including growth differentiation and survival illustrating how Ras proteins integrate multiple signals to modulate cellular outcomes.

Ras proteins are frequently implicated in various cancers notably pancreatic and colorectal cancers. Mutated forms of Ras such as KRAS G12V drive oncogenesis by promoting uncontrolled cell growth and division. In cancer pathology Ras often interacts with tumor suppressor proteins influencing the disease progression. Targeting Ras-mediated signaling pathways using approaches like pan-Ras inhibitors or chemiluminescence ELISAs represents an ongoing area of therapeutic research aimed at combating Ras-driven malignancies.

style

left-column

style

left-column

产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

靶点信息

Involved in the activation of Ras protein signal transduction (PubMed : 22821884). Ras proteins bind GDP/GTP and possess intrinsic GTPase activity (PubMed : 12740440, PubMed : 14500341, PubMed : 9020151).

See full target information HRAS

style

left-column

文献 (12)

Recent publications for all applications. Explore the full list and refine your search

Aging 12:16021-16034 PubMed32756011

2020

Long non-coding RNA DLX6-AS1 facilitates bladder cancer progression through modulating miR-195-5p/VEGFA signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Hengbing Wang,Xiaobing Niu,Hesong Jiang,Fei Mao,Bing Zhong,Xi Jiang,Guangbo Fu

BMC cancer 19:1160 PubMed31783811

2019

Syndecan-1 suppresses cell growth and migration via blocking JAK1/STAT3 and Ras/Raf/MEK/ERK pathways in human colorectal carcinoma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Shaojun Wang,Xiaofei Zhang,Guimei Wang,Bin Cao,Hong Yang,Lipeng Jin,Mingjuan Cui,Yongjun Mao

Phytomedicine : international journal of phytother 61:152857 PubMed31035040

2019

Chinese yam extract and adenosine attenuated LPS-induced cardiac dysfunction by inhibiting RAS and apoptosis via the ER-mediated activation of SHC/Ras/Raf1 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Mengnan Zeng,Li Zhang,Beibei Zhang,Benke Li,Yuxuan Kan,Hang Yang,Weisheng Feng,Xiaoke Zheng

Cell death & disease 9:1170 PubMed30518913

2018

UBIAD1 suppresses the proliferation of bladder carcinoma cells by regulating H-Ras intracellular trafficking via interaction with the C-terminal domain of H-Ras.

Applications

Unspecified application

Species

Unspecified reactive species

Zhiliang Xu,Fengsen Duan,Huiai Lu,Maytham Abdulkadhim Dragh,Yanzhi Xia,Huageng Liang,Ling Hong

Cell proliferation 52:e12557 PubMed30485569

2018

Matrix stiffness regulates arteriovenous differentiation of endothelial progenitor cells during vasculogenesis in nude mice.

Applications

Unspecified application

Species

Unspecified reactive species

Changyue Xue,Qian Huang,Tao Zhang,Dan Zhao,Quanquan Ma,Taoran Tian,Xiaoxiao Cai

Molecular medicine reports 18:3683-3690 PubMed30106104

2018

In vitro and in vivo study of the expression of the Syk/Ras/c‑Fos pathway in chronic glomerulonephritis.

Applications

Unspecified application

Species

Unspecified reactive species

Jiarong Gao,Liangbing Wei,Junmei Song,Hui Jiang,Yachen Gao,Xi Wu,Shuangzhi Xu

Oncotarget 9:4440-4450 PubMed29435114

2018

RAS GTPases are modified by SUMOylation.

Applications

Unspecified application

Species

Unspecified reactive species

Byeong Hyeok Choi,Changyan Chen,Mark Philips,Wei Dai

Oncotarget 7:47954-47965 PubMed27323830

2016

Vitamin C uncouples the Warburg metabolic switch in KRAS mutant colon cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Oscar Aguilera,María Muñoz-Sagastibelza,Blanca Torrejón,Aurea Borrero-Palacios,Laura Del Puerto-Nevado,Javier Martínez-Useros,María Rodriguez-Remirez,Sandra Zazo,Estela García,Mario Fraga,Federico Rojo,Jesús García-Foncillas

Nature medicine 20:769-77 PubMed24859528

2014

Oncogenic transformation of diverse gastrointestinal tissues in primary organoid culture.

Applications

IHC-P

Species

Mouse

Xingnan Li,Lincoln Nadauld,Akifumi Ootani,David C Corney,Reetesh K Pai,Olivier Gevaert,Michael A Cantrell,Paul G Rack,James T Neal,Carol W-M Chan,Trevor Yeung,Xue Gong,Jenny Yuan,Julie Wilhelmy,Sylvie Robine,Laura D Attardi,Sylvia K Plevritis,Kenneth E Hung,Chang-Zheng Chen,Hanlee P Ji,Calvin J Kuo

Oncology reports 27:1893-903 PubMed22426690

2012

Angiotensin II receptor type 1 blockers suppress the cell proliferation effects of angiotensin II in breast cancer cells by inhibiting AT1R signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Ning Du,Jiang Feng,Li-Juan Hu,Xin Sun,Hai-Bing Sun,Yang Zhao,Yi-Ping Yang,Hong Ren

View all publications

style

left-column

style

left-column

style

right-column