重组Anti-Ras抗体[EPR3255] (ab108602)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ras antibody [EPR3255] (ab108602)](https://www.abcam.cn/ps/products/108/ab108602/Images/ab108602-515537-anti-ras-antibody-epr3255-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-rat-cardiac-muscle.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ras antibody [EPR3255] (ab108602)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3255] to Ras
- Suitable for: IHC-P, Flow Cyt (Intra), WB, IP, ICC/IF
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-Ras抗体[EPR3255]
参阅全部 Ras 一抗 -
描述
兔单克隆抗体[EPR3255] to Ras -
宿主
Rabbit -
特异性
ab108602 also detects H-Ras and N-Ras. -
经测试应用
适用于: IHC-P, Flow Cyt (Intra), WB, IP, ICC/IFmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: 293T and SH-SY5Y cell lysates; Rat brain lysates; Mouse heart lysates IP: SH-SY5Y cell lysates Flow Cyt (intra): HEK-293 cells ICC/IF: HEK-293T cells IHC-P: Human breast carcinoma, mouse cerebrum, rat cerebrum and rat cardiac muscle tissues.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR3255 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab108602于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
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| Flow Cyt (Intra) |
1/30.
|
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| WB | (6) |
1/1000 - 1/10000. Predicted molecular weight: 22 kDa.
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| IP | (2) |
1/10 - 1/100.
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| ICC/IF |
1/50 - 1/100.
|
| 说明 |
|---|
|
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
|
Flow Cyt (Intra)
1/30. |
|
WB
1/1000 - 1/10000. Predicted molecular weight: 22 kDa. |
|
IP
1/10 - 1/100. |
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ICC/IF
1/50 - 1/100. |
靶标
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功能
Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. -
疾病相关
Defects in HRAS are the cause of faciocutaneoskeletal syndrome (FCSS) [MIM:218040]. A rare condition characterized by prenatally increased growth, postnatal growth deficiency, mental retardation, distinctive facial appearance, cardiovascular abnormalities (typically pulmonic stenosis, hypertrophic cardiomyopathy and/or atrial tachycardia), tumor predisposition, skin and musculoskeletal abnormalities.
Defects in HRAS are the cause of congenital myopathy with excess of muscle spindles (CMEMS) [MIM:218040]. CMEMS is a variant of Costello syndrome.
Defects in HRAS may be a cause of susceptibility to Hurthle cell thyroid carcinoma (HCTC) [MIM:607464]. Hurthle cell thyroid carcinoma accounts for approximately 3% of all thyroid cancers. Although they are classified as variants of follicular neoplasms, they are more often multifocal and somewhat more aggressive and are less likely to take up iodine than are other follicular neoplasms.
Note=Mutations which change positions 12, 13 or 61 activate the potential of HRAS to transform cultured cells and are implicated in a variety of human tumors.
Defects in HRAS are a cause of susceptibility to bladder cancer (BLC) [MIM:109800]. A malignancy originating in tissues of the urinary bladder. It often presents with multiple tumors appearing at different times and at different sites in the bladder. Most bladder cancers are transitional cell carcinomas. They begin in cells that normally make up the inner lining of the bladder. Other types of bladder cancer include squamous cell carcinoma (cancer that begins in thin, flat cells) and adenocarcinoma (cancer that begins in cells that make and release mucus and other fluids). Bladder cancer is a complex disorder with both genetic and environmental influences.
Note=Defects in HRAS are the cause of oral squamous cell carcinoma (OSCC). -
序列相似性
Belongs to the small GTPase superfamily. Ras family. -
翻译后修饰
Palmitoylated by the ZDHHC9-GOLGA7 complex. A continuous cycle of de- and re-palmitoylation regulates rapid exchange between plasma membrane and Golgi.
S-nitrosylated; critical for redox regulation. Important for stimulating guanine nucleotide exchange. No structural perturbation on nitrosylation. -
细胞定位
Cell membrane. Golgi apparatus membrane. The active GTP-bound form is localized most strongly to membranes than the inactive GDP-bound form (By similarity). Shuttles between the plasma membrane and the Golgi apparatus. - Information by UniProt
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数据库链接
- Entrez Gene: 3265 Human
- Entrez Gene: 3845 Human
- Entrez Gene: 4893 Human
- Entrez Gene: 15461 Mouse
- Entrez Gene: 16653 Mouse
- Entrez Gene: 18176 Mouse
- Entrez Gene: 24525 Rat
- Entrez Gene: 24605 Rat
see all -
别名
- C BAS/HAS antibody
- C HA RAS1 antibody
- C-BAS/HAS antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ras antibody [EPR3255] (ab108602)
Immunohistochemical analysis of paraffin-embedded rat cardiac muscle tissue labelling Ras with ab209974 at 1/10000 (0.104 μg/ml) dilution followed by a LeicaDS9800 (Bond™ Polymer Refine Detection) at ready to use dilution. Positive staining on rat cardiac muscle. The section was incubated with ab209974 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection) at ready to use dilution.
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
This data was developed using the same antibody clone in a different buffer formulation (ab209974).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ras antibody [EPR3255] (ab108602)](https://www.abcam.cn/ps/products/108/ab108602/Images/ab108602-515536-anti-ras-antibody-epr3255-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-rat-cerebrum.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ras antibody [EPR3255] (ab108602)Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labelling Ras with ab209974 at 1/10000 (0.104 μg/ml) dilution followed by a LeicaDS9800 (Bond™ Polymer Refine Detection) at ready to use dilution. Positive staining on rat cerebrum. The section was incubated with ab209974 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
Secondary antibody only control: Secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection) at Ready to use dilution.
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
This data was developed using the same antibody clone in a different buffer formulation (ab209974).
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ras antibody [EPR3255] (ab108602)](https://www.abcam.cn/ps/products/108/ab108602/Images/ab108602-515535-anti-ras-antibody-epr3255-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-mouse-cerebrum.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ras antibody [EPR3255] (ab108602)Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labelling Ras with ab209974 at 1/10000 (0.104 μg/ml) dilution followed by a LeicaDS9800 (Bond™ Polymer Refine Detection) at ready to use dilution. Positive staining on mouse cerebrum. The section was incubated with ab209974 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
Secondary antibody only control: Secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection) at ready to use dilution.
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
This data was developed using the same antibody clone in a different buffer formulation (ab209974).
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ras antibody [EPR3255] (ab108602)](https://www.abcam.cn/ps/products/108/ab108602/Images/ab108602-515534-anti-ras-antibody-epr3255-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-human-breast-carcinoma.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ras antibody [EPR3255] (ab108602)Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue labelling Ras with ab209974 at 1/10000 (0.104 μg/ml) dilution followed by a LeicaDS9800 (Bond™ Polymer Refine Detection) at ready to use dilution. Positive staining on human breast carcinoma. The section was incubated with ab209974 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
Secondary antibody only control: Secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection) at ready to use dilution.
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
This data was developed using the same antibody clone in a different buffer formulation (ab209974).
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![Western blot - Anti-Ras antibody [EPR3255] (ab108602)](https://www.abcam.cn/ps/products/108/ab108602/Images/ab108602-351353-anti-ras-antibody-epr3255-westernblot-human-mouse-rat.jpg)
Western blot - Anti-Ras antibody [EPR3255] (ab108602)All lanes : Anti-Ras antibody [EPR3255] (ab108602) at 1/10000 dilution (Purified)
Lane 1 : HEK-293 (Human embryonic kidney epithelial cell) whole cell lysates
Lane 2 : Rat brain lysates
Lane 3 : Mouse heart lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 22 kDa
Observed band size: 21 kDa why is the actual band size different from the predicted? -
![Immunoprecipitation - Anti-Ras antibody [EPR3255] (ab108602)](https://www.abcam.cn/ps/products/108/ab108602/Images/ab108602-4-anti-ras-antibody-epr3255-immunoprecipitation-shsy5y-human.jpg)
Immunoprecipitation - Anti-Ras antibody [EPR3255] (ab108602)ab108602 (purified) at 1/20 dilution (2 µg) immunoprecipitating Ras in SH-SY5Y whole cell lysate.
Lane 1 (input): SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysate 10 µg
Lane 2 (+): ab108602 & SH-SY5Y whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab108602 in SH-SY5Y whole cell lysate
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/1000 dilution.
Blocking and diluting buffer: 5% NFDM/TBST. -
![Flow Cytometry (Intracellular) - Anti-Ras antibody [EPR3255] (ab108602)](https://www.abcam.cn/ps/products/108/ab108602/Images/ab108602-3-anti-ras-antibody-epr3255-flowcytometry-hek293-human.jpg)
Flow Cytometry (Intracellular) - Anti-Ras antibody [EPR3255] (ab108602)Intracellular Flow Cytometry analysis of HEK-293 (Human embryonic kidney epithelial cell) cells labeling Ras with purified ab108602 at 1/30 dilution (10 µg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
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![Immunocytochemistry/ Immunofluorescence - Anti-Ras antibody [EPR3255] (ab108602)](https://www.abcam.cn/ps/products/108/ab108602/Images/ab108602-2-anti-ras-antibody-epr3255-immunocytochemistry-293t-human.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Ras antibody [EPR3255] (ab108602)Immunocytochemistry/ Immunofluorescence analysis of 293T (Human embryonic kidney epithelial cell) cells labeling Ras with purified ab108602 at 1:100 dilution (10 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control. -
![Western blot - Anti-Ras antibody [EPR3255] (ab108602)](https://www.abcam.cn/ps/products/108/ab108602/Images/ab108602-121318-anti-ras-antibody-epr3255-western-blot.jpg)
Western blot - Anti-Ras antibody [EPR3255] (ab108602)All lanes : Anti-Ras antibody [EPR3255] (ab108602) at 1/1000 dilution (unpurified)
Lane 1 : HEK-293 whole cell lysate
Lane 2 : SH-SY5Y cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 22 kDa -
![Anti-Ras antibody [EPR3255] (ab108602)](https://www.abcam.cn/ps/products/108/ab108602/Images/ab108602-6-benefits-of-recombinant-antibodies.png)
Anti-Ras antibody [EPR3255] (ab108602)
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (27)
ab108602 被引用在 27 文献中.
- Chang Y et al. Parvimonas micra activates the Ras/ERK/c-Fos pathway by upregulating miR-218-5p to promote colorectal cancer progression. J Exp Clin Cancer Res 42:13 (2023). PubMed: 36627634
- Lu JH et al. Effects of Therapeutic Platelet-Rich Plasma on Overactive Bladder via Modulating Hyaluronan Synthesis in Ovariectomized Rat. Int J Mol Sci 24:N/A (2023). PubMed: 37175945
- Li L et al. CD59-Regulated Ras Compartmentalization Orchestrates Antitumor T-cell Immunity. Cancer Immunol Res 10:1475-1489 (2022). PubMed: 36206575
- Liu S et al. Development of a biotin-streptavidin-enhanced enzyme-linked immunosorbent assay (BA-ELISA) for high-throughput screening of KRASG12C inhibitors. SLAS Discov 27:107-113 (2022). PubMed: 35058184
- Hong SH et al. A Sos proteomimetic as a pan-Ras inhibitor. Proc Natl Acad Sci U S A 118:N/A (2021). PubMed: 33926964