Anti-RAP80抗体(ab70822)
Key features and details
- Rabbit polyclonal to RAP80
- Suitable for: IP, WB
- Reacts with: Human
- Isotype: IgG
概述
-
产品名称
Anti-RAP80抗体
参阅全部 RAP80 一抗 -
描述
兔多克隆抗体to RAP80 -
宿主
Rabbit -
经测试应用
适用于: IP, WBmore details -
种属反应性
与反应: Human
预测可用于: Chimpanzee, Gorilla
-
免疫原
Synthetic peptide corresponding to a region between residues 600 and 650 of human RAP80 (NP_057374.3).
-
阳性对照
- HeLa and 293T whole cell lysates.
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab70822于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IP |
Use at 2-5 µg/mg of lysate.
|
|
| WB |
1/1000 - 1/10000. Detects a band of approximately 100 kDa (predicted molecular weight: 80 kDa).
|
| 说明 |
|---|
|
IP
Use at 2-5 µg/mg of lysate. |
|
WB
1/1000 - 1/10000. Detects a band of approximately 100 kDa (predicted molecular weight: 80 kDa). |
靶标
-
功能
Ubiquitin-binding protein that specifically recognizes and binds 'Lys-63'-linked ubiquitin. Plays a central role in the BRCA1-A complex by specifically binding 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks (DSBs). The BRCA1-A complex also possesses deubiquitinase activity that specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX. Also weakly binds monoubiquitin but with much less affinity than 'Lys-63'-linked ubiquitin. May interact with monoubiquitinated histones H2A and H2B; the relevance of such results is however unclear in vivo. Does not bind Lys-48'-linked ubiquitin. May indirectly Act as a transcriptional repressor by inhibiting the interaction of NR6A1 with the corepressor NCOR1. -
组织特异性
Expressed in testis, ovary, thymus and heart. Expressed in germ cells of the testis. -
序列相似性
Belongs to the RAP80 family.
Contains 2 UIM (ubiquitin-interacting motif) repeats. -
结构域
The UIM-linker region between the 2 UIM repeats determines the selectivity for 'Lys-63'-linked ubiquitin. The length of the linker is important. The linker reduces the flexibility between the UIM repeats and promotes high-affinity and linkage-selective interactions.
The Abraxas-interacting region (AIR) mediates the interaction with FAM175A/Abraxas. -
翻译后修饰
Sumoylated.
Phosphorylated upon DNA damage by ATM or ATR. -
细胞定位
Nucleus. Localizes at sites of DNA damage at double-strand breaks. - Information by UniProt
-
数据库链接
- Entrez Gene: 462299 Chimpanzee
- Entrez Gene: 51720 Human
- SwissProt: Q96RL1 Human
- Unigene: 232721 Human
-
别名
- BRCA1-A complex subunit RAP80 antibody
- Nuclear zinc finger protein RAP80 antibody
- OTTHUMP00000161441 antibody
see all
图片
-
Western blot - Anti-RAP80 antibody (ab70822)All lanes : Anti-RAP80 antibody (ab70822) at 1 µg/ml
Lane 1 : HeLa whole cell lysate at 15 µg
Lane 2 : HeLa whole cell lysate at 5 µg
Lane 3 : 293T whole cell lysate at 50 µg
Predicted band size: 80 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?
Additional bands at: 300 kDa, 65 kDa, 80 kDa. We are unsure as to the identity of these extra bands. -
Immunoprecipitation - Anti-RAP80 antibody (ab70822)Detection of Human RAP80 by Immunoprecipitation in Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded), using ab70822 at 3 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection.
数据表及文件
-
SDS download
-
Datasheet download
文献 (1)
ab70822 被引用在 1 文献中.
- Goldstein M & Kastan MB Repair versus Checkpoint Functions of BRCA1 Are Differentially Regulated by Site of Chromatin Binding. Cancer Res 75:2699-707 (2015). PubMed: 25939603