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AB221041

Anti-RAP55B抗体

Anti-RAP55B antibody

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(2 Publications)

Rabbit Polyclonal RAP55B antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human LSM14B aa 50-200.

查看别名

C20orf40, FAM61B, RAP55B, LSM14B, Protein LSM14 homolog B, RNA-associated protein 55B, hRAP55B

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAP55B antibody (AB221041)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAP55B antibody (AB221041)

Immunohistochemical analysis of formalin/PFA-fixed paraffin-embedded human testis tissue sections labeling RAP55B with ab221041 at a 1/50 dilution.

Immunocytochemistry/ Immunofluorescence - Anti-RAP55B antibody (AB221041)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-RAP55B antibody (AB221041)

Immunofluorescent analysis of PFA fixed, triton X-100 SiHa cells labeling RAP55B with ab221041 at 4 μg/mL (green).

Western blot - Anti-RAP55B antibody (AB221041)
  • WB

Supplier Data

Western blot - Anti-RAP55B antibody (AB221041)

All lanes:

Western blot - Anti-RAP55B antibody (ab221041) at 1/100 dilution

Lane 1:

Vector only transfected HEK293T lysate

Lane 2:

RAP55B over-expression lysate (co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells

Predicted band size: 42 kDa

false

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

No

反应种属

Human

应用

WB, ICC/IF, IHC-P

applications

免疫原

Recombinant Fragment Protein within Human LSM14B aa 50-200. The exact immunogen used to generate this antibody is proprietary information.

Q9BX40

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Immunogen
存储溶液
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle

产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

靶点信息

mRNA-binding protein essential for female fertility, oocyte meiotic maturation and the assembly of MARDO (mitochondria-associated ribonucleoprotein domain), a membraneless compartment that stores maternal mRNAs in oocytes. Ensures the proper accumulation and clearance of mRNAs essential for oocyte meiotic maturation and the normal progression from Meiosis I to Meiosis II in oocytes. Promotes the translation of some oogenesis-related mRNAs. Regulates the expression and/or localization of some key P-body proteins in oocytes. Essential for the assembly of the primordial follicle in the ovary.
See full target information LSM14B

文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Journal of cell science 135: PubMed34897463

2022

Comparative analysis of vertebrates reveals that mouse primordial oocytes do not contain a Balbiani body.

Applications

Unspecified application

Species

Unspecified reactive species

Laasya Dhandapani,Marion C Salzer,Juan M Duran,Gabriele Zaffagnini,Cristian De Guirior,Maria Angeles Martínez-Zamora,Elvan Böke

Molecular medicine reports 22:5339-5347 PubMed33174007

2020

Endothelial cell‑derived CCL15 mediates the transmigration of fibrocytes through the CCL15‑CCR1 axis in vitro.

Applications

Unspecified application

Species

Unspecified reactive species

Nan Pang,Zhixiao Lin,Xiaolin Wang,Lirong Xu,Xiaoli Xu,Rong Huang,Xingxing Li,Xueyong Li,Jinqing Li
View all publications

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