Anti-RAP1/TERF2IP抗体(ab272863)
Key features and details
- Rabbit polyclonal to RAP1/TERF2IP
- Suitable for: WB, ICC/IF, IHC-P, IP
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-RAP1/TERF2IP抗体
参阅全部 RAP1/TERF2IP 一抗 -
描述
兔多克隆抗体to RAP1/TERF2IP -
宿主
Rabbit -
经测试应用
适用于: WB, ICC/IF, IHC-P, IPmore details -
种属反应性
与反应: Mouse, Human -
免疫原
Recombinant fragment within Human RAP1/TERF2IP. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. Center region.
Database link: Q9NYB0 -
阳性对照
- WB: HepG2 and HCT116 whole cell lysate. Mouse brain whole cell lysate. IHC-P: SCM1 xenograft tissue. ICC/IF: A-375 cells. IP: HepG2 whole cell lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab272863于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/500 - 1/3000.
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| ICC/IF |
1/100 - 1/1000.
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| IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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| IP |
1/100 - 1/500.
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| 说明 |
|---|
|
WB
1/500 - 1/3000. |
|
ICC/IF
1/100 - 1/1000. |
|
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
|
IP
1/100 - 1/500. |
靶标
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功能
Acts both as a regulator of telomere function and as a transcription regulator. Involved in the regulation of telomere length and protection as a component of the shelterin complex (telosome). In contrast to other components of the shelterin complex, it is dispensible for telomere capping and does not participate in the protection of telomeres against non-homologous end-joining (NHEJ)-mediated repair. Instead, it is required to negatively regulate telomere recombination and is essential for repressing homology-directed repair (HDR), which can affect telomere length. Does not bind DNA directly: recruited to telomeric double-stranded 5'-TTAGGG-3' repeats via its interaction with TERF2. Independently of its function in telomeres, also acts as a transcription regulator: recruited to extratelomeric 5'-TTAGGG-3' sites via its association with TERF2 or other factors, and regulates gene expression. When cytoplasmic, associates with the I-kappa-B-kinase (IKK) complex and acts as a regulator of the NF-kappa-B signaling by promoting IKK-mediated phosphorylation of RELA/p65, leading to activate expression of NF-kappa-B target genes. -
组织特异性
Ubiquitous. Highly expressed. -
序列相似性
Belongs to the RAP1 family.
Contains 1 BRCT domain.
Contains 1 Myb-like domain. -
翻译后修饰
Phosphorylated upon DNA damage, probably by ATM or ATR. -
细胞定位
Nucleus. Cytoplasm. Chromosome. Chromosome > telomere. Associates with chromosomes, both at telomeres and in extratelomeric sites. Also exists as a cytoplasmic form, where it associates with the IKK complex. - Information by UniProt
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数据库链接
- Entrez Gene: 54386 Human
- Entrez Gene: 57321 Mouse
- Omim: 605061 Human
- SwissProt: Q9NYB0 Human
- SwissProt: Q91VL8 Mouse
- Unigene: 301419 Human
- Unigene: 213064 Mouse
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别名
- Dopamine receptor interacting protein 5 antibody
- Dopamine receptor-interacting protein 5 antibody
- DRIP 5 antibody
see all
图片
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Western blot - Anti-RAP1/TERF2IP antibody (ab272863)All lanes : Anti-RAP1/TERF2IP antibody (ab272863) at 1/1000 dilution
Lane 1 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 2 : HCT 116 (human colorectal carcinoma cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.10% SDS PAGE
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAP1/TERF2IP antibody (ab272863)Paraffin-embedded SCM1 xenograft tissue stained for RAP1/TERF2IP using ab272863 at 1/500 dilution in immunohistochemical analysis. Antigen retrieval using EDTA based, pH 8.0 buffer, 15 minutes.
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Immunocytochemistry/ Immunofluorescence - Anti-RAP1/TERF2IP antibody (ab272863)4% PFA-fixed A-375 (human malignant melanoma cell line) cells stained for RAP1/TERF2IP using ab272863 at 1/500 dilution in ICC/IF.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.
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Immunoprecipitation - Anti-RAP1/TERF2IP antibody (ab272863)IP Sample: HepG2 whole cell lysate/extract.
10% SDS-PAGE
All lanes : Anti-RAP1/TERF2IP antibody (ab272863) at 1/1000 dilution
Lane 1 : Control with 3 µg of pre-immune rabbit IgG
Lane 2 : Immunoprecipitation of RAP1/TERF2IP by 3 µg of ab272863
Secondary
All lanes : anti-rabbit IgG (HRP) -
Western blot - Anti-RAP1/TERF2IP antibody (ab272863)Anti-RAP1/TERF2IP antibody (ab272863) at 1/1000 dilution + Mouse brain whole cell lysate at 50 µg10% SDS PAGE
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab272863 被引用在 1 文献中.
- Yang JF et al. SOX9 promotes the invasion and migration of lung adenocarcinoma cells by activating the RAP1 signaling pathway. BMC Pulm Med 23:421 (2023). PubMed: 37919693